##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921166_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2293618
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.191753814279448	31.0	26.0	31.0	2.0	34.0
2	25.811736740817345	31.0	26.0	31.0	2.0	34.0
3	25.589324813460653	31.0	25.0	31.0	2.0	34.0
4	29.37531009958938	35.0	28.0	37.0	2.0	37.0
5	28.983900544903292	35.0	28.0	37.0	2.0	37.0
6	29.442592009654614	35.0	30.0	37.0	2.0	37.0
7	29.259140362518956	35.0	29.0	37.0	2.0	37.0
8	28.964443512389597	35.0	28.0	37.0	2.0	37.0
9	30.55326737059092	37.0	30.0	39.0	2.0	39.0
10	29.913753292832546	37.0	28.0	39.0	2.0	39.0
11	30.36609758032942	37.0	30.0	39.0	2.0	39.0
12	30.079850262772617	37.0	29.0	39.0	2.0	39.0
13	30.064201187817677	37.0	29.0	39.0	2.0	39.0
14	31.096467676831974	38.0	30.0	40.0	2.0	41.0
15	30.76156491621534	37.0	28.0	40.0	2.0	41.0
16	30.668871625527878	37.0	28.0	40.0	2.0	41.0
17	30.966686257258182	38.0	30.0	40.0	2.0	41.0
18	31.01383185866173	38.0	30.0	40.0	2.0	41.0
19	30.705872555935645	38.0	29.0	40.0	2.0	41.0
20	30.59588606297997	38.0	28.0	40.0	2.0	41.0
21	30.528084013990124	38.0	28.0	40.0	2.0	41.0
22	29.787632029396352	37.0	27.0	40.0	2.0	41.0
23	29.86339181153967	37.0	27.0	40.0	2.0	41.0
24	29.51487649643489	37.0	26.0	40.0	2.0	41.0
25	29.035141858844845	37.0	24.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	256579.0
3	11.0
4	2012.0
5	10723.0
6	12135.0
7	10271.0
8	8901.0
9	8060.0
10	7703.0
11	7299.0
12	7161.0
13	6888.0
14	6846.0
15	6878.0
16	7243.0
17	8044.0
18	9239.0
19	11234.0
20	14217.0
21	17637.0
22	21317.0
23	24528.0
24	27788.0
25	31164.0
26	34627.0
27	38615.0
28	42896.0
29	49665.0
30	58396.0
31	69594.0
32	86665.0
33	109520.0
34	139363.0
35	179734.0
36	235264.0
37	305166.0
38	420235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93292920940258	17.811284678835744	12.149319125755987	26.106466986005692
2	15.922513820770787	20.33996936812042	38.293382912017755	25.44413389909103
3	19.419506979486258	23.303879314103916	29.4558215874821	27.820792118927724
4	13.804417828811422	15.853612274236948	34.17466465566897	36.16730524128266
5	14.176195426195425	36.24169794471808	33.655197854023356	15.926908775063136
6	33.83512113494992	33.936664604405564	17.217438619392492	15.010775641252025
7	29.15878576056521	31.250812071237288	20.285910117121382	19.30449205107612
8	26.64260516479353	33.974285468878826	20.442873480478223	18.94023588584942
9	26.88834793346161	15.906923691711217	19.109403075597577	38.0953252992296
10	16.146756670262278	27.90012635500262	32.554899897015005	23.398217077720098
11	35.40565394783487	22.56237651910659	21.8535509518521	20.178418581206444
12	23.27458779433451	24.6959746122995	29.131932847125263	22.897504746240724
13	28.619647831836932	21.29630638970294	24.897913266500773	25.186132511959354
14	22.850963257546024	20.845303192834656	25.612272485457382	30.691461064161935
15	24.703002027513136	27.90477228629358	22.92428437506813	24.46794131112516
16	24.327730835891224	26.99242794951036	24.70816509704279	23.971676117555628
17	23.31388179998578	27.443672479736836	25.50421837591982	23.738227344357565
18	23.53395741444469	26.721463465263717	26.231467049965158	23.513112070326432
19	24.343486627714043	26.362662343183995	26.47237740236079	22.82147362674117
20	24.817037939853527	25.61128711187471	26.14638273007345	23.425292218198308
21	25.12848445256356	25.30902773083701	25.712764366164446	23.849723450434983
22	24.284879614207355	24.960627174661866	26.83216624663173	23.922326964499057
23	24.620243035735857	25.265442868461825	25.94060027655159	24.17371381925073
24	24.29128754498334	25.667920582519276	25.924924640776435	24.115867231720948
25	23.957047864243076	25.130556566772988	26.621361205260204	24.29103436372374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1345.0
1	1345.0
2	1371.0
3	1397.0
4	1397.0
5	1397.0
6	1873.5
7	2350.0
8	2350.0
9	2350.0
10	2965.0
11	3580.0
12	3580.0
13	3580.0
14	5139.0
15	6698.0
16	6698.0
17	6698.0
18	11410.0
19	16122.0
20	16122.0
21	16122.0
22	25035.0
23	33948.0
24	33948.0
25	33948.0
26	50548.5
27	67149.0
28	67149.0
29	67149.0
30	89037.0
31	110925.0
32	110925.0
33	110925.0
34	135353.0
35	159781.0
36	159781.0
37	159781.0
38	188016.0
39	216251.0
40	216251.0
41	216251.0
42	242681.0
43	269111.0
44	269111.0
45	269111.0
46	288601.0
47	308091.0
48	308091.0
49	308091.0
50	309470.0
51	310849.0
52	310849.0
53	310849.0
54	291552.0
55	272255.0
56	272255.0
57	272255.0
58	241594.5
59	210934.0
60	210934.0
61	210934.0
62	177983.5
63	145033.0
64	145033.0
65	145033.0
66	114753.0
67	84473.0
68	84473.0
69	84473.0
70	63662.5
71	42852.0
72	42852.0
73	42852.0
74	30860.5
75	18869.0
76	18869.0
77	18869.0
78	13202.5
79	7536.0
80	7536.0
81	7536.0
82	5196.5
83	2857.0
84	2857.0
85	2857.0
86	1874.0
87	891.0
88	891.0
89	891.0
90	574.0
91	257.0
92	257.0
93	257.0
94	156.5
95	56.0
96	56.0
97	56.0
98	32.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033004624135318086
2	0.05384506051138419
3	0.014213352005434209
4	0.009984225795228324
5	0.009155840248899337
6	8.283855463289878E-4
7	0.004359923928047303
8	0.0022235612033041246
9	0.00632188969566859
10	0.0034879391424378426
11	0.01076901210227684
12	0.009766229598825959
13	0.009809828838106433
14	0.015608527662409347
15	0.00732467219911947
16	0.0310426583676968
17	0.039718906984510936
18	0.02354358921145544
19	0.01765769190859158
20	0.016698508644421173
21	0.023499989972174968
22	0.006888679806314739
23	0.01634971473017739
24	0.011728195366447246
25	0.47483931500363186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2293618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.72321840374747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85310017666163	35.496375112975784
2	10.685910081547654	10.413038614921064
3	5.310151536278386	7.761830191772607
4	3.1075935566667283	6.056478382862056
5	2.0151282678609155	4.909176735327636
6	1.3544912080686196	3.959710257400986
7	0.9821587535114988	3.3497754818145196
8	0.6888176711760754	2.6849131066458103
9	0.5456867223820737	2.39288520191822
>10	2.3712022280442535	18.768367253976788
>50	0.06802319228026388	2.222036679378532
>100	0.016168350376059203	1.2134428207905101
>500	0.001014753329660254	0.36110674044669794
>1k	5.535018161783128E-4	0.4108634197688861
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2772	0.12085709128547126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7439695712189213E-4
2	0.0	4.3599239280473033E-5	0.0	0.0	1.7439695712189213E-4
3	0.0	4.3599239280473033E-5	0.0	0.0	1.7439695712189213E-4
4	4.3599239280473033E-5	4.3599239280473033E-5	0.0	0.0	1.7439695712189213E-4
5	4.3599239280473033E-5	4.3599239280473033E-5	0.0	0.0	1.7439695712189213E-4
6	4.3599239280473033E-5	4.3599239280473033E-5	0.0	0.0	3.923931535242573E-4
7	4.3599239280473033E-5	4.3599239280473033E-5	0.0	0.0	3.923931535242573E-4
8	4.3599239280473033E-5	4.3599239280473033E-5	0.0	0.0	5.667901106461494E-4
9	4.3599239280473033E-5	4.3599239280473033E-5	0.0	4.3599239280473033E-5	6.103893499266224E-4
10	4.3599239280473033E-5	4.3599239280473033E-5	0.0	4.3599239280473033E-5	6.539885892070955E-4
11	4.3599239280473033E-5	4.3599239280473033E-5	0.0	4.3599239280473033E-5	6.539885892070955E-4
12	4.3599239280473033E-5	4.3599239280473033E-5	0.0	4.3599239280473033E-5	7.411870677680415E-4
13	4.3599239280473033E-5	4.3599239280473033E-5	0.0	4.3599239280473033E-5	7.411870677680415E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTG	55	1.962625E-4	13.81666	14
CGTGTAA	60	4.103942E-4	12.662785	10
TAAACCG	55	0.0030807876	12.084832	5
CTAGCCG	75	2.0843436E-4	11.39427	4
GTACTAG	85	5.307528E-5	11.180116	1
GTATAGG	180	3.6379788E-12	11.086947	1
GTATTAC	105	3.4544282E-6	10.860684	1
GGTATCA	1305	0.0	10.122074	1
GTATCAA	2570	0.0	10.0577545	1
AGCGTGC	105	4.128278E-5	9.947597	8
GCTAGCC	220	1.8189894E-12	9.927259	3
AAGACCG	145	1.6297417E-7	9.822647	5
ACCGTGG	215	1.0913936E-11	9.716257	8
AGCCTCG	210	6.730261E-11	9.500611	2
TGCTAGC	240	1.8189894E-12	9.500611	2
CCTATAC	110	6.872925E-5	9.49564	3
ACCGTGT	150	2.6678026E-7	9.495432	8
TATACCG	80	0.004535599	9.495226	5
ACACCGG	90	0.0011190723	9.495225	6
TACACCG	90	0.0011190723	9.495225	5
>>END_MODULE