FastQCFastQC Report
Mon 15 Aug 2016
SRR2921166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293618
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47070.20522161929318658No Hit
GGTATCAACGCAGAGTACTTTTTTT30970.135026844051625No Hit
TATCAACGCAGAGTACTTTTTTTTT30870.13459085165882026No Hit
CTTATACACATCTCCGAGCCCACGA27970.12194707226748308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC5200.013.161693
CCGTGCG752.0749212E-411.399839
GGTATCA16450.011.3961231
CTTATAC5100.011.1953541
TACGATT600.00590740711.0756777
TGCACCG1158.835668E-710.7377995
TTATACA5550.010.6189412
CCCCTAG1659.0221874E-1010.3811461
TACGTAA750.002648718710.13318313
CCCCTAT1151.0064232E-59.9297921
CTATCGG900.00109686889.5160511
CCATTAG900.00109686889.5160511
TCTAATC1401.0503754E-69.5056643
CCGCTCG1106.839607E-59.4998589
GTATCAA30000.09.325731
ACCGACC1451.7132043E-69.1698758
CTAGATA950.0018142789.0053664
CGGTTTG950.00182439358.99986614
CGAGCAA950.00182439358.99986610
TGCTCGA1804.220783E-88.97208910
Produced by FastQC (version 0.11.5)