FastQCFastQC Report
Mon 15 Aug 2016
SRR2921165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947475
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37000.18998960192043543No Hit
TATCAACGCAGAGTACTTTTTTTTT21510.11045071181915044No Hit
GGTATCAACGCAGAGTACTTTTTTT20480.105161812090014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.013.2041761
TTGCGCG658.0435164E-411.68909918
CTATACG600.005891957311.0794384
ACCGATA803.7775838E-410.68566615
GTATTAC1201.5228052E-610.2922791
TTTGCGC750.002652424210.13133317
GTAATAC856.611198E-410.0560633
GTATAGG1901.0004442E-1010.0005951
GACGACA951.6467315E-49.9985413
ACTATCC951.6492943E-49.9969998
GTATCAA30300.09.8768251
GTCCTAA1451.6158265E-79.8281711
TAGGACA2450.09.6904714
GTTCTAA2002.6375346E-109.5005661
GTCTATC800.00451409639.5005661
ATAGGAC1951.6898412E-99.253873
GTGCTAT2304.1836756E-119.0874971
CTATACC1151.1149307E-49.0837634
AGGGTCG1054.507402E-49.0446715
CCGTCCA950.00182887678.9972999
Produced by FastQC (version 0.11.5)