##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921165_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1947475
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.595360145829858	31.0	27.0	31.0	2.0	34.0
2	26.28894696979422	31.0	26.0	31.0	2.0	34.0
3	25.944476309066868	31.0	26.0	31.0	2.0	34.0
4	29.685228821936096	35.0	30.0	37.0	2.0	37.0
5	29.479868034249478	35.0	29.0	37.0	2.0	37.0
6	29.84216485449107	35.0	30.0	37.0	2.0	37.0
7	29.730329272519544	35.0	30.0	37.0	2.0	37.0
8	29.396859523228795	35.0	30.0	37.0	2.0	37.0
9	30.910871256370427	37.0	30.0	39.0	2.0	39.0
10	30.23237166074019	37.0	28.0	39.0	2.0	39.0
11	30.74914183750754	37.0	30.0	39.0	2.0	39.0
12	30.44107677890602	37.0	30.0	39.0	2.0	39.0
13	30.459042092966534	37.0	30.0	39.0	2.0	39.0
14	31.549447361326845	38.0	30.0	40.0	2.0	41.0
15	31.20530995263097	38.0	30.0	40.0	2.0	41.0
16	31.154735234085162	38.0	30.0	40.0	2.0	41.0
17	31.479996919087537	38.0	30.0	40.0	2.0	41.0
18	31.531220169706927	38.0	31.0	40.0	2.0	41.0
19	31.219800510917985	38.0	30.0	40.0	2.0	41.0
20	31.072500545578247	38.0	30.0	40.0	2.0	41.0
21	31.017777378400236	38.0	30.0	40.0	2.0	41.0
22	30.281414652306193	37.0	27.0	40.0	2.0	41.0
23	30.39049127716659	37.0	28.0	40.0	2.0	41.0
24	30.0242354844093	37.0	27.0	40.0	2.0	41.0
25	29.532994775285946	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	199017.0
3	12.0
4	1455.0
5	8215.0
6	10363.0
7	8684.0
8	7206.0
9	6614.0
10	6147.0
11	5745.0
12	5749.0
13	5398.0
14	5461.0
15	5662.0
16	6069.0
17	6640.0
18	7520.0
19	9395.0
20	11648.0
21	14830.0
22	17732.0
23	20516.0
24	23411.0
25	26148.0
26	28925.0
27	32333.0
28	35815.0
29	42255.0
30	49807.0
31	58841.0
32	73227.0
33	92246.0
34	117438.0
35	151887.0
36	200558.0
37	262948.0
38	381558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.645016339449626	18.003751755424997	12.383823482578274	24.967408422547106
2	16.056766986245982	20.163500757547347	38.75621463687306	25.023517619333614
3	19.000565957695947	24.22535182333903	29.915332317827875	26.858749901137152
4	14.135009829317042	16.320671967855976	34.21536988839565	35.32894831443133
5	13.959732095079339	37.34805557352955	33.64361579205221	15.048596539338908
6	33.823049147195114	34.38532115744849	16.904618127444216	14.88701156791219
7	28.59111050175131	32.84668421828295	20.23293634226987	18.32926893769587
8	26.661672065218063	34.05236423687351	20.553538088029594	18.732425609878835
9	26.87171281351838	16.35841617308587	19.329760585497915	37.440110427897835
10	16.40513031971297	28.70951723911281	31.986240136222442	22.899112304951775
11	35.02854017578591	23.388667637263737	21.893126478042166	19.68966570890818
12	23.196521117531425	25.480410744767607	28.78546206732221	22.537606070378764
13	28.395953757225435	22.051232277916775	25.274689722321508	24.278124242536286
14	22.732910323009726	21.666893493327443	25.622048578567192	29.97814760509564
15	24.399989524176814	28.599356763915512	23.192445493624383	23.80820821828329
16	23.76714506511311	28.00821637903005	24.9285638338582	23.296074721998643
17	22.98977524442015	27.942339858053433	25.756325745589344	23.31155915193707
18	22.95879820875622	27.396692495789736	26.636610586293873	23.00789870916017
19	24.168622139911736	26.808754973424563	26.98614708343027	22.036475803233426
20	24.60453557292461	26.18603377978277	26.432394983208713	22.777035664083904
21	25.07240608010765	25.88616875662877	26.06757558403805	22.97384957922553
22	23.961884631994952	25.38910550794387	27.303860475076142	23.34514938498504
23	24.209262923704987	25.784189007564436	26.377931929872496	23.62861613885809
24	24.013007041201117	25.962830715885403	26.476785502448834	23.547376740464646
25	23.669267923235587	25.801142654313537	27.069526088405578	23.4600633340453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1942.0
1	1942.0
2	2008.0
3	2074.0
4	2074.0
5	2074.0
6	2708.0
7	3342.0
8	3342.0
9	3342.0
10	4156.0
11	4970.0
12	4970.0
13	4970.0
14	6845.0
15	8720.0
16	8720.0
17	8720.0
18	13603.0
19	18486.0
20	18486.0
21	18486.0
22	26810.0
23	35134.0
24	35134.0
25	35134.0
26	49550.5
27	63967.0
28	63967.0
29	63967.0
30	82718.0
31	101469.0
32	101469.0
33	101469.0
34	122170.0
35	142871.0
36	142871.0
37	142871.0
38	165112.0
39	187353.0
40	187353.0
41	187353.0
42	206602.5
43	225852.0
44	225852.0
45	225852.0
46	240959.0
47	256066.0
48	256066.0
49	256066.0
50	257517.0
51	258968.0
52	258968.0
53	258968.0
54	243822.0
55	228676.0
56	228676.0
57	228676.0
58	201557.0
59	174438.0
60	174438.0
61	174438.0
62	145647.0
63	116856.0
64	116856.0
65	116856.0
66	91098.5
67	65341.0
68	65341.0
69	65341.0
70	48613.0
71	31885.0
72	31885.0
73	31885.0
74	22042.0
75	12199.0
76	12199.0
77	12199.0
78	8324.5
79	4450.0
80	4450.0
81	4450.0
82	3009.5
83	1569.0
84	1569.0
85	1569.0
86	1104.0
87	639.0
88	639.0
89	639.0
90	411.0
91	183.0
92	183.0
93	183.0
94	100.5
95	18.0
96	18.0
97	18.0
98	12.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03353059731190387
2	0.054532150605270925
3	0.01709906417283919
4	0.012272301313238936
5	0.011142633409928241
6	9.756222801319658E-4
7	0.003902489120527863
8	0.0020025931013235086
9	0.007240144289400378
10	0.0036970949562895546
11	0.01129667903310697
12	0.009396783013902617
13	0.009602177178140926
14	0.016431533139064685
15	0.0068807045019833365
16	0.032452277949652754
17	0.0424652434562703
18	0.02300414639469056
19	0.01930705143840101
20	0.017150412713898767
21	0.02413381429800126
22	0.006367219091387566
23	0.01725310979601792
24	0.011399376115226127
25	0.47143095546797775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1947475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.98642140788713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6573183376794	35.59222014456568
2	11.24923761864325	11.021197890086297
3	5.472694626835269	8.042631756804962
4	3.0244151033788382	5.926210910659771
5	1.978396521734826	4.845728286280016
6	1.3046786906174783	3.834692408428691
7	0.9247937323921201	3.1711634843233236
8	0.6555272320454055	2.568954658665764
9	0.5058812709048284	2.2303181807011287
>10	2.1257264657753394	16.903770994423812
>50	0.07070596900386708	2.3678544750499326
>100	0.028344627864106325	2.3269307793351093
>500	0.0016284308037914801	0.5473885854496369
>1k	6.513723215165919E-4	0.6209374452258777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3700	0.18998960192043543	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2151	0.11045071181915044	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2048	0.105161812090014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0539416423830857E-4
2	0.0	0.0	0.0	0.0	2.0539416423830857E-4
3	0.0	0.0	0.0	0.0	2.0539416423830857E-4
4	0.0	0.0	0.0	0.0	2.0539416423830857E-4
5	0.0	0.0	0.0	0.0	2.567427052978857E-4
6	0.0	0.0	0.0	0.0	2.567427052978857E-4
7	0.0	0.0	0.0	0.0	2.567427052978857E-4
8	0.0	0.0	0.0	0.0	2.567427052978857E-4
9	0.0	0.0	0.0	5.1348541059577143E-5	2.567427052978857E-4
10	0.0	0.0	0.0	5.1348541059577143E-5	3.080912463574629E-4
11	0.0	0.0	0.0	5.1348541059577143E-5	3.5943978741704E-4
12	0.0	0.0	0.0	5.1348541059577143E-5	9.242737390723886E-4
13	0.0	0.0	0.0	5.1348541059577143E-5	0.0011296679033106973
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	13.204176	1
TTGCGCG	65	8.0435164E-4	11.689099	18
CTATACG	60	0.0058919573	11.079438	4
ACCGATA	80	3.7775838E-4	10.685666	15
GTATTAC	120	1.5228052E-6	10.292279	1
TTTGCGC	75	0.0026524242	10.131333	17
GTAATAC	85	6.611198E-4	10.056063	3
GTATAGG	190	1.0004442E-10	10.000595	1
GACGACA	95	1.6467315E-4	9.99854	13
ACTATCC	95	1.6492943E-4	9.996999	8
GTATCAA	3030	0.0	9.876825	1
GTCCTAA	145	1.6158265E-7	9.828171	1
TAGGACA	245	0.0	9.690471	4
GTTCTAA	200	2.6375346E-10	9.500566	1
GTCTATC	80	0.0045140963	9.500566	1
ATAGGAC	195	1.6898412E-9	9.25387	3
GTGCTAT	230	4.1836756E-11	9.087497	1
CTATACC	115	1.1149307E-4	9.083763	4
AGGGTCG	105	4.507402E-4	9.044671	5
CCGTCCA	95	0.0018288767	8.997299	9
>>END_MODULE