FastQCFastQC Report
Mon 15 Aug 2016
SRR2921165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947475
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59310.30454819702435204No Hit
GGTATCAACGCAGAGTACTTTTTTT42350.21746107138730922No Hit
TATCAACGCAGAGTACTTTTTTTTT40660.20878316794824067No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29630.15214572715952707No Hit
CTTATACACATCTCCGAGCCCACGA24090.12369863541252134No Hit
GGTATCAACGCAGAGTACATGGGGG22350.11476398926815491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC250.00605517218.9877247
CTTATAC3200.014.5674041
CCCTACG551.9372375E-413.8376831
GGTATCA17000.013.4866521
TATACAC4050.012.9078153
TAGACCG550.003070789412.0899295
GTCCTAT1657.2759576E-1211.5314021
TTATACA4000.010.93057252
ACTATCT1106.056156E-610.3622648
TAGGACA2350.010.1115384
GTATTAT1606.031769E-910.1079941
CTATTAG951.6210714E-410.0141121
GATCTCG1151.0160053E-59.92167219
GTATCAA33300.09.7990941
GTATAAT800.00446407549.5134071
TAGGACT2106.548362E-119.5048474
CTAGGAC2106.548362E-119.5048473
GTAGGAC1002.7415602E-49.5048463
TAGACAG2252.7284841E-119.2881365
TAATCTG1451.7080984E-69.1716695
Produced by FastQC (version 0.11.5)