##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1947475
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14103133544718	31.0	30.0	33.0	25.0	34.0
2	29.416890589096138	31.0	30.0	34.0	25.0	34.0
3	29.55918355819715	31.0	30.0	34.0	25.0	34.0
4	33.2105064249862	35.0	35.0	37.0	28.0	37.0
5	32.67927341814401	35.0	33.0	37.0	25.0	37.0
6	32.59621253161144	35.0	33.0	37.0	25.0	37.0
7	32.43594603268335	35.0	33.0	37.0	23.0	37.0
8	32.36469222968202	35.0	33.0	37.0	23.0	37.0
9	33.70752384497875	38.0	34.0	39.0	22.0	39.0
10	33.58845222660111	38.0	34.0	39.0	20.0	39.0
11	33.583651138012044	38.0	34.0	39.0	21.0	39.0
12	33.45382559468029	38.0	34.0	39.0	18.0	39.0
13	33.418042850357516	38.0	34.0	39.0	18.0	39.0
14	34.33187640406167	39.0	34.0	41.0	17.0	41.0
15	34.30343804156664	39.0	34.0	41.0	17.0	41.0
16	34.2856796621266	39.0	34.0	41.0	17.0	41.0
17	34.26493125714066	39.0	34.0	41.0	17.0	41.0
18	34.21271184482471	39.0	34.0	41.0	17.0	41.0
19	34.24783424690946	39.0	34.0	41.0	18.0	41.0
20	34.176516771717225	39.0	34.0	41.0	16.0	41.0
21	34.10328707685593	39.0	34.0	41.0	11.0	41.0
22	34.011058935288	39.0	34.0	41.0	10.0	41.0
23	33.876813823027256	39.0	34.0	41.0	2.0	41.0
24	33.788601137370186	39.0	34.0	41.0	2.0	41.0
25	33.6438578158898	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80065.0
3	1.0
4	1308.0
5	11018.0
6	12300.0
7	9216.0
8	6966.0
9	6055.0
10	5384.0
11	4861.0
12	4548.0
13	4289.0
14	4119.0
15	4121.0
16	4210.0
17	4320.0
18	4858.0
19	5726.0
20	7142.0
21	8965.0
22	11051.0
23	13634.0
24	15802.0
25	18047.0
26	20042.0
27	22220.0
28	25597.0
29	30474.0
30	38445.0
31	48597.0
32	61511.0
33	78891.0
34	103355.0
35	135773.0
36	185071.0
37	277302.0
38	672191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.246652905475415	19.078078998009836	9.442024128686327	31.233243967828418
2	14.889587799586646	21.3966803168205	34.72583730214765	28.987894581445207
3	17.859322347332952	24.04726925289718	28.459151804060163	29.634256595709708
4	13.296368719963256	16.790315901531365	32.450988149197016	37.462327229308364
5	14.473169676418493	37.76126466614904	32.26519388118473	15.500371776247738
6	33.84353793898908	34.98411612636846	16.220911661423397	14.95143427321906
7	28.934551028425155	32.67816750068682	19.443872455254326	18.943409015633705
8	27.494539771977383	34.696597223898365	18.763926598512885	19.044936405611363
9	26.699721586923726	16.06025471048018	19.060717876461002	38.17930582613509
10	17.03112243064078	28.985298874950576	30.534642382167632	23.44893631224101
11	35.182245594394104	23.065628955929817	21.674425531587097	20.07769991808898
12	23.114221924628442	25.43851721616537	28.402205525933105	23.045055333273083
13	28.454485466108988	21.50497215439483	25.32083426408777	24.719708115408416
14	22.64976126263371	21.306430058146795	25.461739594642296	30.582069084577203
15	24.368572215123038	28.404851321201264	22.835765088730653	24.39081137494504
16	24.255622023759088	27.601028502585894	24.369215379061334	23.774134094593684
17	22.851176077011516	27.714409979414096	25.86029210521046	23.574121838363933
18	23.099740604687398	27.059589024563724	26.661175079767524	23.179495290981354
19	24.180074678487593	26.418375160030834	26.71807510061465	22.683475060866922
20	24.4498281587543	25.834109139345713	26.446555138882545	23.269507563017445
21	24.817969984628178	25.44655582313724	26.299581576205057	23.43589261602953
22	24.539916679461378	25.486862354737216	26.08370360975219	23.88951735604921
23	24.162350420634993	25.346968665234936	26.316724644558036	24.17395626957203
24	23.911531820791645	26.0076849361078	26.26985814400884	23.810925099091715
25	23.932647380148506	25.70925378416135	26.409609105442154	23.948489730247985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1646.0
1	1646.0
2	1772.0
3	1898.0
4	1898.0
5	1898.0
6	2180.0
7	2462.0
8	2462.0
9	2462.0
10	3130.5
11	3799.0
12	3799.0
13	3799.0
14	5192.5
15	6586.0
16	6586.0
17	6586.0
18	11131.0
19	15676.0
20	15676.0
21	15676.0
22	23784.0
23	31892.0
24	31892.0
25	31892.0
26	45667.5
27	59443.0
28	59443.0
29	59443.0
30	75917.0
31	92391.0
32	92391.0
33	92391.0
34	112233.0
35	132075.0
36	132075.0
37	132075.0
38	154532.0
39	176989.0
40	176989.0
41	176989.0
42	196220.0
43	215451.0
44	215451.0
45	215451.0
46	234524.5
47	253598.0
48	253598.0
49	253598.0
50	260763.0
51	267928.0
52	267928.0
53	267928.0
54	254387.0
55	240846.0
56	240846.0
57	240846.0
58	213888.5
59	186931.0
60	186931.0
61	186931.0
62	157914.0
63	128897.0
64	128897.0
65	128897.0
66	100476.5
67	72056.0
68	72056.0
69	72056.0
70	54044.0
71	36032.0
72	36032.0
73	36032.0
74	24725.0
75	13418.0
76	13418.0
77	13418.0
78	9094.5
79	4771.0
80	4771.0
81	4771.0
82	3300.5
83	1830.0
84	1830.0
85	1830.0
86	1230.5
87	631.0
88	631.0
89	631.0
90	416.5
91	202.0
92	202.0
93	202.0
94	114.0
95	26.0
96	26.0
97	26.0
98	13.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09792166780061362
2	0.0
3	0.03876814849998074
4	0.05694553203507106
5	0.07250413997612293
6	0.011142633409928241
7	0.004107883284766171
8	0.010885890704630355
9	4.6213686953619433E-4
10	2.567427052978857E-4
11	0.012323649854298515
12	1.5404562317873144E-4
13	0.006675310337745029
14	0.06131015802513511
15	0.02356898034634591
16	0.00914004030860473
17	0.0013350620675490058
18	0.013042529429132595
19	0.009961616965557965
20	0.0019512445602639316
21	0.020847507670188323
22	0.00287551829933632
23	0.009294085931783463
24	0.014839728366217796
25	0.17042580777673655
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1947475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.127945090617146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.495746853452694	21.602192187745782
2	13.574367612859126	9.536792802887343
3	7.150028485551909	7.534974241104478
4	4.464864899047383	6.2736611604304136
5	3.0202406410908473	5.304742370034481
6	2.115923896123351	4.459683506336747
7	1.5560780388292312	3.8263276733295917
8	1.1467421825714594	3.222615713797175
9	0.9121031121139491	2.8836277235387837
>10	4.337012938972837	25.35419120254171
>50	0.1560281898677812	3.7360250961092465
>100	0.06539013643188733	3.881871095279168
>500	0.003993820361105318	1.0065885088371118
>1k	0.0013312734537017726	1.0685260218731347
>5k	1.479192726335303E-4	0.3081806961549097
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5931	0.30454819702435204	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4235	0.21746107138730922	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4066	0.20878316794824067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2963	0.15214572715952707	No Hit
CTTATACACATCTCCGAGCCCACGA	2409	0.12369863541252134	No Hit
GGTATCAACGCAGAGTACATGGGGG	2235	0.11476398926815491	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0539416423830857E-4
2	0.0	0.0	0.0	0.0	2.0539416423830857E-4
3	0.0	0.0	0.0	0.0	2.0539416423830857E-4
4	0.0	0.0	0.0	0.0	2.0539416423830857E-4
5	0.0	0.0	0.0	0.0	2.567427052978857E-4
6	0.0	0.0	0.0	0.0	3.5943978741704E-4
7	0.0	0.0	0.0	0.0	3.5943978741704E-4
8	0.0	0.0	0.0	0.0	4.1078832847661715E-4
9	0.0	0.0	0.0	5.1348541059577143E-5	4.1078832847661715E-4
10	0.0	0.0	0.0	5.1348541059577143E-5	4.1078832847661715E-4
11	0.0	0.0	0.0	5.1348541059577143E-5	4.1078832847661715E-4
12	0.0	0.0	0.0	5.1348541059577143E-5	9.756222801319658E-4
13	0.0	0.0	0.0	5.1348541059577143E-5	0.0011810164443702743
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGAC	25	0.006055172	18.987724	7
CTTATAC	320	0.0	14.567404	1
CCCTACG	55	1.9372375E-4	13.837683	1
GGTATCA	1700	0.0	13.486652	1
TATACAC	405	0.0	12.907815	3
TAGACCG	55	0.0030707894	12.089929	5
GTCCTAT	165	7.2759576E-12	11.531402	1
TTATACA	400	0.0	10.9305725	2
ACTATCT	110	6.056156E-6	10.362264	8
TAGGACA	235	0.0	10.111538	4
GTATTAT	160	6.031769E-9	10.107994	1
CTATTAG	95	1.6210714E-4	10.014112	1
GATCTCG	115	1.0160053E-5	9.921672	19
GTATCAA	3330	0.0	9.799094	1
GTATAAT	80	0.0044640754	9.513407	1
TAGGACT	210	6.548362E-11	9.504847	4
CTAGGAC	210	6.548362E-11	9.504847	3
GTAGGAC	100	2.7415602E-4	9.504846	3
TAGACAG	225	2.7284841E-11	9.288136	5
TAATCTG	145	1.7080984E-6	9.171669	5
>>END_MODULE