FastQCFastQC Report
Mon 15 Aug 2016
SRR2921164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467182
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30210.122447391396338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT751.4858728E-512.6613036
GGTATCA15500.011.9545471
CGCGCCG700.001494438710.85496517
TGATCGC803.7807837E-410.68492210
TAAGGCG803.7878723E-410.6827565
TGACCCG1252.2554377E-710.6352785
ACTTCGA1303.8587314E-710.22788718
ACAACCG750.00265828310.1288375
GTATCAA31150.010.0971811
CTAACAC2600.09.4965543
ACCGTGC1502.663619E-79.4965548
CACCGTT1401.0646254E-69.4963627
AACGCAT1401.0652147E-69.4959766
TTCGACT800.0045328499.49597554
TAGACAG2650.09.3166195
GTGCGCC2054.2382453E-109.26699711
GTTCACG1151.10802415E-49.0891881
TAAATCA2106.730261E-109.0437874
CGCGACC950.00182825588.9978310
ACTGATC1804.2455213E-88.9689678
Produced by FastQC (version 0.11.5)