##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921164_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2467182
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.531852939912824	31.0	28.0	31.0	15.0	34.0
2	27.10639385339225	31.0	27.0	31.0	10.0	34.0
3	26.98270699121508	31.0	27.0	31.0	10.0	34.0
4	30.861568785764486	35.0	32.0	37.0	16.0	37.0
5	30.548269239966892	35.0	31.0	37.0	10.0	37.0
6	30.84535271414918	35.0	32.0	37.0	11.0	37.0
7	30.867658324355478	35.0	32.0	37.0	10.0	37.0
8	30.546347614403803	35.0	31.0	37.0	10.0	37.0
9	32.174121325463624	37.0	32.0	39.0	10.0	39.0
10	31.5622005186484	37.0	31.0	39.0	8.0	39.0
11	32.00315744845739	37.0	32.0	39.0	8.0	39.0
12	31.70839686735717	37.0	31.0	39.0	7.0	39.0
13	31.70776051381698	37.0	32.0	39.0	2.0	39.0
14	32.82780354266527	38.0	32.0	40.0	2.0	41.0
15	32.470243378883275	38.0	31.0	40.0	2.0	41.0
16	32.3929843846137	38.0	31.0	40.0	2.0	41.0
17	32.72284574060608	38.0	32.0	40.0	2.0	41.0
18	32.78460081177635	38.0	32.0	40.0	2.0	41.0
19	32.4836728705057	38.0	32.0	40.0	2.0	41.0
20	32.364781763161375	38.0	32.0	40.0	2.0	41.0
21	32.31513686464963	38.0	32.0	40.0	2.0	41.0
22	31.54325825982842	38.0	30.0	40.0	2.0	41.0
23	31.64843898828704	38.0	31.0	40.0	2.0	41.0
24	31.280209567028294	38.0	30.0	40.0	2.0	41.0
25	30.787053002170087	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	186133.0
3	6.0
4	1379.0
5	8453.0
6	10409.0
7	9073.0
8	7796.0
9	7142.0
10	6806.0
11	6561.0
12	6357.0
13	6225.0
14	6163.0
15	6444.0
16	6806.0
17	7523.0
18	8628.0
19	10727.0
20	13408.0
21	16856.0
22	20466.0
23	24164.0
24	27948.0
25	31944.0
26	35989.0
27	40785.0
28	45357.0
29	53721.0
30	63339.0
31	75330.0
32	94504.0
33	120511.0
34	155260.0
35	201399.0
36	266338.0
37	353683.0
38	523549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76648644046149	17.8090250958402	12.087035674246708	25.337452789451596
2	15.893771977243226	20.182261832683604	38.64661343126636	25.277352758806813
3	18.61249481112495	23.769101546284766	30.314421505292653	27.303982137297634
4	13.734181679326188	15.851925357875698	35.11596250868493	35.29793045411319
5	13.861592430478819	36.851799755649296	34.09045029765549	15.196157516216408
6	33.48853158298567	34.34773568226229	17.205442517673106	14.95829021707894
7	29.211390428152367	31.60603448031263	20.761310191890118	18.421264899644886
8	26.255128920688232	34.524795339699416	20.535624896388	18.684450843224354
9	27.0621759767818	15.666976080550295	19.20171218023291	38.06913576243499
10	15.729000396413953	28.280244398524918	33.022336183352394	22.96841902170873
11	35.39679052301147	22.850579502412703	21.963206298967467	19.789423675608365
12	23.085488582916387	24.937970239497098	29.40382924578054	22.572711931805976
13	28.494364970273356	21.541204850142623	25.301755710121572	24.66267446946245
14	22.636698441525276	21.152569993534225	25.95714751898688	30.25358404595362
15	24.63719415554462	28.12455665170396	23.041424032700153	24.19682516005127
16	24.110447580862594	27.271606775279484	24.913184484339148	23.70476115951877
17	23.24258331028901	27.424215269910494	25.743331405990116	23.589870013810373
18	23.223705654466052	26.990998324854903	26.47888717176156	23.30640884891748
19	24.207933114261785	26.474902038046455	26.723161505675026	22.594003342016737
20	24.73855688508421	25.924957505485875	26.165796912504476	23.17068869692544
21	25.25419317383894	25.64561822861007	25.786012321274228	23.31417627627676
22	24.074748898778413	25.086531067885996	27.07762725508739	23.7610927782482
23	24.642350703461403	25.440266385384803	25.869121234699826	24.048261676453965
24	24.1236648691289	25.763581963222563	26.25959050514149	23.85316266250705
25	23.709451164267957	25.57806204407055	26.81930992920941	23.89317686245208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1406.0
1	1406.0
2	1508.5
3	1611.0
4	1611.0
5	1611.0
6	1862.5
7	2114.0
8	2114.0
9	2114.0
10	2832.0
11	3550.0
12	3550.0
13	3550.0
14	5809.0
15	8068.0
16	8068.0
17	8068.0
18	13841.5
19	19615.0
20	19615.0
21	19615.0
22	30516.0
23	41417.0
24	41417.0
25	41417.0
26	60633.5
27	79850.0
28	79850.0
29	79850.0
30	103466.0
31	127082.0
32	127082.0
33	127082.0
34	154298.5
35	181515.0
36	181515.0
37	181515.0
38	209476.5
39	237438.0
40	237438.0
41	237438.0
42	261787.5
43	286137.0
44	286137.0
45	286137.0
46	307606.5
47	329076.0
48	329076.0
49	329076.0
50	331918.5
51	334761.0
52	334761.0
53	334761.0
54	312965.0
55	291169.0
56	291169.0
57	291169.0
58	256620.5
59	222072.0
60	222072.0
61	222072.0
62	185764.5
63	149457.0
64	149457.0
65	149457.0
66	116183.0
67	82909.0
68	82909.0
69	82909.0
70	61660.0
71	40411.0
72	40411.0
73	40411.0
74	28497.5
75	16584.0
76	16584.0
77	16584.0
78	11682.5
79	6781.0
80	6781.0
81	6781.0
82	4786.0
83	2791.0
84	2791.0
85	2791.0
86	1861.5
87	932.0
88	932.0
89	932.0
90	655.5
91	379.0
92	379.0
93	379.0
94	212.0
95	45.0
96	45.0
97	45.0
98	28.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03392534478607577
2	0.0518405208857717
3	0.014834738580291197
4	0.010700467172669061
5	0.00956556913920416
6	6.079810893561967E-4
7	0.003769482754008419
8	0.0018239432680685899
9	0.00616087503880946
10	0.0025940526479197724
11	0.009646633284451653
12	0.008957588049847963
13	0.008228010742620528
14	0.014064629200440017
15	0.00648513161979943
16	0.0301963941046911
17	0.037289506813846726
18	0.021481998490585616
19	0.01653708563048855
20	0.015929104541132354
21	0.02245476823355553
22	0.0060392788209382205
23	0.015402187597023649
24	0.011713768988262723
25	0.4599174280616509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2467182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.48067289998177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.8758582787905	31.32520381083172
2	12.707513858390469	11.558925623308841
3	6.1145597645181065	8.342828777323126
4	3.523708371554374	6.410425113663674
5	2.2635061285770233	5.147289092045783
6	1.508607029817908	4.116747771465679
7	1.0534235972237627	3.3537289835319264
8	0.7673433088349985	2.7919432024891404
9	0.5554145553027374	2.2734564942251367
>10	2.509249700146333	18.776120195744266
>50	0.08747979596083306	2.709142742872358
>100	0.03148643421177354	2.317847443686174
>500	0.0012019648361816732	0.38850826191355736
>1k	6.472118348663502E-4	0.48783248689854136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3021	0.122447391396338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.0532072623746444E-5	0.0	0.0	0.0
6	0.0	4.0532072623746444E-5	0.0	0.0	8.106414524749289E-5
7	0.0	4.0532072623746444E-5	0.0	0.0	8.106414524749289E-5
8	0.0	4.0532072623746444E-5	0.0	0.0	1.2159621787123934E-4
9	0.0	8.106414524749289E-5	0.0	0.0	1.6212829049498578E-4
10	0.0	8.106414524749289E-5	0.0	0.0	1.6212829049498578E-4
11	0.0	8.106414524749289E-5	0.0	4.0532072623746444E-5	1.6212829049498578E-4
12	0.0	1.2159621787123934E-4	0.0	4.0532072623746444E-5	1.6212829049498578E-4
13	0.0	1.2159621787123934E-4	0.0	4.0532072623746444E-5	1.6212829049498578E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGT	75	1.4858728E-5	12.661303	6
GGTATCA	1550	0.0	11.954547	1
CGCGCCG	70	0.0014944387	10.854965	17
TGATCGC	80	3.7807837E-4	10.684922	10
TAAGGCG	80	3.7878723E-4	10.682756	5
TGACCCG	125	2.2554377E-7	10.635278	5
ACTTCGA	130	3.8587314E-7	10.227887	18
ACAACCG	75	0.002658283	10.128837	5
GTATCAA	3115	0.0	10.097181	1
CTAACAC	260	0.0	9.496554	3
ACCGTGC	150	2.663619E-7	9.496554	8
CACCGTT	140	1.0646254E-6	9.496362	7
AACGCAT	140	1.0652147E-6	9.495976	6
TTCGACT	80	0.004532849	9.4959755	4
TAGACAG	265	0.0	9.316619	5
GTGCGCC	205	4.2382453E-10	9.266997	11
GTTCACG	115	1.10802415E-4	9.089188	1
TAAATCA	210	6.730261E-10	9.043787	4
CGCGACC	95	0.0018282558	8.99783	10
ACTGATC	180	4.2455213E-8	8.968967	8
>>END_MODULE