FastQCFastQC Report
Mon 15 Aug 2016
SRR2921164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467182
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57200.23184345540782966No Hit
TATCAACGCAGAGTACTTTTTTTTT40920.16585724117637046No Hit
GGTATCAACGCAGAGTACTTTTTTT40800.1653708563048855No Hit
GGTATCAACGCAGAGTACATGGGGG25400.10295146446431597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.013.154211
CCGATCA802.8674252E-511.8752169
ATTAACG600.005856145611.0887082
TAAGCCG600.00585791511.0882574
GGTTTAG700.001475136110.8718941
TAAGGCG803.7780986E-410.6857435
TCTTATA1451.43372745E-810.4878422
CCCTACG1002.3642464E-510.4641981
GACCGTG2450.09.6871777
CGTGTAC900.0011090739.5046063
ACCGATA1201.7048436E-59.4982468
ACCGTCC1304.2504744E-69.4982458
ATAGGAC2451.8189894E-129.3106353
GTATCAA38300.09.2893661
TGAACTG3100.09.1920385
GTGCTAT2900.09.18487551
AGACCGT2900.09.1660746
TATAAGT2600.09.1386744
TGTACCG2401.0913936E-119.1026715
TGGACCG1151.1129816E-49.0854635
Produced by FastQC (version 0.11.5)