##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2467182
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.80248234625577	31.0	30.0	33.0	26.0	34.0
2	30.060796487652716	31.0	30.0	34.0	26.0	34.0
3	30.185529887945034	31.0	30.0	34.0	26.0	34.0
4	33.882944590224795	35.0	35.0	37.0	30.0	37.0
5	33.44884244453794	35.0	35.0	37.0	28.0	37.0
6	33.406558575735396	36.0	35.0	37.0	28.0	37.0
7	33.27029380078162	36.0	35.0	37.0	27.0	37.0
8	33.220301137086764	37.0	35.0	37.0	27.0	37.0
9	34.65163534753415	39.0	35.0	39.0	27.0	39.0
10	34.54550171004814	39.0	35.0	39.0	27.0	39.0
11	34.548323147623485	39.0	35.0	39.0	27.0	39.0
12	34.42680718325604	38.0	35.0	39.0	27.0	39.0
13	34.41131501445779	38.0	35.0	39.0	27.0	39.0
14	35.37434408973477	39.0	35.0	41.0	26.0	41.0
15	35.36136612540137	39.0	35.0	41.0	26.0	41.0
16	35.34657678274242	39.0	35.0	41.0	26.0	41.0
17	35.326370733898024	39.0	35.0	41.0	26.0	41.0
18	35.283621556901764	39.0	35.0	41.0	26.0	41.0
19	35.33185026479603	39.0	35.0	41.0	26.0	41.0
20	35.273627158434195	39.0	35.0	41.0	25.0	41.0
21	35.206309465617046	39.0	35.0	41.0	25.0	41.0
22	35.12641183341967	39.0	35.0	41.0	25.0	41.0
23	35.00395066111864	39.0	35.0	41.0	25.0	41.0
24	34.92134670243217	39.0	35.0	41.0	24.0	41.0
25	34.77984842626122	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69352.0
3	2.0
4	1110.0
5	9611.0
6	10815.0
7	8068.0
8	6313.0
9	5657.0
10	5048.0
11	4566.0
12	4447.0
13	4121.0
14	3990.0
15	4002.0
16	4049.0
17	4323.0
18	4907.0
19	5622.0
20	7181.0
21	9417.0
22	11922.0
23	14308.0
24	17338.0
25	19766.0
26	22986.0
27	25979.0
28	30066.0
29	36792.0
30	46515.0
31	59693.0
32	77537.0
33	100558.0
34	132960.0
35	178401.0
36	242912.0
37	369182.0
38	907666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.58928847457586	18.944881193344866	9.36423722301539	31.10159310906389
2	15.007891594539844	21.159768513226833	34.986150190784464	28.84618970144886
3	17.937045172272434	24.037448995025102	28.35740766796922	29.668098164733248
4	13.336721008710759	16.42026262459947	33.43911369976734	36.80390266692243
5	14.517696455355091	37.61446866018636	32.66111598274118	15.206718901717364
6	33.91080068924787	35.090718136777035	16.399186554559172	14.599294619415923
7	29.60500236304665	32.006068530784866	19.649644894481657	18.739284211686826
8	27.669576080572376	34.65289907091428	18.770279795820837	18.90724505269251
9	27.122010297599243	15.418350608813231	18.95084949316567	38.50878960042186
10	16.57979278349596	28.722045997491875	31.310023030361005	23.388138188651165
11	35.798681413113314	22.53441884691543	21.612988871256388	20.053910868714876
12	23.427070582608483	24.952942225536848	28.76989113076468	22.85009606108999
13	28.858082988668627	21.091534624008766	25.178952727296313	24.871429660026298
14	22.735090568800047	20.914143656939622	25.758322332868982	30.59244344139135
15	24.82237157361394	28.037237307361433	22.627911521057168	24.51247959796746
16	24.55722144014955	27.12684696267791	24.309318140271273	24.006613456901267
17	23.35715684077026	27.17290370225655	25.783012203045512	23.686927253927674
18	23.401837034154568	26.603432481293222	26.59670371541374	23.398026769138475
19	24.377810790598506	26.142992353230877	26.587895284053253	22.891301572117364
20	24.790417511258863	25.6891811539308	26.176622822857148	23.343778511953182
21	25.148055525940173	25.299343970819006	26.03190921396086	23.520691289279966
22	24.842349225547412	25.127332538357837	25.764988273976336	24.265329962118418
23	24.47864754186405	25.241529200561146	25.929018484507882	24.350804773066926
24	24.22860899921037	25.61845047288741	26.113267600929397	24.039672926972823
25	24.215197659712253	25.462160137558897	26.075531528312652	24.2471106744162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	789.0
1	789.0
2	854.5
3	920.0
4	920.0
5	920.0
6	1368.5
7	1817.0
8	1817.0
9	1817.0
10	2395.5
11	2974.0
12	2974.0
13	2974.0
14	4366.5
15	5759.0
16	5759.0
17	5759.0
18	11081.5
19	16404.0
20	16404.0
21	16404.0
22	26476.0
23	36548.0
24	36548.0
25	36548.0
26	55347.5
27	74147.0
28	74147.0
29	74147.0
30	95858.0
31	117569.0
32	117569.0
33	117569.0
34	143055.5
35	168542.0
36	168542.0
37	168542.0
38	196682.0
39	224822.0
40	224822.0
41	224822.0
42	249916.0
43	275010.0
44	275010.0
45	275010.0
46	299206.5
47	323403.0
48	323403.0
49	323403.0
50	333612.0
51	343821.0
52	343821.0
53	343821.0
54	324693.0
55	305565.0
56	305565.0
57	305565.0
58	270850.0
59	236135.0
60	236135.0
61	236135.0
62	200371.0
63	164607.0
64	164607.0
65	164607.0
66	128348.0
67	92089.0
68	92089.0
69	92089.0
70	68547.5
71	45006.0
72	45006.0
73	45006.0
74	31748.0
75	18490.0
76	18490.0
77	18490.0
78	13095.5
79	7701.0
80	7701.0
81	7701.0
82	5513.0
83	3325.0
84	3325.0
85	3325.0
86	2245.0
87	1165.0
88	1165.0
89	1165.0
90	839.0
91	513.0
92	513.0
93	513.0
94	286.5
95	60.0
96	60.0
97	60.0
98	30.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09642580077189279
2	0.0
3	0.04057260469637019
4	0.05597479229339383
5	0.0672832405554191
6	0.005877150530443235
7	0.0011348980334649006
8	0.005390765658958277
9	2.026603631187322E-4
10	1.6212829049498578E-4
11	0.005958214675690727
12	8.106414524749289E-5
13	0.002026603631187322
14	0.05581266400289885
15	0.020914549473853166
16	0.0036884186087609264
17	4.458527988612109E-4
18	0.006566195765046924
19	0.005147573223215798
20	6.079810893561967E-4
21	0.01503739894340993
22	8.106414524749288E-4
23	0.0036073544635134335
24	0.008511735250986753
25	0.17173439170681368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2467182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.3580904533146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.9674246677276	19.33682595411757
2	14.784003884988376	9.863322777151936
3	7.98203791432526	7.98796628243546
4	4.917793712112506	6.561928299175632
5	3.287479166987834	5.483201370788376
6	2.282626798129773	4.5686442721903715
7	1.6583921405959121	3.8724556523145126
8	1.2514593629608997	3.3397035702637723
9	0.9371058188945784	2.8134054603911522
>10	4.670117189686176	26.249601346462548
>50	0.18522109757337007	4.206687804788534
>100	0.07278286242672317	4.083164988549863
>500	0.0020841903808809783	0.48683586605206325
>1k	0.0013485937758640483	0.9123264868858139
>5k	1.225994341694589E-4	0.23392986843226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5720	0.23184345540782966	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4092	0.16585724117637046	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4080	0.1653708563048855	No Hit
GGTATCAACGCAGAGTACATGGGGG	2540	0.10295146446431597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0532072623746444E-5
2	0.0	0.0	0.0	0.0	8.106414524749289E-5
3	0.0	0.0	0.0	0.0	8.106414524749289E-5
4	0.0	0.0	0.0	0.0	1.2159621787123934E-4
5	0.0	4.0532072623746444E-5	0.0	0.0	1.2159621787123934E-4
6	0.0	4.0532072623746444E-5	0.0	0.0	2.4319243574247868E-4
7	0.0	4.0532072623746444E-5	0.0	0.0	2.4319243574247868E-4
8	0.0	4.0532072623746444E-5	0.0	0.0	3.2425658098997155E-4
9	0.0	4.0532072623746444E-5	0.0	0.0	4.0532072623746445E-4
10	0.0	4.0532072623746444E-5	0.0	0.0	4.458527988612109E-4
11	0.0	4.0532072623746444E-5	0.0	4.0532072623746444E-5	4.458527988612109E-4
12	0.0	4.0532072623746444E-5	0.0	4.0532072623746444E-5	4.458527988612109E-4
13	0.0	4.0532072623746444E-5	0.0	4.0532072623746444E-5	4.458527988612109E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2090	0.0	13.15421	1
CCGATCA	80	2.8674252E-5	11.875216	9
ATTAACG	60	0.0058561456	11.088708	2
TAAGCCG	60	0.005857915	11.088257	4
GGTTTAG	70	0.0014751361	10.871894	1
TAAGGCG	80	3.7780986E-4	10.685743	5
TCTTATA	145	1.43372745E-8	10.487842	2
CCCTACG	100	2.3642464E-5	10.464198	1
GACCGTG	245	0.0	9.687177	7
CGTGTAC	90	0.001109073	9.504606	3
ACCGATA	120	1.7048436E-5	9.498246	8
ACCGTCC	130	4.2504744E-6	9.498245	8
ATAGGAC	245	1.8189894E-12	9.310635	3
GTATCAA	3830	0.0	9.289366	1
TGAACTG	310	0.0	9.192038	5
GTGCTAT	290	0.0	9.1848755	1
AGACCGT	290	0.0	9.166074	6
TATAAGT	260	0.0	9.138674	4
TGTACCG	240	1.0913936E-11	9.102671	5
TGGACCG	115	1.1129816E-4	9.085463	5
>>END_MODULE