FastQCFastQC Report
Mon 15 Aug 2016
SRR2921163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229311
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA400.00527485214.2523941
GGTATCA14250.012.6687951
GTATCAA31600.010.8546711
TTAGTAC1408.411007E-910.853253
TATACCG951.6518083E-49.9959495
TATACTG4250.09.8313115
GTTCTAG2900.09.8292371
GTATAAC2600.09.50159551
CGCACGA900.00111580249.49850612
CGGAAAT1201.7070008E-59.49747610
CGATCAA800.0045275899.49747610
TGGACCG3000.09.4961525
AATACCG1002.7681421E-49.4961525
TAGGACT3600.09.2325124
GTTCTAA3100.09.1950931
AGGACTG5800.09.1686985
GAATCGA1252.7695794E-59.1161646
CTAGACA2304.1836756E-119.0834174
GTCCTAG4200.09.0491391
TTCTAGG2750.08.9817962
Produced by FastQC (version 0.11.5)