##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921163_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3229311
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.302582811008293	31.0	28.0	31.0	12.0	34.0
2	27.062821450148345	31.0	27.0	31.0	10.0	34.0
3	26.835640481824143	31.0	27.0	31.0	10.0	34.0
4	30.703712649540414	35.0	31.0	37.0	16.0	37.0
5	30.493683327496175	35.0	30.0	37.0	10.0	37.0
6	31.04387870973096	35.0	32.0	37.0	11.0	37.0
7	31.041030424136913	35.0	32.0	37.0	11.0	37.0
8	30.668424317137617	35.0	31.0	37.0	10.0	37.0
9	32.351421402274354	37.0	32.0	39.0	10.0	39.0
10	31.713992551352284	37.0	31.0	39.0	10.0	39.0
11	32.17695756153557	37.0	32.0	39.0	10.0	39.0
12	31.86749433547899	37.0	32.0	39.0	8.0	39.0
13	31.878981305919435	37.0	32.0	39.0	8.0	39.0
14	33.004878749677566	38.0	32.0	40.0	2.0	41.0
15	32.66424788445585	38.0	32.0	40.0	2.0	41.0
16	32.58488079965045	38.0	32.0	40.0	2.0	41.0
17	32.90676710914495	38.0	32.0	40.0	2.0	41.0
18	32.96157601420241	38.0	32.0	40.0	2.0	41.0
19	32.6614770147564	38.0	32.0	40.0	2.0	41.0
20	32.538798214232074	38.0	32.0	40.0	2.0	41.0
21	32.49293703827225	38.0	32.0	40.0	2.0	41.0
22	31.729672056980576	38.0	30.0	40.0	2.0	41.0
23	31.843055066545155	38.0	31.0	40.0	2.0	41.0
24	31.49091307712388	38.0	30.0	40.0	2.0	41.0
25	30.988397215381237	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	240099.0
3	17.0
4	1824.0
5	10105.0
6	12584.0
7	11252.0
8	9840.0
9	9092.0
10	8659.0
11	8230.0
12	8051.0
13	7791.0
14	7834.0
15	8164.0
16	8676.0
17	9703.0
18	11265.0
19	13803.0
20	17010.0
21	21556.0
22	26211.0
23	31335.0
24	36152.0
25	41062.0
26	46753.0
27	52057.0
28	57761.0
29	68547.0
30	81247.0
31	97225.0
32	122247.0
33	155432.0
34	201653.0
35	263930.0
36	355435.0
37	485278.0
38	681431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.768535598863394	17.651501282290756	11.823250491681605	25.75671262716424
2	16.168377272463918	19.441357363918616	38.62698362781861	25.763281735798856
3	19.170849018766773	22.91197006979276	29.906142908687162	28.01103800275331
4	13.653344862304259	15.554324339641749	34.70338965991291	36.08894113814108
5	14.181091135223124	36.442816422649216	34.34625661659137	15.029835825536294
6	34.576992025179536	33.9920645070788	16.890064655099003	14.54087881264266
7	29.79807004173494	31.18306175327613	20.31695884012983	18.7019093648591
8	26.76725355252988	33.971252749866224	20.325846760608044	18.93564693699586
9	27.025325432645648	15.532073162498614	18.92036941722828	38.52223198762746
10	15.758167327716269	27.732094061454077	32.916461249941165	23.59327736088849
11	36.20203056281022	22.245077899767633	21.86492418449673	19.68796735292542
12	23.295990872806463	24.165353579648638	29.373942407507887	23.164713140037012
13	28.774397102325374	20.867729180396104	25.327276691259904	25.03059702601862
14	22.705618333869133	20.28662465753764	25.667720301707558	31.34003670688567
15	24.60876216666512	27.797690385276436	22.768843427445955	24.82470402061249
16	24.423721752167133	27.037166102135924	24.607500127774447	23.931612017922497
17	23.57003854286771	27.072910516236515	25.50421394172536	23.852836999170407
18	23.630422958891007	26.840247191651585	26.098034187081165	23.431295662376247
19	24.66461182434567	26.06497902004338	26.328018245810515	22.942390909800434
20	24.860621808997447	25.75686670455478	25.875796153685343	23.506715332762433
21	25.317802056754328	25.332700238061168	25.59303124405699	23.756466461127516
22	24.220625506328645	24.80378603759281	26.852450568698423	24.12313788738012
23	24.77179694209606	25.098480463253836	25.814997647742377	24.314724946907734
24	24.311785678006625	25.426487239140076	25.91364461853688	24.34808246431642
25	23.917060311599403	25.154074540063114	26.67187482013359	24.25699032820389
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1064.0
1	1064.0
2	1142.5
3	1221.0
4	1221.0
5	1221.0
6	1685.5
7	2150.0
8	2150.0
9	2150.0
10	2941.0
11	3732.0
12	3732.0
13	3732.0
14	6006.5
15	8281.0
16	8281.0
17	8281.0
18	14982.5
19	21684.0
20	21684.0
21	21684.0
22	34367.0
23	47050.0
24	47050.0
25	47050.0
26	69174.5
27	91299.0
28	91299.0
29	91299.0
30	121727.5
31	152156.0
32	152156.0
33	152156.0
34	187800.0
35	223444.0
36	223444.0
37	223444.0
38	263670.5
39	303897.0
40	303897.0
41	303897.0
42	340163.5
43	376430.0
44	376430.0
45	376430.0
46	404807.0
47	433184.0
48	433184.0
49	433184.0
50	437912.5
51	442641.0
52	442641.0
53	442641.0
54	418478.5
55	394316.0
56	394316.0
57	394316.0
58	348274.0
59	302232.0
60	302232.0
61	302232.0
62	254523.5
63	206815.0
64	206815.0
65	206815.0
66	163088.0
67	119361.0
68	119361.0
69	119361.0
70	89553.5
71	59746.0
72	59746.0
73	59746.0
74	42080.5
75	24415.0
76	24415.0
77	24415.0
78	16859.0
79	9303.0
80	9303.0
81	9303.0
82	6340.5
83	3378.0
84	3378.0
85	3378.0
86	2280.5
87	1183.0
88	1183.0
89	1183.0
90	731.0
91	279.0
92	279.0
93	279.0
94	160.5
95	42.0
96	42.0
97	42.0
98	25.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03418685905445465
2	0.05270474104228425
3	0.014430322753057851
4	0.010652427096677899
5	0.00950667185662824
6	6.812598724619586E-4
7	0.003777895656379952
8	0.0019508805438683359
9	0.006967430513815485
10	0.003189534857435533
11	0.010962090675069697
12	0.008639613837131202
13	0.007400959523564005
14	0.01514254898335899
15	0.006224237925675167
16	0.029913501672647818
17	0.037686057490281984
18	0.022388676717727096
19	0.01594767428717767
20	0.01566897706662505
21	0.022574474864762172
22	0.005666843484569929
23	0.01542124620391161
24	0.011612384189692475
25	0.46821133052840064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3229311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.60960296631501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32317913311137	32.31125855757055
2	12.730719640366289	11.867475758258827
3	6.099573944534086	8.528961594552413
4	3.506322986640817	6.537132891159694
5	2.1844071533624816	5.090717506750182
6	1.4293042586709968	3.997158240883102
7	0.9966103969834337	3.2516130440849604
8	0.7473709402692927	2.7867730235610613
9	0.5463826570549916	2.292001084171207
>10	2.324246022572352	17.715902035430883
>50	0.07901070321656212	2.497346047136301
>100	0.031504281800267486	2.4582345678531436
>500	8.891229211816272E-4	0.2969136002433974
>1k	4.7875849592568135E-4	0.36851204834420215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.289907351753981E-5
2	0.0	0.0	0.0	0.0	9.289907351753981E-5
3	0.0	0.0	0.0	0.0	9.289907351753981E-5
4	0.0	0.0	0.0	0.0	9.289907351753981E-5
5	0.0	0.0	0.0	0.0	9.289907351753981E-5
6	0.0	0.0	0.0	0.0	2.1676450487425955E-4
7	0.0	0.0	0.0	0.0	2.1676450487425955E-4
8	0.0	0.0	0.0	0.0	2.1676450487425955E-4
9	0.0	0.0	0.0	0.0	2.1676450487425955E-4
10	0.0	0.0	0.0	0.0	2.1676450487425955E-4
11	0.0	0.0	0.0	3.096635783917993E-5	2.1676450487425955E-4
12	0.0	0.0	0.0	3.096635783917993E-5	4.025626519093392E-4
13	0.0	0.0	0.0	3.096635783917993E-5	5.264280832660589E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAA	40	0.005274852	14.252394	1
GGTATCA	1425	0.0	12.668795	1
GTATCAA	3160	0.0	10.854671	1
TTAGTAC	140	8.411007E-9	10.85325	3
TATACCG	95	1.6518083E-4	9.995949	5
TATACTG	425	0.0	9.831311	5
GTTCTAG	290	0.0	9.829237	1
GTATAAC	260	0.0	9.5015955	1
CGCACGA	90	0.0011158024	9.498506	12
CGGAAAT	120	1.7070008E-5	9.497476	10
CGATCAA	80	0.004527589	9.497476	10
TGGACCG	300	0.0	9.496152	5
AATACCG	100	2.7681421E-4	9.496152	5
TAGGACT	360	0.0	9.232512	4
GTTCTAA	310	0.0	9.195093	1
AGGACTG	580	0.0	9.168698	5
GAATCGA	125	2.7695794E-5	9.116164	6
CTAGACA	230	4.1836756E-11	9.083417	4
GTCCTAG	420	0.0	9.049139	1
TTCTAGG	275	0.0	8.981796	2
>>END_MODULE