FastQCFastQC Report
Mon 15 Aug 2016
SRR2921163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229311
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50980.15786649226413932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.012.8289791
TGATCGC700.001492135810.85716110
TCGTACA1502.1191227E-910.7718582
TTACGAT1158.8998604E-710.7321646
GTATCAA32500.010.5389331
CGGATAG750.002651240210.1322525
GTCTTAG2750.010.0332771
CGATTCG951.6450419E-410.0000169
ACCGCGC951.6483883E-49.9980028
GTACTAG1304.1614476E-69.5143141
TAGGCCC1901.0440999E-99.5044334
ATTACGA1201.703793E-59.4989845
TACGATT1002.7749254E-49.4938387
AGAACCG2451.8189894E-129.3051285
ATTATAC3600.09.2405643
CTGATCG1651.0666918E-79.2121369
TAGGACA3200.09.2074194
TAATACT4050.09.1524174
CCGCCTA1252.7558266E-59.12001514
GTCCTAG3600.08.9857421
Produced by FastQC (version 0.11.5)