##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3229311
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.588779464102405	31.0	30.0	33.0	26.0	34.0
2	29.87043521048298	31.0	30.0	34.0	26.0	34.0
3	30.008656645333943	31.0	30.0	34.0	26.0	34.0
4	33.689542134529624	35.0	35.0	37.0	30.0	37.0
5	33.22065387941886	35.0	35.0	37.0	27.0	37.0
6	33.15413938143462	36.0	35.0	37.0	27.0	37.0
7	33.0070005645167	36.0	35.0	37.0	26.0	37.0
8	32.950747698193204	36.0	35.0	37.0	26.0	37.0
9	34.36431950964153	39.0	35.0	39.0	26.0	39.0
10	34.25153229280178	38.0	35.0	39.0	26.0	39.0
11	34.24954611060997	38.0	35.0	39.0	26.0	39.0
12	34.121578875493874	38.0	35.0	39.0	25.0	39.0
13	34.09855972373054	38.0	35.0	39.0	25.0	39.0
14	35.06050485691839	39.0	35.0	41.0	25.0	41.0
15	35.04169774914835	39.0	35.0	41.0	25.0	41.0
16	35.02491026723657	39.0	35.0	41.0	25.0	41.0
17	34.99608802001418	39.0	35.0	41.0	25.0	41.0
18	34.94449187458253	39.0	35.0	41.0	25.0	41.0
19	34.983578230774306	39.0	35.0	41.0	24.0	41.0
20	34.915779248266894	39.0	35.0	41.0	24.0	41.0
21	34.84974349017484	39.0	35.0	41.0	23.0	41.0
22	34.768737356049016	39.0	35.0	41.0	23.0	41.0
23	34.63725234268239	39.0	34.0	41.0	21.0	41.0
24	34.557830757087196	39.0	34.0	41.0	19.0	41.0
25	34.41317420341367	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103073.0
3	6.0
4	1618.0
5	14279.0
6	16087.0
7	12113.0
8	9532.0
9	8031.0
10	7268.0
11	6639.0
12	6369.0
13	5989.0
14	5816.0
15	5894.0
16	5908.0
17	6026.0
18	6816.0
19	8078.0
20	10121.0
21	13126.0
22	16532.0
23	19977.0
24	24012.0
25	27821.0
26	31539.0
27	35180.0
28	40610.0
29	49240.0
30	62505.0
31	79659.0
32	101971.0
33	131632.0
34	172048.0
35	229676.0
36	312668.0
37	472817.0
38	1168635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49537231348368	18.4865366529263	9.110209552457551	31.907881481132467
2	14.944581057693112	20.62761994741293	34.78952631072077	29.638272684173188
3	17.924996197362905	23.28301254359092	28.21668247097372	30.57530878807245
4	13.322982694334746	15.816586098581626	32.99862618040352	37.86180502668011
5	14.644062607773078	37.208913920044424	32.75316235068248	15.393861121500018
6	34.72492067729175	34.765704595607474	16.017705856595445	14.49166887050533
7	30.112975682807214	31.572837156529367	19.45332069480525	18.860866465858166
8	28.031079787578307	34.7175509261166	18.487525071711985	18.763844214593107
9	27.115533254224438	15.430848257085739	18.58601729660633	38.8676011920835
10	16.623514966842713	28.311974248328976	31.141561419562414	23.9229493652659
11	36.42032291798599	22.320291362583344	21.420203694923977	19.839182024506687
12	23.477220668570272	24.299687642155025	28.75054694363407	23.47254474564063
13	28.930400239806964	20.64204103473733	25.075001610274043	25.35255711518166
14	22.693352517700305	20.13732685427193	25.40295845741827	31.7663621706095
15	24.690780242890902	27.857034451358327	22.300711451677348	25.151473854073426
16	24.890493657225406	26.95226313108401	23.85812251394667	24.299120697743916
17	23.60030681600361	27.03102254143859	25.44872769522283	23.919942947334977
18	23.75752849983696	26.70107942174432	26.026800824593117	23.514591253825603
19	24.8195774527944	25.871222971456	25.85762837832607	23.45157119742353
20	24.791192620549644	25.687797820698776	25.744373689341195	23.776635869410384
21	25.191175855773835	25.14224573758282	25.636254120736613	24.030324285906733
22	24.98238773127038	24.97981750128434	25.37749091671433	24.66030385073095
23	24.728490680381253	25.034411876641823	25.546943683434893	24.69015375954204
24	24.413704985632748	25.41633131726278	25.577422900088226	24.592540797016245
25	24.418563176055518	25.168379017562902	25.737835846935216	24.67522195944636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	862.0
1	862.0
2	930.5
3	999.0
4	999.0
5	999.0
6	1388.5
7	1778.0
8	1778.0
9	1778.0
10	2402.0
11	3026.0
12	3026.0
13	3026.0
14	4572.5
15	6119.0
16	6119.0
17	6119.0
18	12149.5
19	18180.0
20	18180.0
21	18180.0
22	29631.0
23	41082.0
24	41082.0
25	41082.0
26	61683.0
27	82284.0
28	82284.0
29	82284.0
30	110496.5
31	138709.0
32	138709.0
33	138709.0
34	172555.5
35	206402.0
36	206402.0
37	206402.0
38	246330.5
39	286259.0
40	286259.0
41	286259.0
42	324173.0
43	362087.0
44	362087.0
45	362087.0
46	395073.5
47	428060.0
48	428060.0
49	428060.0
50	439514.0
51	450968.0
52	450968.0
53	450968.0
54	432587.5
55	414207.0
56	414207.0
57	414207.0
58	368482.5
59	322758.0
60	322758.0
61	322758.0
62	274341.5
63	225925.0
64	225925.0
65	225925.0
66	178135.5
67	130346.0
68	130346.0
69	130346.0
70	98202.5
71	66059.0
72	66059.0
73	66059.0
74	46655.5
75	27252.0
76	27252.0
77	27252.0
78	18900.5
79	10549.0
80	10549.0
81	10549.0
82	7174.5
83	3800.0
84	3800.0
85	3800.0
86	2509.0
87	1218.0
88	1218.0
89	1218.0
90	779.0
91	340.0
92	340.0
93	340.0
94	188.5
95	37.0
96	37.0
97	37.0
98	21.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10197221636441953
2	0.0
3	0.039884668896863755
4	0.05614200676243322
5	0.06329523542328379
6	0.0030037367104004536
7	9.90923450853758E-4
8	0.0022915104800993154
9	2.1676450487425957E-4
10	1.857981470350796E-4
11	0.0021676450487425956
12	6.193271567835988E-5
13	0.0014554188184414572
14	0.05211638024333983
15	0.021707416845265136
16	0.0014244524606022771
17	4.3352900974851915E-4
18	0.002539241342812755
19	0.0026631067741694748
20	5.883607989444189E-4
21	0.006688733293262866
22	8.670580194970383E-4
23	0.0014863851762806368
24	0.0028489049212045542
25	0.16059153175398713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3229311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.03423731171226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.64903820650122	21.85441835460168
2	14.658389813542247	10.564077942975338
3	7.431359839715411	8.033501520390992
4	4.541079285636571	6.54537314519716
5	2.963553917392321	5.339470257268469
6	2.043101770111382	4.417296842170375
7	1.4925120680567174	3.7647073835665132
8	1.1119742695900239	3.205531577193986
9	0.8433998623384915	2.735214370935359
>10	4.044206933804475	24.42277552803615
>50	0.15307473241173383	3.7403833188334454
>100	0.06502285717397147	4.034129021360611
>500	0.002075648669296979	0.47341749345447615
>1k	0.0011243096948390144	0.71082743730068
>5k	8.648536114146265E-5	0.15887580671484353
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5098	0.15786649226413932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2386543135671973E-4
2	0.0	0.0	0.0	0.0	1.2386543135671973E-4
3	0.0	0.0	0.0	0.0	1.2386543135671973E-4
4	0.0	0.0	0.0	0.0	1.5483178919589968E-4
5	0.0	0.0	0.0	0.0	1.8579814703507963E-4
6	0.0	0.0	0.0	0.0	2.1676450487425955E-4
7	0.0	0.0	0.0	0.0	2.4773086271343947E-4
8	0.0	0.0	0.0	0.0	2.4773086271343947E-4
9	0.0	0.0	0.0	0.0	3.406299362309793E-4
10	0.0	0.0	0.0	0.0	4.6449536758769904E-4
11	0.0	0.0	0.0	3.096635783917993E-5	4.6449536758769904E-4
12	0.0	0.0	0.0	3.096635783917993E-5	7.741589459794985E-4
13	0.0	0.0	0.0	3.096635783917993E-5	8.360916616578582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1550	0.0	12.828979	1
TGATCGC	70	0.0014921358	10.857161	10
TCGTACA	150	2.1191227E-9	10.771858	2
TTACGAT	115	8.8998604E-7	10.732164	6
GTATCAA	3250	0.0	10.538933	1
CGGATAG	75	0.0026512402	10.132252	5
GTCTTAG	275	0.0	10.033277	1
CGATTCG	95	1.6450419E-4	10.000016	9
ACCGCGC	95	1.6483883E-4	9.998002	8
GTACTAG	130	4.1614476E-6	9.514314	1
TAGGCCC	190	1.0440999E-9	9.504433	4
ATTACGA	120	1.703793E-5	9.498984	5
TACGATT	100	2.7749254E-4	9.493838	7
AGAACCG	245	1.8189894E-12	9.305128	5
ATTATAC	360	0.0	9.240564	3
CTGATCG	165	1.0666918E-7	9.212136	9
TAGGACA	320	0.0	9.207419	4
TAATACT	405	0.0	9.152417	4
CCGCCTA	125	2.7558266E-5	9.120015	14
GTCCTAG	360	0.0	8.985742	1
>>END_MODULE