FastQCFastQC Report
Mon 15 Aug 2016
SRR2921162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624864
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.013.3251971
TAATACG1302.6357156E-911.6879614
CGACCCG752.08138E-411.3963915
ACCGTGC2300.011.1481828
GCGCAAC600.00588543511.08150711
GTATCAA34200.010.5843631
GTCCTAG5050.09.783131
GAACCGT2151.0913936E-119.7170496
GTATTAG2650.09.6801881
AAGACCG3050.09.652685
GTCTAAG3250.09.6470921
TCGCGGG1401.0618533E-69.49856515
CTAATAC2451.8189894E-129.3027913
TAAGACT4100.09.2648474
TTTTACG1054.4988125E-49.0468762
GTATAGG3600.08.9730961
TAGACTG3400.08.9383455
CTAAGAC3400.08.9379763
GTCTAGA5000.08.9308691
AGAACCG2356.730261E-118.89085
Produced by FastQC (version 0.11.5)