##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921162_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3624864 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.322798041526525 31.0 28.0 31.0 10.0 34.0 2 26.913427924468337 31.0 27.0 31.0 10.0 34.0 3 26.78092860863194 31.0 26.0 31.0 10.0 34.0 4 30.68850776194638 35.0 31.0 37.0 10.0 37.0 5 30.376509573876426 35.0 30.0 37.0 10.0 37.0 6 30.72072855698862 35.0 31.0 37.0 10.0 37.0 7 30.583810592618097 35.0 31.0 37.0 10.0 37.0 8 30.262042382831467 35.0 30.0 37.0 8.0 37.0 9 31.85215086690149 37.0 32.0 39.0 8.0 39.0 10 31.21158780025954 37.0 30.0 39.0 2.0 39.0 11 31.718029421241734 37.0 32.0 39.0 2.0 39.0 12 31.413020460905567 37.0 31.0 39.0 2.0 39.0 13 31.428113992690484 37.0 31.0 39.0 2.0 39.0 14 32.52921406154824 38.0 32.0 40.0 2.0 41.0 15 32.1950823534345 38.0 31.0 40.0 2.0 41.0 16 32.11569261632988 38.0 31.0 40.0 2.0 41.0 17 32.42542975405422 38.0 32.0 40.0 2.0 41.0 18 32.480598168648534 38.0 32.0 40.0 2.0 41.0 19 32.1786094595549 38.0 31.0 40.0 2.0 41.0 20 32.055947754177815 38.0 31.0 40.0 2.0 41.0 21 32.01023017691146 38.0 31.0 40.0 2.0 41.0 22 31.24373907545221 38.0 30.0 40.0 2.0 41.0 23 31.354195357398236 38.0 30.0 40.0 2.0 41.0 24 30.993007461797188 38.0 30.0 40.0 2.0 41.0 25 30.494733043777643 38.0 29.0 40.0 2.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 294337.0 3 9.0 4 2435.0 5 14099.0 6 16539.0 7 14272.0 8 11981.0 9 11080.0 10 10351.0 11 9992.0 12 9806.0 13 9435.0 14 9313.0 15 9789.0 16 10156.0 17 11473.0 18 13198.0 19 15964.0 20 20111.0 21 25289.0 22 31121.0 23 36661.0 24 41997.0 25 47986.0 26 53229.0 27 59982.0 28 66319.0 29 77694.0 30 92532.0 31 109632.0 32 137977.0 33 173793.0 34 223784.0 35 288331.0 36 381894.0 37 508341.0 38 773962.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.142849573508606 17.395589017332586 11.813158989159234 25.648402419999577 2 15.899714866942508 19.380159928454514 38.5992044980913 26.120920706511686 3 19.060924545960216 22.865963350138177 29.957106815232077 28.116005288669534 4 13.463619393204995 15.152796837951643 34.99630016504409 36.38728360379927 5 13.887520889013228 36.857365389501986 34.21963171328121 15.035482008203585 6 34.6846650504132 34.05432068249006 16.80375762377636 14.457256643320374 7 30.11824361605862 31.03028647730032 20.18760069743318 18.663869209207885 8 26.729202409949 34.58595174860344 20.15456271189059 18.530283129556967 9 27.04857766917569 15.542635990409002 18.86977194205563 38.539014398359676 10 15.74815835778097 28.01202509190969 33.030655321712864 23.20916122859648 11 36.20883547433011 22.42103694874851 21.77159206286143 19.598535514059947 12 23.41026284787269 24.35546125980472 29.28600397844686 22.948271913875733 13 28.93644657816114 21.054076401612665 25.118573111141586 24.890903909084614 14 22.747189893632726 20.30971801952858 25.72420991582166 31.218882171017036 15 24.595528898281472 27.771870628840066 22.90651959797054 24.72608087490792 16 24.390436397784523 26.95918986274281 24.70163070008922 23.948743039383448 17 23.500106113664984 27.04063201243669 25.555371876649836 23.903889997248495 18 23.57358873749331 26.758911276303373 26.304614162017188 23.362885824186133 19 24.50932539052961 26.122626919537723 26.453564811429693 22.91448287850297 20 24.95893663756937 25.8277995859588 25.891453660964658 23.321810115507173 21 25.370787645397048 25.425864269784952 25.562562464783795 23.640785620034205 22 24.06835446879479 24.98223959102089 26.9147376218571 24.034668318327224 23 24.61760381335297 25.30548514382158 25.820423544629236 24.25648749819621 24 24.123267103002906 25.551274106602378 26.117350573415564 24.20810821697915 25 23.901778668561075 25.2899334250048 26.67808818144853 24.130199724985594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1117.0 1 1117.0 2 1238.5 3 1360.0 4 1360.0 5 1360.0 6 1845.5 7 2331.0 8 2331.0 9 2331.0 10 3242.5 11 4154.0 12 4154.0 13 4154.0 14 6690.5 15 9227.0 16 9227.0 17 9227.0 18 16514.0 19 23801.0 20 23801.0 21 23801.0 22 38590.5 23 53380.0 24 53380.0 25 53380.0 26 79099.0 27 104818.0 28 104818.0 29 104818.0 30 137875.0 31 170932.0 32 170932.0 33 170932.0 34 209959.0 35 248986.0 36 248986.0 37 248986.0 38 294347.0 39 339708.0 40 339708.0 41 339708.0 42 380837.5 43 421967.0 44 421967.0 45 421967.0 46 455747.0 47 489527.0 48 489527.0 49 489527.0 50 497371.5 51 505216.0 52 505216.0 53 505216.0 54 476007.0 55 446798.0 56 446798.0 57 446798.0 58 394336.0 59 341874.0 60 341874.0 61 341874.0 62 284974.0 63 228074.0 64 228074.0 65 228074.0 66 179582.0 67 131090.0 68 131090.0 69 131090.0 70 97061.0 71 63032.0 72 63032.0 73 63032.0 74 43676.0 75 24320.0 76 24320.0 77 24320.0 78 16526.5 79 8733.0 80 8733.0 81 8733.0 82 5883.5 83 3034.0 84 3034.0 85 3034.0 86 2051.5 87 1069.0 88 1069.0 89 1069.0 90 666.0 91 263.0 92 263.0 93 263.0 94 151.0 95 39.0 96 39.0 97 39.0 98 26.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03384954580364946 2 0.0550089603361671 3 0.014124667849607598 4 0.010427977435843111 5 0.00968312190471146 6 5.517448378752968E-4 7 0.003972562832702137 8 0.001572472787944596 9 0.005931257007159441 10 0.0030345966083141327 11 0.010593500887205698 12 0.009214138792517458 13 0.008469283261385806 14 0.014759174413164192 15 0.007007159441016269 16 0.029876982970947322 17 0.0383738534742269 18 0.022180142482586932 19 0.01707650273224044 20 0.01619371099163996 21 0.0226767128366747 22 0.006317478393672149 23 0.015090221315889368 24 0.01219356091704406 25 0.4704452360143718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3624864.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.799288703436545 #Duplication Level Percentage of deduplicated Percentage of total 1 70.32597735964185 32.91205717805221 2 12.503739486466989 11.703322281994561 3 5.810769850054146 8.158196874057259 4 3.3662230893454153 6.301473847938007 5 2.018275231272638 4.722692261566167 6 1.3680578075446717 3.8414479378964113 7 0.9315233932597372 3.0516242550617108 8 0.697969097103927 2.6131565825154897 9 0.5271469620071834 2.2203092577702304 >10 2.3228810265336537 18.037791202321177 >50 0.09097803391443886 2.916121490901379 >100 0.03487880402705068 2.780766352836863 >500 0.0010329846210732313 0.33768969233375834 >1k 5.468742071689489E-4 0.4033507847548187 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.758724189376484E-5 2 0.0 0.0 0.0 0.0 2.758724189376484E-5 3 0.0 0.0 0.0 0.0 2.758724189376484E-5 4 0.0 0.0 0.0 1.1034896757505936E-4 2.758724189376484E-5 5 0.0 0.0 0.0 1.1034896757505936E-4 5.517448378752968E-5 6 0.0 0.0 0.0 1.1034896757505936E-4 5.517448378752968E-5 7 0.0 0.0 0.0 1.1034896757505936E-4 8.276172568129453E-5 8 0.0 0.0 0.0 1.1034896757505936E-4 8.276172568129453E-5 9 0.0 0.0 0.0 1.3793620946882422E-4 8.276172568129453E-5 10 0.0 0.0 0.0 1.6552345136258906E-4 8.276172568129453E-5 11 0.0 0.0 0.0 1.6552345136258906E-4 8.276172568129453E-5 12 0.0 0.0 0.0 1.6552345136258906E-4 2.7587241893764844E-4 13 0.0 0.0 0.0 1.6552345136258906E-4 2.7587241893764844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1590 0.0 13.325197 1 TAATACG 130 2.6357156E-9 11.687961 4 CGACCCG 75 2.08138E-4 11.396391 5 ACCGTGC 230 0.0 11.148182 8 GCGCAAC 60 0.005885435 11.081507 11 GTATCAA 3420 0.0 10.584363 1 GTCCTAG 505 0.0 9.78313 1 GAACCGT 215 1.0913936E-11 9.717049 6 GTATTAG 265 0.0 9.680188 1 AAGACCG 305 0.0 9.65268 5 GTCTAAG 325 0.0 9.647092 1 TCGCGGG 140 1.0618533E-6 9.498565 15 CTAATAC 245 1.8189894E-12 9.302791 3 TAAGACT 410 0.0 9.264847 4 TTTTACG 105 4.4988125E-4 9.046876 2 GTATAGG 360 0.0 8.973096 1 TAGACTG 340 0.0 8.938345 5 CTAAGAC 340 0.0 8.937976 3 GTCTAGA 500 0.0 8.930869 1 AGAACCG 235 6.730261E-11 8.8908 5 >>END_MODULE