FastQCFastQC Report
Mon 15 Aug 2016
SRR2921162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624864
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53410.14734345895459802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.013.5377511
CGTAAAC657.995857E-411.6978923
GTATCAA39500.010.93477251
CTAGGAC4850.010.582373
ACCGTGC2850.010.3315628
TGCACCG2107.2759576E-129.9517745
CGTGCGC2201.8189894E-129.93176110
TTAGGAC2300.09.9177783
CTATTAG1951.6007107E-109.7576761
TAGGACT4200.09.5044054
TAGGACA4750.09.4043594
GGTCGGT1651.0670192E-79.21206811
CCGTGCG1554.267058E-79.1932419
GTGCTAG4450.09.1930481
TAGGACG1356.813021E-69.15239054
ACACCGT2700.09.142296
ATTATAC2002.6375346E-99.029313
GTCTTAC1701.6499143E-78.9541031
AGGCCGT1606.8102236E-78.9005466
TAGGGTA1304.3749766E-58.7686955
Produced by FastQC (version 0.11.5)