##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3624864
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.58717789136365	31.0	30.0	33.0	26.0	34.0
2	29.863845098740256	31.0	30.0	34.0	26.0	34.0
3	30.004599896713366	31.0	30.0	34.0	26.0	34.0
4	33.688339479770825	35.0	35.0	37.0	30.0	37.0
5	33.212413210537	35.0	35.0	37.0	27.0	37.0
6	33.152583379679896	36.0	35.0	37.0	27.0	37.0
7	33.00295017964812	36.0	35.0	37.0	26.0	37.0
8	32.95111292451248	36.0	35.0	37.0	26.0	37.0
9	34.36844334021911	39.0	35.0	39.0	27.0	39.0
10	34.25303376899106	38.0	35.0	39.0	26.0	39.0
11	34.257498212346725	38.0	35.0	39.0	26.0	39.0
12	34.12425762732064	38.0	35.0	39.0	25.0	39.0
13	34.10765617689381	38.0	35.0	39.0	25.0	39.0
14	35.06690678602011	39.0	35.0	41.0	25.0	41.0
15	35.04711514694068	39.0	35.0	41.0	25.0	41.0
16	35.030827363454186	39.0	35.0	41.0	25.0	41.0
17	34.99971198919463	39.0	35.0	41.0	25.0	41.0
18	34.95144645426697	39.0	35.0	41.0	25.0	41.0
19	34.99292690705086	39.0	35.0	41.0	24.0	41.0
20	34.927865431641024	39.0	35.0	41.0	24.0	41.0
21	34.85744237576913	39.0	35.0	41.0	23.0	41.0
22	34.77501004175605	39.0	35.0	41.0	23.0	41.0
23	34.649829069449225	39.0	34.0	41.0	21.0	41.0
24	34.56821166256169	39.0	34.0	41.0	19.0	41.0
25	34.42709354061284	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114914.0
3	9.0
4	1867.0
5	16286.0
6	18154.0
7	13400.0
8	10760.0
9	9038.0
10	8312.0
11	7667.0
12	6903.0
13	6747.0
14	6562.0
15	6509.0
16	6604.0
17	6980.0
18	7703.0
19	9280.0
20	11512.0
21	14784.0
22	18435.0
23	22729.0
24	26786.0
25	31335.0
26	35418.0
27	39455.0
28	45490.0
29	55312.0
30	69747.0
31	89214.0
32	113736.0
33	146881.0
34	193530.0
35	256734.0
36	351762.0
37	531747.0
38	1312562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56228492906612	18.47766023811516	9.111344367830567	31.848710464988155
2	14.78320290085366	20.633849987199522	34.886357115742825	29.696589996203997
3	18.053268011374676	23.271913692000638	27.9684105480686	30.706407748556085
4	13.13832064473763	15.77218990688712	33.33751977403157	37.75196967434368
5	14.544121930043714	37.24387527071104	32.832689884206765	15.37931291503849
6	34.58066378002678	34.905728050712455	16.117443607611946	14.396164561648817
7	30.11054555486585	31.41794606524588	19.453948548175738	19.017559831712532
8	27.964133231250994	34.779067601961636	18.494423407205083	18.76237575958229
9	26.980684691345424	15.340821469607665	18.594257133753306	39.084236705293605
10	16.342400106818303	28.502030568462615	31.44088564289748	23.714683681821604
11	36.32539649037423	22.29265973768992	21.4473267817891	19.93461699014675
12	23.471181491799125	24.47662777410867	28.744601857507245	23.30758887658496
13	28.98971191655868	20.782226508885337	24.981798388215896	25.246263186340084
14	22.764101748772525	20.064125707585447	25.474980705978993	31.696791837663035
15	24.62659839066873	27.837893620344172	22.44700359867267	25.08850439031443
16	24.7736150249767	26.869058963553826	24.040150329224534	24.31717568224494
17	23.529970006819642	26.91234112332075	25.576855593540543	23.98083327631907
18	23.67047568018854	26.598791051830155	26.25065768182023	23.480075586161078
19	24.691750067249263	25.931316085334146	26.0165674596332	23.360366387783397
20	24.90671922299906	25.664497081087585	25.707340717814375	23.721442978098985
21	25.173872374945162	25.231374561628606	25.65836416763929	23.936388895786944
22	24.81005764757293	25.0919503065562	25.488139431204726	24.609852614666142
23	24.555970345879217	25.23276432169275	25.568250634761625	24.64301469766641
24	24.206687738520657	25.5448038088259	25.80639020158789	24.442118251065548
25	24.259744293640612	25.318886821223863	25.867429080532634	24.55393980460289
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	816.0
1	816.0
2	899.0
3	982.0
4	982.0
5	982.0
6	1391.5
7	1801.0
8	1801.0
9	1801.0
10	2489.0
11	3177.0
12	3177.0
13	3177.0
14	5042.5
15	6908.0
16	6908.0
17	6908.0
18	13325.5
19	19743.0
20	19743.0
21	19743.0
22	33346.5
23	46950.0
24	46950.0
25	46950.0
26	71278.0
27	95606.0
28	95606.0
29	95606.0
30	126507.0
31	157408.0
32	157408.0
33	157408.0
34	194127.0
35	230846.0
36	230846.0
37	230846.0
38	275239.5
39	319633.0
40	319633.0
41	319633.0
42	362466.0
43	405299.0
44	405299.0
45	405299.0
46	444164.0
47	483029.0
48	483029.0
49	483029.0
50	500741.5
51	518454.0
52	518454.0
53	518454.0
54	492631.5
55	466809.0
56	466809.0
57	466809.0
58	414957.0
59	363105.0
60	363105.0
61	363105.0
62	305570.0
63	248035.0
64	248035.0
65	248035.0
66	196295.0
67	144555.0
68	144555.0
69	144555.0
70	107584.0
71	70613.0
72	70613.0
73	70613.0
74	48743.5
75	26874.0
76	26874.0
77	26874.0
78	18161.5
79	9449.0
80	9449.0
81	9449.0
82	6424.5
83	3400.0
84	3400.0
85	3400.0
86	2221.0
87	1042.0
88	1042.0
89	1042.0
90	659.5
91	277.0
92	277.0
93	277.0
94	163.5
95	50.0
96	50.0
97	50.0
98	26.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10138311395958578
2	0.0
3	0.037960044845820426
4	0.05674695657547428
5	0.06783702781676774
6	0.009655534662817695
7	0.003144945575889192
8	0.00935207500198628
9	5.24157595981532E-4
10	4.6898311219400226E-4
11	0.010345215710161816
12	1.6552345136258904E-4
13	0.005103639750346496
14	0.05462273894965439
15	0.020690431420323632
16	0.007889951181616743
17	0.0011034896757505935
18	0.010786611580462053
19	0.007972712907298039
20	0.0015172983041570663
21	0.018042056198522206
22	0.002206979351501187
23	0.007586491520785331
24	0.01213838643325653
25	0.17054432938725425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3624864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.8735174006444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.59588705922272	20.783182043666418
2	15.008181238840496	10.467761391694577
3	7.73684966111187	8.094334838488635
4	4.67398670681506	6.519934270019825
5	2.96862858016539	5.176326022322401
6	2.116917521056455	4.4294616003774765
7	1.4999343547097013	3.6615590773155176
8	1.1069434125749067	3.0882408287967764
9	0.8626598424059141	2.7075584722481825
>10	4.16799642482261	24.607498394119723
>50	0.18041741484540363	4.2934971733043685
>100	0.07793357666558126	4.735106257943344
>500	0.002549013406286925	0.5874442526725204
>1k	0.0010355366892631373	0.6997272994436348
>5k	7.965666840485672E-5	0.14836807758658357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5341	0.14734345895459802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1034896757505936E-4
2	0.0	0.0	0.0	0.0	1.1034896757505936E-4
3	0.0	0.0	0.0	2.758724189376484E-5	1.1034896757505936E-4
4	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
5	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
6	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
7	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
8	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
9	0.0	0.0	0.0	1.931106932563539E-4	1.1034896757505936E-4
10	0.0	0.0	0.0	2.2069793515011872E-4	1.1034896757505936E-4
11	0.0	0.0	0.0	2.2069793515011872E-4	1.1034896757505936E-4
12	0.0	0.0	0.0	2.2069793515011872E-4	2.7587241893764844E-4
13	0.0	0.0	0.0	2.2069793515011872E-4	4.1380862840647263E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1785	0.0	13.537751	1
CGTAAAC	65	7.995857E-4	11.697892	3
GTATCAA	3950	0.0	10.9347725	1
CTAGGAC	485	0.0	10.58237	3
ACCGTGC	285	0.0	10.331562	8
TGCACCG	210	7.2759576E-12	9.951774	5
CGTGCGC	220	1.8189894E-12	9.931761	10
TTAGGAC	230	0.0	9.917778	3
CTATTAG	195	1.6007107E-10	9.757676	1
TAGGACT	420	0.0	9.504405	4
TAGGACA	475	0.0	9.404359	4
GGTCGGT	165	1.0670192E-7	9.212068	11
CCGTGCG	155	4.267058E-7	9.193241	9
GTGCTAG	445	0.0	9.193048	1
TAGGACG	135	6.813021E-6	9.1523905	4
ACACCGT	270	0.0	9.14229	6
ATTATAC	200	2.6375346E-9	9.02931	3
GTCTTAC	170	1.6499143E-7	8.954103	1
AGGCCGT	160	6.8102236E-7	8.900546	6
TAGGGTA	130	4.3749766E-5	8.768695	5
>>END_MODULE