FastQCFastQC Report
Mon 15 Aug 2016
SRR2921161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345927
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28860.1230217308552227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.011.8777471
CCGTCCA1251.8426363E-811.3960729
CGTAAAC803.784244E-410.6838193
CCGCACA803.7849892E-410.6835914
GTATTAG1701.382432E-1010.6201021
GTCCTAA1701.382432E-1010.6201021
GTGTAGG2400.010.2940461
CCGCGCA750.002656009510.1298439
TAAGGCG1054.1222036E-59.9489535
GTATCAA30800.09.7490081
GTGTTAC2450.09.6961191
GTACCGG900.0011116059.5021971
GTATAGA2002.6375346E-109.5021971
ACCGTGC1901.0586518E-99.4967278
CTCCTAA2900.09.1745351
ACCGTCC1151.11510504E-49.0838268
CTTAAAC2002.6739144E-99.0218923
CCCTTAT850.0074230228.9432441
GTTTAGG2451.6370905E-118.920431
CGTTCGC1502.708788E-68.86512515
Produced by FastQC (version 0.11.5)