##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921161_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345927
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.81448570224052	31.0	27.0	31.0	10.0	34.0
2	26.598841737189606	31.0	26.0	31.0	10.0	34.0
3	26.42611215097486	31.0	26.0	31.0	10.0	34.0
4	30.215854542788417	35.0	30.0	37.0	10.0	37.0
5	29.967478527677972	35.0	30.0	37.0	7.0	37.0
6	30.274676066220305	35.0	30.0	37.0	8.0	37.0
7	30.20691308808842	35.0	30.0	37.0	2.0	37.0
8	29.848211815627682	35.0	30.0	37.0	2.0	37.0
9	31.456375667273534	37.0	31.0	39.0	2.0	39.0
10	30.8084181647596	37.0	30.0	39.0	2.0	39.0
11	31.24300329890913	37.0	30.0	39.0	2.0	39.0
12	30.947256670817122	37.0	30.0	39.0	2.0	39.0
13	30.947906307400018	37.0	30.0	39.0	2.0	39.0
14	32.018576451867425	38.0	31.0	40.0	2.0	41.0
15	31.671193945932675	38.0	30.0	40.0	2.0	41.0
16	31.589260023862636	38.0	30.0	40.0	2.0	41.0
17	31.90787991271681	38.0	31.0	40.0	2.0	41.0
18	31.957355876802644	38.0	31.0	40.0	2.0	41.0
19	31.65885937627215	38.0	30.0	40.0	2.0	41.0
20	31.523301875974827	38.0	30.0	40.0	2.0	41.0
21	31.469694922305766	38.0	30.0	40.0	2.0	41.0
22	30.71414583659253	38.0	28.0	40.0	2.0	41.0
23	30.801487429063222	38.0	30.0	40.0	2.0	41.0
24	30.42882579040183	37.0	29.0	40.0	2.0	41.0
25	29.94252591832568	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	209148.0
3	16.0
4	1727.0
5	9715.0
6	11573.0
7	10030.0
8	8578.0
9	8003.0
10	7541.0
11	7146.0
12	6844.0
13	6652.0
14	6701.0
15	6752.0
16	7147.0
17	7773.0
18	9233.0
19	11158.0
20	13760.0
21	17535.0
22	21271.0
23	24730.0
24	28035.0
25	32114.0
26	35546.0
27	39904.0
28	44431.0
29	51828.0
30	60759.0
31	72323.0
32	90203.0
33	112999.0
34	144467.0
35	185668.0
36	244120.0
37	323816.0
38	466681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61218062291085	17.850321791645825	12.36039811508939	25.177099470353937
2	15.72141259884767	19.615182353987365	39.39623217692416	25.2671728702408
3	18.81925049060758	23.387624538436647	29.811929853881438	27.981195117074336
4	13.478576343385578	15.82237630228872	35.320747348954114	35.378300005371585
5	13.995354065959646	36.71332353102923	34.359363722749684	14.931958680261431
6	33.764851417619816	34.29471112202375	16.994216302116367	14.946221158240064
7	29.37122229720138	31.626366196798838	20.20089204833925	18.80151945766053
8	26.223181820119446	34.11579400568654	20.845095038556796	18.815929135637223
9	26.891530805210394	15.722158845787229	19.359298346130828	38.02701200287155
10	16.13222254099077	27.923300593008964	32.555599652660696	23.38887721333957
11	35.28283281727806	22.879643804629705	22.15818959030626	19.679333787785968
12	23.31981368636181	24.965191953692724	29.174812701590387	22.54018165835508
13	28.507714586927595	21.490985189817675	25.319183493900248	24.68211672935448
14	22.730105918726863	21.0728982099036	25.79954910785088	30.397446763518666
15	24.613208524439493	28.022958743237304	22.996811711960195	24.367021020363012
16	24.059924033231624	27.329360456687514	24.804412008002604	23.806303502078254
17	23.16023832510601	27.412504392264157	25.739264965254204	23.687992317375624
18	23.167230820853934	27.022139942926554	26.473917657507318	23.336711578712194
19	24.385205801135005	26.468477847967698	26.749948092998586	22.39636825789871
20	24.67790043094407	25.824455521694716	26.257914994704862	23.23972905265635
21	25.246345062588766	25.677829988969847	25.675442325345603	23.400382623095787
22	24.299586536407944	24.927433921066353	27.043903703353195	23.72907583917251
23	24.474430304193046	25.477734433288568	26.10692588092345	23.940909381594935
24	24.051915651561124	25.675516093400084	26.300502504422013	23.972065750616782
25	23.716208952756453	25.45217120554576	26.879041124227538	23.95257871747025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1338.0
1	1338.0
2	1427.0
3	1516.0
4	1516.0
5	1516.0
6	1877.5
7	2239.0
8	2239.0
9	2239.0
10	3027.0
11	3815.0
12	3815.0
13	3815.0
14	5860.0
15	7905.0
16	7905.0
17	7905.0
18	13782.5
19	19660.0
20	19660.0
21	19660.0
22	30118.5
23	40577.0
24	40577.0
25	40577.0
26	57805.0
27	75033.0
28	75033.0
29	75033.0
30	96945.0
31	118857.0
32	118857.0
33	118857.0
34	142862.5
35	166868.0
36	166868.0
37	166868.0
38	194730.0
39	222592.0
40	222592.0
41	222592.0
42	246652.0
43	270712.0
44	270712.0
45	270712.0
46	291107.5
47	311503.0
48	311503.0
49	311503.0
50	315722.0
51	319941.0
52	319941.0
53	319941.0
54	300001.5
55	280062.0
56	280062.0
57	280062.0
58	246163.0
59	212264.0
60	212264.0
61	212264.0
62	178434.5
63	144605.0
64	144605.0
65	144605.0
66	113239.0
67	81873.0
68	81873.0
69	81873.0
70	60196.0
71	38519.0
72	38519.0
73	38519.0
74	27309.0
75	16099.0
76	16099.0
77	16099.0
78	11355.5
79	6612.0
80	6612.0
81	6612.0
82	4486.0
83	2360.0
84	2360.0
85	2360.0
86	1547.0
87	734.0
88	734.0
89	734.0
90	474.5
91	215.0
92	215.0
93	215.0
94	118.0
95	21.0
96	21.0
97	21.0
98	14.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033078608157883854
2	0.05473316092103463
3	0.015601508486836974
4	0.010614141019733351
5	0.009377955921049546
6	9.804226644733616E-4
7	0.0038364365131566326
8	0.00157720167763106
9	0.00660719621710309
10	0.004006944802630261
11	0.010571513947364945
12	0.008738549835523442
13	0.007502364736839637
14	0.015132610690784495
15	0.006564569144734684
16	0.02945530700656926
17	0.03815122976972429
18	0.022507094210518913
19	0.016454049934205114
20	0.016155660427626263
21	0.022933364934202984
22	0.005712027697366542
23	0.01607040628288945
24	0.01176507197368034
25	0.4700060999340559
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2345927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.68807392902233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.11430469604846	33.913042197561786
2	12.044402325517666	11.487486970603499
3	5.7092981830942975	8.167963015147011
4	3.238531563981095	6.177573305784111
5	2.067100820170124	4.928802836550777
6	1.3446266408194456	3.8473592792598397
7	0.9364786112087532	3.126120287098982
8	0.6570336763876105	2.5066136426743757
9	0.5061690983271585	2.17244064434509
>10	2.2702245551484763	18.038170141357902
>50	0.08402058220588325	2.6941743789862813
>100	0.026142547249259213	2.0747858432487507
>500	0.0011111332278176003	0.42231279399987215
>1k	5.555666139087814E-4	0.4431546633817043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2886	0.1230217308552227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2788121710522108E-4
2	0.0	0.0	0.0	0.0	1.2788121710522108E-4
3	0.0	0.0	0.0	0.0	1.2788121710522108E-4
4	0.0	0.0	0.0	0.0	1.2788121710522108E-4
5	0.0	0.0	0.0	0.0	1.2788121710522108E-4
6	0.0	0.0	0.0	0.0	3.8364365131566327E-4
7	0.0	4.2627072368407033E-5	0.0	0.0	3.8364365131566327E-4
8	0.0	4.2627072368407033E-5	0.0	0.0	4.688977960524773E-4
9	0.0	4.2627072368407033E-5	0.0	0.0	5.541519407892914E-4
10	0.0	4.2627072368407033E-5	0.0	0.0	5.967790131576984E-4
11	0.0	4.2627072368407033E-5	0.0	0.0	5.967790131576984E-4
12	0.0	4.2627072368407033E-5	0.0	0.0	8.525414473681406E-4
13	0.0	4.2627072368407033E-5	0.0	0.0	0.0010230497368417686
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1600	0.0	11.877747	1
CCGTCCA	125	1.8426363E-8	11.396072	9
CGTAAAC	80	3.784244E-4	10.683819	3
CCGCACA	80	3.7849892E-4	10.683591	4
GTATTAG	170	1.382432E-10	10.620102	1
GTCCTAA	170	1.382432E-10	10.620102	1
GTGTAGG	240	0.0	10.294046	1
CCGCGCA	75	0.0026560095	10.129843	9
TAAGGCG	105	4.1222036E-5	9.948953	5
GTATCAA	3080	0.0	9.749008	1
GTGTTAC	245	0.0	9.696119	1
GTACCGG	90	0.001111605	9.502197	1
GTATAGA	200	2.6375346E-10	9.502197	1
ACCGTGC	190	1.0586518E-9	9.496727	8
CTCCTAA	290	0.0	9.174535	1
ACCGTCC	115	1.11510504E-4	9.083826	8
CTTAAAC	200	2.6739144E-9	9.021892	3
CCCTTAT	85	0.007423022	8.943244	1
GTTTAGG	245	1.6370905E-11	8.92043	1
CGTTCGC	150	2.708788E-6	8.865125	15
>>END_MODULE