FastQCFastQC Report
Mon 15 Aug 2016
SRR2921161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345927
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55720.23751804723676395No Hit
TATCAACGCAGAGTACTTTTTTTTT37130.1582743197038953No Hit
GGTATCAACGCAGAGTACTTTTTTT36750.15665449095389586No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24080.10264599026312413No Hit
GGTATCAACGCAGAGTACATGGGGG24010.10234760075654528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC456.7605195E-414.77882313
GGTATCA17950.014.0422351
CTAATCG500.001499145313.300940514
TAATCGG658.054405E-411.68740115
ATCGTTG752.0860795E-411.39327218
ACGCTCT855.3237854E-511.1767849
CCTATAC2600.010.9658233
GGGCCGT700.001500324610.849816
CCCTATA1951.8189894E-1210.7221382
CCCTTAT1252.2040331E-710.6530231
TCTAACC951.638161E-410.0039083
CTTATAC3150.09.9645621
CTAACAC2400.09.8997013
GCATAAG1252.5311947E-69.8920931
GTATCAA35200.09.6467361
CAGGACT5200.09.5037134
TTACCCT1701.6549166E-89.5037124
TACCCTG1701.6694685E-89.4986465
ATGCCGT1304.2767915E-69.4935856
ATAGCAC1856.6556822E-99.2468563
Produced by FastQC (version 0.11.5)