##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345927
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.483446415851816	31.0	30.0	33.0	26.0	34.0
2	29.75852275028166	31.0	30.0	34.0	26.0	34.0
3	29.89091987943359	31.0	30.0	34.0	26.0	34.0
4	33.56647585368172	35.0	35.0	37.0	28.0	37.0
5	33.087691134464116	35.0	35.0	37.0	27.0	37.0
6	33.01407162285953	36.0	35.0	37.0	26.0	37.0
7	32.86838891406254	36.0	33.0	37.0	26.0	37.0
8	32.810556764980326	36.0	33.0	37.0	26.0	37.0
9	34.19488287572461	38.0	35.0	39.0	25.0	39.0
10	34.08011886132859	38.0	34.0	39.0	25.0	39.0
11	34.07354832439373	38.0	34.0	39.0	25.0	39.0
12	33.95222826626745	38.0	34.0	39.0	25.0	39.0
13	33.928938965278974	38.0	34.0	39.0	25.0	39.0
14	34.86299658940794	39.0	34.0	41.0	24.0	41.0
15	34.84610049673327	39.0	34.0	41.0	24.0	41.0
16	34.829845088956304	39.0	34.0	41.0	24.0	41.0
17	34.8085477510596	39.0	34.0	41.0	24.0	41.0
18	34.76105053567311	39.0	34.0	41.0	23.0	41.0
19	34.79600601382737	39.0	34.0	41.0	23.0	41.0
20	34.73799312595831	39.0	34.0	41.0	22.0	41.0
21	34.666311014792875	39.0	34.0	41.0	21.0	41.0
22	34.580100744822836	39.0	34.0	41.0	19.0	41.0
23	34.446576555877485	39.0	34.0	41.0	18.0	41.0
24	34.359567880841986	39.0	34.0	41.0	18.0	41.0
25	34.2195601141894	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79108.0
3	3.0
4	1233.0
5	10990.0
6	12359.0
7	9367.0
8	7336.0
9	6222.0
10	5585.0
11	4990.0
12	4639.0
13	4501.0
14	4469.0
15	4382.0
16	4470.0
17	4680.0
18	5175.0
19	6319.0
20	7610.0
21	9879.0
22	12518.0
23	15185.0
24	17621.0
25	20511.0
26	23680.0
27	26042.0
28	30398.0
29	36848.0
30	46395.0
31	58857.0
32	74820.0
33	96470.0
34	126356.0
35	167812.0
36	226236.0
37	342318.0
38	830543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20361228329927	18.93756373550884	9.415318709545286	31.443505271646604
2	14.796155208580657	20.941657604861533	35.16388191107396	29.09830527548385
3	17.770928014513007	23.664235749367464	28.211021987427348	30.353814248692178
4	13.112890719299697	16.368689397616464	33.207781672329354	37.310638210754476
5	14.572969569672217	37.54219798951161	32.54050452457998	15.344327916236193
6	34.05063544754153	35.155448377054235	16.206267555109562	14.587648620294678
7	29.768452623775904	32.03669530796752	19.305831034654737	18.889021033601843
8	27.501480279353974	34.84724668040161	18.656550038983887	18.99472300126053
9	27.080169161273986	15.492383181573851	18.9569411153885	38.47050654176366
10	16.872008378777345	28.556174169102448	30.90509636489115	23.666721087229057
11	35.655269489360755	22.68799630324949	21.711119750503443	19.945614456886307
12	23.549283502854095	25.012926659695715	28.599909545352435	22.83788029209775
13	28.667164273903374	21.08322928624622	25.23512368211167	25.014482757738737
14	22.882564645447033	20.78387482843248	25.597319409457985	30.736241116662498
15	24.736743817988483	27.93726022561283	22.535743917756797	24.79025203864189
16	24.49079027575888	27.158349283214474	24.144951383057176	24.20590905796947
17	23.266666013051555	27.31133577472786	25.62215277798499	23.799845434235593
18	23.369884701839137	26.70814734735793	26.450157747936657	23.471810202866276
19	24.558376867938282	26.242084375073265	26.416776968542116	22.782761788446333
20	24.698210984399772	25.62006405143894	26.17029430157034	23.51143066259095
21	25.122214183794274	25.390324413804628	25.826488472198005	23.66097293020309
22	25.05149356927164	25.01632618959523	25.64222029358155	24.289959947551583
23	24.47320588195174	25.30696265124337	25.92804942094422	24.29178204586067
24	24.0180527053054	25.745822956622867	26.029649611773902	24.20647472629783
25	24.16245518967126	25.363702939813777	26.079515247788578	24.39432662272639
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	902.0
1	902.0
2	944.5
3	987.0
4	987.0
5	987.0
6	1379.5
7	1772.0
8	1772.0
9	1772.0
10	2326.5
11	2881.0
12	2881.0
13	2881.0
14	4246.5
15	5612.0
16	5612.0
17	5612.0
18	10747.0
19	15882.0
20	15882.0
21	15882.0
22	25924.5
23	35967.0
24	35967.0
25	35967.0
26	52537.5
27	69108.0
28	69108.0
29	69108.0
30	89124.5
31	109141.0
32	109141.0
33	109141.0
34	132050.0
35	154959.0
36	154959.0
37	154959.0
38	182521.5
39	210084.0
40	210084.0
41	210084.0
42	234480.5
43	258877.0
44	258877.0
45	258877.0
46	284075.0
47	309273.0
48	309273.0
49	309273.0
50	318284.5
51	327296.0
52	327296.0
53	327296.0
54	310550.0
55	293804.0
56	293804.0
57	293804.0
58	260622.5
59	227441.0
60	227441.0
61	227441.0
62	193120.0
63	158799.0
64	158799.0
65	158799.0
66	124852.5
67	90906.0
68	90906.0
69	90906.0
70	66967.0
71	43028.0
72	43028.0
73	43028.0
74	30399.5
75	17771.0
76	17771.0
77	17771.0
78	12582.5
79	7394.0
80	7394.0
81	7394.0
82	5133.0
83	2872.0
84	2872.0
85	2872.0
86	1863.5
87	855.0
88	855.0
89	855.0
90	564.0
91	273.0
92	273.0
93	273.0
94	157.5
95	42.0
96	42.0
97	42.0
98	21.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09620930233549467
2	0.0
3	0.042456564078933404
4	0.05716290404603382
5	0.0690984843091878
6	0.004262707236840703
7	5.967790131576984E-4
8	0.0036659282236830046
9	0.0
10	0.0
11	0.003538047006577784
12	0.0
13	0.001449320460525839
14	0.05835646207234922
15	0.021015146677624667
16	0.00157720167763106
17	0.0
18	0.0040921989473670746
19	0.0039216906578934466
20	0.0
21	0.010656768092101757
22	1.2788121710522108E-4
23	0.0016624558223678743
24	0.005498892335524507
25	0.1752825215788897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2345927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.58074033943209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.135744601992634	20.795385691999122
2	14.333626392414644	9.913348247970431
3	7.489786778425196	7.7700711534729985
4	4.589404787902749	6.348200611320454
5	3.0760072193130665	5.3185303466641844
6	2.144564070160586	4.449636795089932
7	1.5452560154700394	3.740526792024042
8	1.1510136983776302	3.1842324664580994
9	0.8831863273033933	2.748711335023379
>10	4.392597643494138	25.752667132837608
>50	0.1920745461175758	4.516986363408329
>100	0.06274896827250528	3.6871799801459493
>500	0.0023684395113249836	0.5494788356037815
>1k	0.0014958565334683117	0.9848547075420855
>5k	1.2465471112235932E-4	0.24018954043964197
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5572	0.23751804723676395	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3713	0.1582743197038953	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3675	0.15665449095389586	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2408	0.10264599026312413	No Hit
GGTATCAACGCAGAGTACATGGGGG	2401	0.10234760075654528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7050828947362813E-4
2	0.0	0.0	0.0	0.0	1.7050828947362813E-4
3	0.0	0.0	0.0	0.0	1.7050828947362813E-4
4	0.0	0.0	0.0	0.0	2.5576243421044216E-4
5	0.0	0.0	0.0	0.0	2.5576243421044216E-4
6	0.0	0.0	0.0	0.0	3.8364365131566327E-4
7	0.0	0.0	0.0	0.0	3.8364365131566327E-4
8	0.0	0.0	0.0	0.0	5.115248684208843E-4
9	0.0	0.0	0.0	0.0	5.967790131576984E-4
10	0.0	0.0	0.0	0.0	5.967790131576984E-4
11	0.0	0.0	0.0	0.0	5.967790131576984E-4
12	0.0	0.0	0.0	0.0	8.951685197365476E-4
13	0.0	0.0	0.0	0.0	0.0012361850986838039
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGC	45	6.7605195E-4	14.778823	13
GGTATCA	1795	0.0	14.042235	1
CTAATCG	50	0.0014991453	13.3009405	14
TAATCGG	65	8.054405E-4	11.687401	15
ATCGTTG	75	2.0860795E-4	11.393272	18
ACGCTCT	85	5.3237854E-5	11.176784	9
CCTATAC	260	0.0	10.965823	3
GGGCCGT	70	0.0015003246	10.84981	6
CCCTATA	195	1.8189894E-12	10.722138	2
CCCTTAT	125	2.2040331E-7	10.653023	1
TCTAACC	95	1.638161E-4	10.003908	3
CTTATAC	315	0.0	9.964562	1
CTAACAC	240	0.0	9.899701	3
GCATAAG	125	2.5311947E-6	9.892093	1
GTATCAA	3520	0.0	9.646736	1
CAGGACT	520	0.0	9.503713	4
TTACCCT	170	1.6549166E-8	9.503712	4
TACCCTG	170	1.6694685E-8	9.498646	5
ATGCCGT	130	4.2767915E-6	9.493585	6
ATAGCAC	185	6.6556822E-9	9.246856	3
>>END_MODULE