FastQCFastQC Report
Mon 15 Aug 2016
SRR2921160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595123
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32050.1235008899385501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG350.002168623516.2880762
GGTATCA14100.012.19821551
CCGTCTA907.493878E-611.6072019
GTATCAA31300.010.7168521
TTTTACG803.7674524E-410.6890512
ACCGTCC1553.6870915E-910.4158478
TAGTACT2107.2759576E-129.9492234
TAAGGCG1054.122312E-59.949035
GTGCTAG2300.09.9156281
TAGCACT2350.09.6990484
GTATTAC1606.613118E-89.5024781
GCGTATG1304.249636E-69.49844815
CGTGTCA1401.0629283E-69.49753310
TAATACT3300.09.49698454
TAAGATA1804.2073225E-99.49698454
TACACCG1002.7657722E-49.4968015
ACACCGT2303.6379788E-129.4966196
CCAGTAC2750.09.3246713
TTACGGA1651.0079384E-79.24589819
GTCCTAC1451.7011098E-69.1748071
Produced by FastQC (version 0.11.5)