##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921160_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2595123
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.05379398201935	31.0	28.0	31.0	10.0	34.0
2	26.907280310027694	31.0	27.0	31.0	10.0	34.0
3	26.664198575558846	31.0	26.0	31.0	10.0	34.0
4	30.578161035141687	35.0	31.0	37.0	10.0	37.0
5	30.234769219031236	35.0	30.0	37.0	10.0	37.0
6	30.611329790534015	35.0	31.0	37.0	10.0	37.0
7	30.56617008134104	35.0	31.0	37.0	8.0	37.0
8	30.15432062372381	35.0	30.0	37.0	8.0	37.0
9	31.85671392068892	37.0	32.0	39.0	7.0	39.0
10	31.228159127717646	37.0	30.0	39.0	2.0	39.0
11	31.661984036980137	37.0	32.0	39.0	2.0	39.0
12	31.37602572209487	37.0	31.0	39.0	2.0	39.0
13	31.379063343047708	37.0	31.0	39.0	2.0	39.0
14	32.490383692796065	38.0	32.0	40.0	2.0	41.0
15	32.142478795802745	38.0	31.0	40.0	2.0	41.0
16	32.05961991011601	38.0	31.0	40.0	2.0	41.0
17	32.37710967842372	38.0	32.0	40.0	2.0	41.0
18	32.42950257078373	38.0	32.0	40.0	2.0	41.0
19	32.147998765376435	38.0	31.0	40.0	2.0	41.0
20	32.013762353460706	38.0	31.0	40.0	2.0	41.0
21	31.973581213684284	38.0	31.0	40.0	2.0	41.0
22	31.2037182823319	38.0	30.0	40.0	2.0	41.0
23	31.31242025907828	38.0	30.0	40.0	2.0	41.0
24	30.944764853149543	38.0	30.0	40.0	2.0	41.0
25	30.44533226363452	38.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	212824.0
3	10.0
4	1787.0
5	10194.0
6	11835.0
7	10382.0
8	8943.0
9	8079.0
10	7745.0
11	7372.0
12	7067.0
13	6888.0
14	6960.0
15	6981.0
16	7476.0
17	8176.0
18	9475.0
19	11438.0
20	14503.0
21	18082.0
22	22261.0
23	26239.0
24	30055.0
25	33833.0
26	38359.0
27	42699.0
28	47752.0
29	55784.0
30	65780.0
31	78742.0
32	98445.0
33	124673.0
34	160399.0
35	209269.0
36	276911.0
37	370409.0
38	537296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.461092443680016	17.857298421618054	12.504167888926194	25.177441245775732
2	15.778023068556802	19.485285187799736	39.15632480731544	25.580366936328026
3	19.08779578267609	23.372795431964686	29.393084279419096	28.14632450594012
4	13.446940720498313	15.522673240731713	35.22864389097914	35.801742147790826
5	13.935660696420783	36.52586567023833	34.30791084556132	15.230562787779572
6	34.06523494939717	34.067277250461636	17.230277537154464	14.637210262986722
7	29.47269568085752	31.738065354953644	20.327903850014778	18.46133511417406
8	26.653598362749864	34.15848356295348	20.211492272116153	18.976425802180508
9	27.08168831063732	15.687437402430646	18.823568401536985	38.40730588539505
10	15.858883317585368	28.198057528118987	32.71177675600106	23.231282398294596
11	35.83657403107601	22.609440267174026	21.899768889532897	19.65421681221706
12	23.529835670854602	24.55838613534013	29.115801140230467	22.795977053574802
13	28.81462658567847	21.30325640687706	25.220910184643536	24.66120682280094
14	22.781662566056816	20.48239204296699	25.824053005777852	30.911892385198342
15	24.67275925786767	27.95747903142829	22.86043495937309	24.50932675133095
16	24.134245313382323	27.105465520331645	24.720017114080377	24.040272052205655
17	23.471832028346835	27.14983547610163	25.673399245687996	23.70493324986354
18	23.34197069924892	26.888378329731538	26.347242151887368	23.42240881913217
19	24.699388139243414	26.07595922161569	26.484020552601677	22.740632086539218
20	24.81498025030032	25.630982575018606	26.03599690602812	23.518040268652953
21	25.426607085181125	25.319574707212293	25.741807224550616	23.51201098305597
22	24.411355886286994	24.748467141864573	26.897455056649566	23.94272191519887
23	24.656041752498055	25.211207841774	26.001428285683343	24.131322120044597
24	24.242024848535998	25.382403006581132	26.232821201761496	24.14275094312137
25	23.82598596661021	25.372523590612143	26.67218969271715	24.12930075006049
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1149.0
1	1149.0
2	1220.0
3	1291.0
4	1291.0
5	1291.0
6	1684.0
7	2077.0
8	2077.0
9	2077.0
10	2821.0
11	3565.0
12	3565.0
13	3565.0
14	5465.5
15	7366.0
16	7366.0
17	7366.0
18	13030.5
19	18695.0
20	18695.0
21	18695.0
22	29809.5
23	40924.0
24	40924.0
25	40924.0
26	60910.0
27	80896.0
28	80896.0
29	80896.0
30	106481.5
31	132067.0
32	132067.0
33	132067.0
34	157315.0
35	182563.0
36	182563.0
37	182563.0
38	213377.5
39	244192.0
40	244192.0
41	244192.0
42	270433.5
43	296675.0
44	296675.0
45	296675.0
46	318821.5
47	340968.0
48	340968.0
49	340968.0
50	345393.0
51	349818.0
52	349818.0
53	349818.0
54	331244.5
55	312671.0
56	312671.0
57	312671.0
58	277999.0
59	243327.0
60	243327.0
61	243327.0
62	204442.5
63	165558.0
64	165558.0
65	165558.0
66	130904.0
67	96250.0
68	96250.0
69	96250.0
70	71449.5
71	46649.0
72	46649.0
73	46649.0
74	32645.5
75	18642.0
76	18642.0
77	18642.0
78	12659.0
79	6676.0
80	6676.0
81	6676.0
82	4462.0
83	2248.0
84	2248.0
85	2248.0
86	1458.5
87	669.0
88	669.0
89	669.0
90	411.5
91	154.0
92	154.0
93	154.0
94	91.0
95	28.0
96	28.0
97	28.0
98	16.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03406389600801195
2	0.053022534962697335
3	0.015297926148394507
4	0.01078946932380469
5	0.009826123848464987
6	4.2387200914947E-4
7	0.003622178987277289
8	0.0015413527605435272
9	0.006704884508364343
10	0.0030441717020734662
11	0.010018792943532927
12	0.008053568173839929
13	0.0068204859654051085
14	0.014912587958258627
15	0.006473681594282814
16	0.02959397300243572
17	0.03741633826219412
18	0.02242668266590832
19	0.015991534890639095
20	0.015413527605435273
21	0.02238814884689473
22	0.005163531747820816
23	0.015567662881489626
24	0.010866536961831865
25	0.462328760525031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2595123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.49489802326407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75426184730034	31.734630294309884
2	12.354309459656918	11.241160980298762
3	5.916588007104113	8.075237040866066
4	3.3926193180740327	6.1738747963013525
5	2.1399907279026262	4.867932996833032
6	1.45148055870245	3.9620976000550994
7	1.0337454074927765	3.2921099327130996
8	0.7285735909215508	2.6517104977135415
9	0.5631672007551064	2.3059110931560585
>10	2.5345051340980205	19.173313405690227
>50	0.09207910725259019	2.839555700154429
>100	0.03700526996227538	2.8540389263231463
>500	9.693725552658702E-4	0.2918717821744457
>1k	7.049982220090478E-4	0.5365549534107001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3205	0.1235008899385501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.706763802717637E-5
2	0.0	0.0	0.0	0.0	7.706763802717637E-5
3	0.0	0.0	0.0	0.0	7.706763802717637E-5
4	0.0	0.0	0.0	0.0	7.706763802717637E-5
5	0.0	0.0	0.0	0.0	1.1560145704076455E-4
6	0.0	0.0	0.0	0.0	1.1560145704076455E-4
7	0.0	0.0	0.0	0.0	1.5413527605435273E-4
8	0.0	0.0	0.0	0.0	1.5413527605435273E-4
9	0.0	0.0	0.0	0.0	1.5413527605435273E-4
10	0.0	0.0	0.0	0.0	1.5413527605435273E-4
11	0.0	0.0	0.0	0.0	1.5413527605435273E-4
12	0.0	0.0	0.0	0.0	5.009396471766463E-4
13	0.0	0.0	0.0	0.0	6.165411042174109E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	0.0021686235	16.288076	2
GGTATCA	1410	0.0	12.1982155	1
CCGTCTA	90	7.493878E-6	11.607201	9
GTATCAA	3130	0.0	10.716852	1
TTTTACG	80	3.7674524E-4	10.689051	2
ACCGTCC	155	3.6870915E-9	10.415847	8
TAGTACT	210	7.2759576E-12	9.949223	4
TAAGGCG	105	4.122312E-5	9.94903	5
GTGCTAG	230	0.0	9.915628	1
TAGCACT	235	0.0	9.699048	4
GTATTAC	160	6.613118E-8	9.502478	1
GCGTATG	130	4.249636E-6	9.498448	15
CGTGTCA	140	1.0629283E-6	9.497533	10
TAATACT	330	0.0	9.4969845	4
TAAGATA	180	4.2073225E-9	9.4969845	4
TACACCG	100	2.7657722E-4	9.496801	5
ACACCGT	230	3.6379788E-12	9.496619	6
CCAGTAC	275	0.0	9.324671	3
TTACGGA	165	1.0079384E-7	9.245898	19
GTCCTAC	145	1.7011098E-6	9.174807	1
>>END_MODULE