FastQCFastQC Report
Mon 15 Aug 2016
SRR2921160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595123
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59730.2301625009681622No Hit
GGTATCAACGCAGAGTACTTTTTTT38650.1489332104875183No Hit
TATCAACGCAGAGTACTTTTTTTTT37690.14523396386221385No Hit
GGTATCAACGCAGAGTACATGGGGG28540.10997551946478068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.005282636714.2490755
GGTATCA17100.014.1884541
ATTACGA707.271905E-613.5705475
ACCGACC1001.4472607E-712.3477698
ATTCGCC951.0407421E-611.99991611
CGATTCG802.8694321E-511.8744589
TCGCGCA1052.7438182E-711.75479518
CCGGGTA855.2730844E-511.18739319
GATTCGC855.326196E-511.17639110
TACGATT855.365027E-511.1684247
CCATGCG909.524199E-510.5550739
GTATCAA36150.010.4489621
ACAACCG1106.053786E-610.3629645
ATAACCC1803.9108272E-1010.0329293
CGCCGAA951.644903E-49.99992912
GTTCTAG1252.5214613E-69.8951941
GCCGGGT1356.500468E-79.84589718
TAGGACA2850.09.6716344
GTCGTAG800.0044602669.5146091
CTAACAC2600.09.5048813
Produced by FastQC (version 0.11.5)