##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2595123
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.624673281382037	31.0	30.0	33.0	26.0	34.0
2	29.882066861570724	31.0	30.0	34.0	26.0	34.0
3	29.99176994693508	31.0	30.0	34.0	26.0	34.0
4	33.68057082458134	35.0	35.0	37.0	30.0	37.0
5	33.20296918489027	35.0	35.0	37.0	27.0	37.0
6	33.14547364421648	36.0	35.0	37.0	27.0	37.0
7	33.01290767335498	36.0	35.0	37.0	26.0	37.0
8	32.94257227884767	36.0	35.0	37.0	26.0	37.0
9	34.35122381482496	39.0	35.0	39.0	26.0	39.0
10	34.21655659481265	38.0	35.0	39.0	26.0	39.0
11	34.22423561426568	38.0	35.0	39.0	26.0	39.0
12	34.10056440484709	38.0	35.0	39.0	25.0	39.0
13	34.07878200763509	38.0	35.0	39.0	25.0	39.0
14	35.03637746650159	39.0	35.0	41.0	25.0	41.0
15	35.02331411651779	39.0	35.0	41.0	25.0	41.0
16	35.010189883099954	39.0	35.0	41.0	25.0	41.0
17	34.988788200019805	39.0	35.0	41.0	25.0	41.0
18	34.94376836858985	39.0	35.0	41.0	25.0	41.0
19	34.986798698944135	39.0	35.0	41.0	24.0	41.0
20	34.91760621750876	39.0	35.0	41.0	24.0	41.0
21	34.84173312787101	39.0	35.0	41.0	23.0	41.0
22	34.75955937348634	39.0	35.0	41.0	22.0	41.0
23	34.631009397242444	39.0	34.0	41.0	19.0	41.0
24	34.54956239068437	39.0	34.0	41.0	19.0	41.0
25	34.40704313437167	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83649.0
3	5.0
4	1312.0
5	11630.0
6	13309.0
7	9934.0
8	7842.0
9	6735.0
10	5964.0
11	5437.0
12	4992.0
13	4807.0
14	4813.0
15	4586.0
16	4717.0
17	5084.0
18	5458.0
19	6476.0
20	8208.0
21	10556.0
22	13190.0
23	16050.0
24	19104.0
25	22040.0
26	24860.0
27	28205.0
28	32470.0
29	38982.0
30	49813.0
31	62883.0
32	80999.0
33	105100.0
34	138127.0
35	182525.0
36	250739.0
37	380391.0
38	944131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67218929798602	18.771764840207304	9.378688547354583	31.177357314452088
2	14.899447925974993	20.750307403541182	35.37354491482677	28.976699755657055
3	18.1586883537075	23.738605186555557	27.7153550833387	30.387351376398247
4	13.27323184733611	16.211786604897053	33.0736423767525	37.44133917101434
5	14.638757830561024	37.45365291212997	32.65052319427118	15.257066063037824
6	34.423942532988036	34.87059131828833	16.21851492169195	14.486951227031694
7	30.107637512094914	31.708732499222574	19.40958707163002	18.77404291705249
8	27.949295907085403	34.93022271772428	18.372892355347933	18.74758901984239
9	27.262715485932652	15.590744639078762	18.51688725351361	38.629652621474975
10	16.817931173204506	28.684883144267147	30.902774165232245	23.594411517296095
11	36.107827930054434	22.52771328098656	21.57895852401368	19.785500264945323
12	23.86068020667999	24.721255986710457	28.47105898256075	22.947004824048804
13	28.976454294696264	20.97472175744633	25.08614517398204	24.962678773875375
14	22.931797675691932	20.31968875556207	25.565537930023986	31.182975638722016
15	24.848847378151927	27.95434144401196	22.45827139290894	24.73853978492717
16	24.686058157516783	27.013969626822792	23.976497762801895	24.32347445285853
17	23.546359844986153	27.122067046533054	25.613429498332064	23.71814361014873
18	23.57519233222306	26.69721167893901	26.329677257993595	23.397918730844335
19	24.930595269842197	25.85605589990304	26.085658000003082	23.127690830251677
20	24.90224933461728	25.464265084930464	26.012138923665663	23.621346656786596
21	25.262710809405597	25.162810362975474	25.832590439388525	23.7418883882304
22	25.1831996914207	24.92028303904368	25.54653563092868	24.349981638606938
23	24.678080742725484	25.0709927579487	25.87122921421836	24.379697285107454
24	24.320953778610892	25.434211905584057	25.932513133879244	24.312321181925807
25	24.368078237509707	25.294087445873743	25.86695624077629	24.470878075840265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	788.0
1	788.0
2	858.5
3	929.0
4	929.0
5	929.0
6	1230.0
7	1531.0
8	1531.0
9	1531.0
10	2206.0
11	2881.0
12	2881.0
13	2881.0
14	4123.0
15	5365.0
16	5365.0
17	5365.0
18	10645.0
19	15925.0
20	15925.0
21	15925.0
22	26021.5
23	36118.0
24	36118.0
25	36118.0
26	55235.5
27	74353.0
28	74353.0
29	74353.0
30	97717.5
31	121082.0
32	121082.0
33	121082.0
34	145378.5
35	169675.0
36	169675.0
37	169675.0
38	199873.5
39	230072.0
40	230072.0
41	230072.0
42	257214.5
43	284357.0
44	284357.0
45	284357.0
46	310247.0
47	336137.0
48	336137.0
49	336137.0
50	347058.5
51	357980.0
52	357980.0
53	357980.0
54	342951.0
55	327922.0
56	327922.0
57	327922.0
58	293481.5
59	259041.0
60	259041.0
61	259041.0
62	220085.5
63	181130.0
64	181130.0
65	181130.0
66	143903.5
67	106677.0
68	106677.0
69	106677.0
70	79135.5
71	51594.0
72	51594.0
73	51594.0
74	36076.0
75	20558.0
76	20558.0
77	20558.0
78	14035.5
79	7513.0
80	7513.0
81	7513.0
82	5020.0
83	2527.0
84	2527.0
85	2527.0
86	1636.5
87	746.0
88	746.0
89	746.0
90	468.5
91	191.0
92	191.0
93	191.0
94	110.5
95	30.0
96	30.0
97	30.0
98	15.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10169074837685921
2	0.0
3	0.04123118634453935
4	0.053908812800009864
5	0.07390786486806213
6	0.009363718020301929
7	9.248116563261163E-4
8	0.008631575459043753
9	0.0
10	0.0
11	0.00955638711536987
12	0.0
13	0.0032753746161549957
14	0.06408174101959715
15	0.02157893864760938
16	0.005240599385847993
17	0.0
18	0.010172928219587278
19	0.007668229983704048
20	0.0
21	0.020808262267337615
22	1.5413527605435273E-4
23	0.005394734661902345
24	0.011829882437171571
25	0.17925932605121223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2595123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.52693580759371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.69868353956019	20.01513930828686
2	14.231583211447528	9.542827535412593
3	7.522600102258646	7.566291922038704
4	4.6473174583915675	6.232412564200145
5	3.1198369566611452	5.229928668806836
6	2.228878504368009	4.483647992332303
7	1.5735165127035544	3.6928630979520545
8	1.1952462928510916	3.205835658774632
9	0.9121098698447292	2.75222241501812
>10	4.582155099143186	26.26837337061013
>50	0.20253828743834928	4.600126900063222
>100	0.08146659412661011	4.727329439525545
>500	0.002905408003828267	0.6526042014116473
>1k	0.0010459468813776914	0.7976663210516692
>5k	1.1621632015307683E-4	0.23273060451551486
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5973	0.2301625009681622	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3865	0.1489332104875183	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3769	0.14523396386221385	No Hit
GGTATCAACGCAGAGTACATGGGGG	2854	0.10997551946478068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1560145704076455E-4
2	0.0	0.0	0.0	0.0	1.1560145704076455E-4
3	0.0	0.0	0.0	0.0	1.1560145704076455E-4
4	0.0	0.0	0.0	0.0	1.1560145704076455E-4
5	0.0	0.0	0.0	0.0	1.1560145704076455E-4
6	0.0	0.0	0.0	0.0	1.1560145704076455E-4
7	0.0	0.0	0.0	0.0	1.1560145704076455E-4
8	0.0	0.0	0.0	0.0	1.1560145704076455E-4
9	0.0	0.0	0.0	3.853381901358818E-5	1.1560145704076455E-4
10	0.0	0.0	0.0	7.706763802717637E-5	1.1560145704076455E-4
11	0.0	0.0	0.0	7.706763802717637E-5	1.1560145704076455E-4
12	0.0	0.0	0.0	7.706763802717637E-5	8.862778373125281E-4
13	0.0	0.0	0.0	7.706763802717637E-5	9.248116563261164E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0052826367	14.249075	5
GGTATCA	1710	0.0	14.188454	1
ATTACGA	70	7.271905E-6	13.570547	5
ACCGACC	100	1.4472607E-7	12.347769	8
ATTCGCC	95	1.0407421E-6	11.999916	11
CGATTCG	80	2.8694321E-5	11.874458	9
TCGCGCA	105	2.7438182E-7	11.754795	18
CCGGGTA	85	5.2730844E-5	11.187393	19
GATTCGC	85	5.326196E-5	11.176391	10
TACGATT	85	5.365027E-5	11.168424	7
CCATGCG	90	9.524199E-5	10.555073	9
GTATCAA	3615	0.0	10.448962	1
ACAACCG	110	6.053786E-6	10.362964	5
ATAACCC	180	3.9108272E-10	10.032929	3
CGCCGAA	95	1.644903E-4	9.999929	12
GTTCTAG	125	2.5214613E-6	9.895194	1
GCCGGGT	135	6.500468E-7	9.845897	18
TAGGACA	285	0.0	9.671634	4
GTCGTAG	80	0.004460266	9.514609	1
CTAACAC	260	0.0	9.504881	3
>>END_MODULE