FastQCFastQC Report
Mon 15 Aug 2016
SRR2921159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181142
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22160.18761503697269252No Hit
GGTATCAACGCAGAGTACTTTTTTT11970.1013425989423795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.014.0831191
TACGAGA551.9658326E-413.8129474
AGCACCG1001.4484067E-712.3453225
GTCTTAG1209.944415E-911.8795551
CGTGCGC658.043594E-411.68837310
CTTAGAC752.0794998E-411.3961643
TCAACTC1950.011.2008835
CTATACT1201.2805322E-711.0791354
GTCCTAG1658.0035534E-1110.943591
TAGACAG1502.1427695E-910.7625885
GTATCAA21850.010.65626051
GTATAAT1252.2255699E-710.6440811
GTTATAT909.4728064E-510.5596041
TCCTATA750.002642068310.1355032
AAGGCGT750.0026560310.12906556
TACACAA1606.199116E-910.0899275
GTCATAA856.567243E-410.0626821
GTACACG856.567243E-410.0626821
TCTATAC1054.1171377E-59.9490313
GTGGTAT3350.09.929181
Produced by FastQC (version 0.11.5)