##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921159_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181142
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.769968386527616	31.0	27.0	31.0	7.0	34.0
2	26.551082765662386	31.0	26.0	31.0	7.0	34.0
3	26.38931390129214	31.0	26.0	31.0	7.0	34.0
4	30.25495410374028	35.0	30.0	37.0	10.0	37.0
5	30.05041646135689	35.0	30.0	37.0	7.0	37.0
6	30.280034915361576	35.0	31.0	37.0	2.0	37.0
7	30.246475021631607	35.0	31.0	37.0	2.0	37.0
8	29.796866930479148	35.0	30.0	37.0	2.0	37.0
9	31.43309695193296	37.0	31.0	39.0	2.0	39.0
10	30.831271769186092	37.0	30.0	39.0	2.0	39.0
11	31.246870401695986	37.0	31.0	39.0	2.0	39.0
12	30.960955583663946	37.0	30.0	39.0	2.0	39.0
13	30.95401399662361	37.0	30.0	39.0	2.0	39.0
14	32.03461480499381	38.0	31.0	40.0	2.0	41.0
15	31.690293800406725	38.0	30.0	40.0	2.0	41.0
16	31.610196741797346	38.0	30.0	40.0	2.0	41.0
17	31.9219856714942	38.0	31.0	40.0	2.0	41.0
18	31.971765460884466	38.0	31.0	40.0	2.0	41.0
19	31.701640446279956	38.0	31.0	40.0	2.0	41.0
20	31.573483967211395	38.0	30.0	40.0	2.0	41.0
21	31.517695586136128	38.0	30.0	40.0	2.0	41.0
22	30.767851790893896	38.0	29.0	40.0	2.0	41.0
23	30.870688706353683	38.0	30.0	40.0	2.0	41.0
24	30.516555164408683	38.0	29.0	40.0	2.0	41.0
25	30.03359714581312	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109552.0
3	6.0
4	911.0
5	5030.0
6	5850.0
7	5064.0
8	4395.0
9	4007.0
10	3709.0
11	3489.0
12	3363.0
13	3249.0
14	3237.0
15	3320.0
16	3451.0
17	3776.0
18	4375.0
19	5496.0
20	6856.0
21	8444.0
22	10121.0
23	11781.0
24	13452.0
25	15133.0
26	17063.0
27	18897.0
28	21202.0
29	24702.0
30	29291.0
31	34615.0
32	43100.0
33	54735.0
34	70260.0
35	92023.0
36	122147.0
37	166079.0
38	248961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01476057263704	18.170037934705555	12.62660782086756	25.188593671789842
2	16.067811771761697	20.363861845332032	39.23098882425543	24.337337558650844
3	18.687364355592212	24.576586219247936	29.928268823823796	26.807780601336056
4	13.563336155800169	16.505249788314988	35.364690939881456	34.56672311600339
5	13.597383265566709	37.01700403212833	34.40407988389638	14.981532818408589
6	32.31758631958779	34.755159025140244	17.79290072083269	15.134353934439277
7	28.239631055483073	32.29991728043119	21.296182623133202	18.164269040952536
8	25.692942812004176	34.06176499972061	21.448758045316232	18.796534142958983
9	27.147885326449106	16.13300628166126	19.34987049735957	37.36923789453007
10	15.835416855404274	28.45305153785519	32.95320520907329	22.758326397667243
11	34.73308168176407	23.289370515445412	22.51206183245018	19.46548597034034
12	23.42554020185599	25.389571902729795	29.234572918783446	21.950314976630768
13	28.25046801455989	22.291209623954217	25.41376212583178	24.044560235654117
14	22.710764460168118	21.599033321450257	26.155953972688113	29.53424824569351
15	24.53340603644695	28.249985817812657	23.427872781969402	23.78873536377099
16	23.782669083148427	27.604126086146447	25.146089872795947	23.46711495790918
17	22.975814887120613	27.31719298542795	26.066054874924305	23.640937252527134
18	22.75986467432496	27.129410469532665	26.83716459683873	23.27356025930364
19	24.014920254541913	26.617158292723204	27.18873444571932	22.179187007015567
20	24.790782312838765	25.77177468590885	26.545137080664926	22.89230592058746
21	25.054219004978577	25.855161369816383	26.23073542226464	22.859884202940403
22	23.96871668824302	25.106830460672757	27.525047054510416	23.399405796573806
23	24.271656393715816	25.69671607110649	26.492442839686923	23.53918469549077
24	23.91883081143786	25.690564695721385	26.560512790116764	23.830091702723983
25	23.443282956178084	25.649732604399972	27.24517458759322	23.66180985182873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	948.0
1	948.0
2	1008.0
3	1068.0
4	1068.0
5	1068.0
6	1418.5
7	1769.0
8	1769.0
9	1769.0
10	2183.5
11	2598.0
12	2598.0
13	2598.0
14	3691.5
15	4785.0
16	4785.0
17	4785.0
18	8117.0
19	11449.0
20	11449.0
21	11449.0
22	17345.5
23	23242.0
24	23242.0
25	23242.0
26	33145.5
27	43049.0
28	43049.0
29	43049.0
30	54885.0
31	66721.0
32	66721.0
33	66721.0
34	78755.0
35	90789.0
36	90789.0
37	90789.0
38	102289.5
39	113790.0
40	113790.0
41	113790.0
42	125017.0
43	136244.0
44	136244.0
45	136244.0
46	145312.5
47	154381.0
48	154381.0
49	154381.0
50	155108.5
51	155836.0
52	155836.0
53	155836.0
54	145081.5
55	134327.0
56	134327.0
57	134327.0
58	117818.0
59	101309.0
60	101309.0
61	101309.0
62	85105.5
63	68902.0
64	68902.0
65	68902.0
66	53812.0
67	38722.0
68	38722.0
69	38722.0
70	28559.0
71	18396.0
72	18396.0
73	18396.0
74	13083.5
75	7771.0
76	7771.0
77	7771.0
78	5537.5
79	3304.0
80	3304.0
81	3304.0
82	2256.5
83	1209.0
84	1209.0
85	1209.0
86	808.0
87	407.0
88	407.0
89	407.0
90	253.0
91	99.0
92	99.0
93	99.0
94	59.5
95	20.0
96	20.0
97	20.0
98	13.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03632078107458714
2	0.054100184397811606
3	0.017356084196481034
4	0.01202226319951369
5	0.01083696964463206
6	6.773106027895037E-4
7	0.003640544489993582
8	0.0016932765069737595
9	0.007196425154638478
10	0.004148527442085711
11	0.012191590850211067
12	0.008635710185566171
13	0.007535080456033229
14	0.016170790641599403
15	0.007027097503941102
16	0.030648304776225044
17	0.0386913681843504
18	0.024298517875073446
19	0.016678773593691527
20	0.016509445942994153
21	0.023959862573678693
22	0.005503148647664717
23	0.017694739497875783
24	0.01286890145300057
25	0.4692915839077774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1181142.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.107526323238865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54637028137074	43.31705173426663
2	12.567615593661898	14.60546107858112
3	5.327742035496382	9.287457317130967
4	2.6964061705132774	6.267259701249758
5	1.5086831106795118	4.383292178361781
6	0.9236602560597511	3.2202967585632924
7	0.6198583671242343	2.521290546904591
8	0.40457037141009133	1.8806866805051528
9	0.3011200867610463	1.5747609031131122
>10	1.062866599902043	9.761489863209308
>50	0.028068181958679488	1.100437547226248
>100	0.0110906024386598	1.1101900202912798
>500	0.0013488525856034722	0.47897964353630457
>1k	5.994900380459876E-4	0.49134602706033276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2216	0.18761503697269252	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1197	0.1013425989423795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	8.466382534868797E-5
13	0.0	0.0	0.0	0.0	8.466382534868797E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	965	0.0	14.083119	1
TACGAGA	55	1.9658326E-4	13.812947	4
AGCACCG	100	1.4484067E-7	12.345322	5
GTCTTAG	120	9.944415E-9	11.879555	1
CGTGCGC	65	8.043594E-4	11.688373	10
CTTAGAC	75	2.0794998E-4	11.396164	3
TCAACTC	195	0.0	11.200883	5
CTATACT	120	1.2805322E-7	11.079135	4
GTCCTAG	165	8.0035534E-11	10.94359	1
TAGACAG	150	2.1427695E-9	10.762588	5
GTATCAA	2185	0.0	10.6562605	1
GTATAAT	125	2.2255699E-7	10.644081	1
GTTATAT	90	9.4728064E-5	10.559604	1
TCCTATA	75	0.0026420683	10.135503	2
AAGGCGT	75	0.00265603	10.1290655	6
TACACAA	160	6.199116E-9	10.089927	5
GTCATAA	85	6.567243E-4	10.062682	1
GTACACG	85	6.567243E-4	10.062682	1
TCTATAC	105	4.1171377E-5	9.949031	3
GTGGTAT	335	0.0	9.92918	1
>>END_MODULE