FastQCFastQC Report
Mon 15 Aug 2016
SRR2921159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181142
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38760.3281569870515145No Hit
GGTATCAACGCAGAGTACTTTTTTT26880.22757636253727323No Hit
TATCAACGCAGAGTACTTTTTTTTT23500.1989599895694167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15990.13537745673255205No Hit
GGTATCAACGCAGAGTACATGGGGG15400.13038229103697946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC852.6907946E-713.4143673
GGTATCA11150.013.3126761
TCTTATA1302.6029738E-911.6945762
GTATGAG752.0425492E-411.418181
CTATTGG1104.862486E-711.2451771
GGGTTAT600.00580496811.1010081
TCCGGTA600.00586576111.0854842
ACGATTC600.005884290711.0807898
TAGATAG803.775757E-410.6854985
GTATTGA909.357276E-510.572391
CTAGACC909.491011E-510.5576043
TAGAGTG909.527779E-510.5535795
TATTAGG1002.3919025E-510.4520282
ATAAGAC1002.3919025E-510.4520283
TAGACTG1106.053584E-610.3616955
GTAAGGC750.002642506510.13533
CTCTAGA3850.010.1330181
GTATCAA22900.010.0553121
GTATTAG951.6168489E-410.0159471
GGGTAAA951.6168489E-410.0159471
Produced by FastQC (version 0.11.5)