##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181142
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.311585736516015	31.0	30.0	33.0	25.0	34.0
2	29.582796141361495	31.0	30.0	34.0	25.0	34.0
3	29.69623381439319	31.0	30.0	34.0	26.0	34.0
4	33.35645587067432	35.0	35.0	37.0	28.0	37.0
5	32.84420755506112	35.0	33.0	37.0	26.0	37.0
6	32.766641098191414	35.0	33.0	37.0	25.0	37.0
7	32.62451932113158	36.0	33.0	37.0	25.0	37.0
8	32.54995081031747	36.0	33.0	37.0	25.0	37.0
9	33.90720675414133	38.0	34.0	39.0	23.0	39.0
10	33.76956115352769	38.0	34.0	39.0	23.0	39.0
11	33.767997412673495	38.0	34.0	39.0	23.0	39.0
12	33.63868103919766	38.0	34.0	39.0	22.0	39.0
13	33.60694988409522	38.0	34.0	39.0	21.0	39.0
14	34.53210875576349	39.0	34.0	41.0	19.0	41.0
15	34.50962543030389	39.0	34.0	41.0	18.0	41.0
16	34.49883671903971	39.0	34.0	41.0	18.0	41.0
17	34.473965873705275	39.0	34.0	41.0	18.0	41.0
18	34.43314097712214	39.0	34.0	41.0	17.0	41.0
19	34.47504279756372	39.0	34.0	41.0	18.0	41.0
20	34.40476843597129	39.0	34.0	41.0	18.0	41.0
21	34.33176874584089	39.0	34.0	41.0	18.0	41.0
22	34.24201323803573	39.0	34.0	41.0	16.0	41.0
23	34.10579252960271	39.0	34.0	41.0	10.0	41.0
24	34.017780249961476	39.0	34.0	41.0	2.0	41.0
25	33.88120395346199	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46010.0
3	1.0
4	750.0
5	6365.0
6	6925.0
7	5362.0
8	4026.0
9	3579.0
10	3179.0
11	2880.0
12	2659.0
13	2482.0
14	2370.0
15	2307.0
16	2379.0
17	2463.0
18	2721.0
19	3222.0
20	4052.0
21	5254.0
22	6366.0
23	7872.0
24	9037.0
25	10662.0
26	11785.0
27	13145.0
28	14861.0
29	18207.0
30	22801.0
31	29131.0
32	37111.0
33	47479.0
34	62184.0
35	82801.0
36	111934.0
37	168716.0
38	418064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05995352203635	19.141225546946643	9.370767700018815	31.42805323099819
2	14.897192716879088	21.575559924208942	35.33004499035679	28.19720236855518
3	17.692316813502703	24.88222908454605	28.05683494793687	29.368619154014375
4	13.334011029505197	17.053885316865316	33.636517658220875	35.97558599540861
5	14.394862366666384	37.98654590743109	32.30880023044793	15.309791495454583
6	32.751784231030086	35.50113840247714	16.92456062067278	14.822516745820003
7	28.77665559535733	32.77138606979243	19.897708213568183	18.55425012128206
8	27.01868586761136	34.92256486372754	19.07069369452514	18.988055574135963
9	27.422443702789334	15.891315354123382	18.92549752696966	37.76074341611762
10	16.682922121133615	28.946561886716417	31.014729812334163	23.355786179815805
11	35.09278168954458	23.162570318665054	21.977355781117172	19.767292210673197
12	23.60664509432397	25.40498940855545	28.66827189279528	22.320093604325304
13	28.494600441108613	21.84148029616083	25.244281886183817	24.41963737654674
14	22.806051477203606	21.317665277878966	25.914473494712837	29.961809750204594
15	24.60654242567177	28.226140402429717	22.960731727351792	24.20658544454672
16	24.191572353988562	27.40894208649814	24.460138059028573	23.939347500484722
17	23.044223302532636	27.32753555457346	25.921946726134536	23.706294416759373
18	22.965775926302243	26.91517645465014	26.794350741719164	23.324696877328456
19	24.19043200102958	26.41817279702505	26.797661765428067	22.593733436517304
20	24.715402551090385	25.65474769333408	26.459223361797314	23.170626393778225
21	24.871644399732745	25.53698495807008	26.36753862946789	23.223832012729282
22	24.655417647357638	25.20073826980714	26.213488663494587	23.93035541934064
23	24.24582632711706	25.52455604719015	26.2543878810662	23.975229744626592
24	23.84948150355924	25.687228992786686	26.353607206029412	24.109682297624662
25	23.803444938576543	25.653061588010317	26.542584187414704	24.000909285998436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	739.0
1	739.0
2	813.5
3	888.0
4	888.0
5	888.0
6	1124.5
7	1361.0
8	1361.0
9	1361.0
10	1759.5
11	2158.0
12	2158.0
13	2158.0
14	2824.5
15	3491.0
16	3491.0
17	3491.0
18	6345.5
19	9200.0
20	9200.0
21	9200.0
22	14715.0
23	20230.0
24	20230.0
25	20230.0
26	29769.0
27	39308.0
28	39308.0
29	39308.0
30	50620.0
31	61932.0
32	61932.0
33	61932.0
34	73188.5
35	84445.0
36	84445.0
37	84445.0
38	95865.5
39	107286.0
40	107286.0
41	107286.0
42	119061.5
43	130837.0
44	130837.0
45	130837.0
46	141816.5
47	152796.0
48	152796.0
49	152796.0
50	157219.0
51	161642.0
52	161642.0
53	161642.0
54	151802.5
55	141963.0
56	141963.0
57	141963.0
58	125438.0
59	108913.0
60	108913.0
61	108913.0
62	92174.0
63	75435.0
64	75435.0
65	75435.0
66	59717.0
67	43999.0
68	43999.0
69	43999.0
70	32130.5
71	20262.0
72	20262.0
73	20262.0
74	14393.0
75	8524.0
76	8524.0
77	8524.0
78	6093.0
79	3662.0
80	3662.0
81	3662.0
82	2507.5
83	1353.0
84	1353.0
85	1353.0
86	943.5
87	534.0
88	534.0
89	534.0
90	344.0
91	154.0
92	154.0
93	154.0
94	92.0
95	30.0
96	30.0
97	30.0
98	15.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10396717752818882
2	0.0
3	0.038945359660396466
4	0.05689409063431831
5	0.07044030269010838
6	0.008551046360217486
7	9.313020788355677E-4
8	0.007535080456033229
9	0.0
10	0.0
11	0.007619744281381917
12	0.0
13	0.0022859232844145753
14	0.06231257545663434
15	0.02243591371740231
16	0.004487182743480462
17	0.0
18	0.008635710185566171
19	0.006773106027895038
20	0.0
21	0.020573309559731173
22	1.6932765069737593E-4
23	0.004063863616737022
24	0.011090961120678124
25	0.18583709664037007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1181142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.19639048213025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.72291189434574	30.36161301493839
2	14.922807403095337	13.787596757660323
3	7.070004211584996	9.798260258060576
4	3.9725223853841625	7.340647812568411
5	2.348972507640484	5.425702559737424
6	1.5337460408582704	4.251211860234597
7	1.0234882248039474	3.3097023180833824
8	0.7231123333839151	2.6724143772358153
9	0.5214413502712636	2.1679837407594484
>10	2.0772641348069296	15.39183266880862
>50	0.05626877549172887	1.7329966432135933
>100	0.02395903750054925	1.8902791535906434
>500	0.0023959005696814802	0.7565537547823941
>1k	0.001105800262929914	1.1132050803263338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3876	0.3281569870515145	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2688	0.22757636253727323	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2350	0.1989599895694167	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1599	0.13537745673255205	No Hit
GGTATCAACGCAGAGTACATGGGGG	1540	0.13038229103697946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.466382534868797E-5	0.0
10	0.0	0.0	0.0	8.466382534868797E-5	0.0
11	0.0	0.0	0.0	8.466382534868797E-5	0.0
12	0.0	0.0	0.0	8.466382534868797E-5	0.0
13	0.0	0.0	0.0	8.466382534868797E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAAGAC	85	2.6907946E-7	13.414367	3
GGTATCA	1115	0.0	13.312676	1
TCTTATA	130	2.6029738E-9	11.694576	2
GTATGAG	75	2.0425492E-4	11.41818	1
CTATTGG	110	4.862486E-7	11.245177	1
GGGTTAT	60	0.005804968	11.101008	1
TCCGGTA	60	0.005865761	11.085484	2
ACGATTC	60	0.0058842907	11.080789	8
TAGATAG	80	3.775757E-4	10.685498	5
GTATTGA	90	9.357276E-5	10.57239	1
CTAGACC	90	9.491011E-5	10.557604	3
TAGAGTG	90	9.527779E-5	10.553579	5
TATTAGG	100	2.3919025E-5	10.452028	2
ATAAGAC	100	2.3919025E-5	10.452028	3
TAGACTG	110	6.053584E-6	10.361695	5
GTAAGGC	75	0.0026425065	10.1353	3
CTCTAGA	385	0.0	10.133018	1
GTATCAA	2290	0.0	10.055312	1
GTATTAG	95	1.6168489E-4	10.015947	1
GGGTAAA	95	1.6168489E-4	10.015947	1
>>END_MODULE