FastQCFastQC Report
Mon 15 Aug 2016
SRR2921158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036612
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22910.11249074443242012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA550.00307159412.08954911
CGACAGT550.003078380812.0859876
GGTATCA13450.012.0084441
GCGCAAA802.8706849E-511.87366511
GTTCCGT802.8793555E-511.8701676
GAAGCGT1053.4842851E-610.8527236
TATACCG700.00149712910.8524565
TCGTGTC803.7756778E-410.68629814
TGTGCGA856.607599E-410.056704510
ATACCGT800.00453165839.4961336
AGCCTCG2451.8189894E-129.3050772
GTCTAAG1951.6789272E-99.2571881
GTATAGA1651.0642907E-79.2128951
TTACACT1554.288795E-79.1893544
GTATCAA25550.09.073171
ACCGTCT1054.5079732E-49.0446028
GTCTAAT950.00182256949.0007561
GTATAAC1701.6826925E-78.9419281
GTCTTAT1606.7324436E-78.9069991
GTATTAA2607.2759576E-128.7699681
Produced by FastQC (version 0.11.5)