##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921158_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2036612
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.905397788091204	30.0	26.0	31.0	2.0	34.0
2	25.532936072261187	31.0	25.0	31.0	2.0	34.0
3	25.332952472046713	30.0	25.0	31.0	2.0	34.0
4	29.0741496171092	35.0	28.0	37.0	2.0	37.0
5	28.65627915381035	35.0	28.0	37.0	2.0	37.0
6	29.122232904451117	35.0	29.0	37.0	2.0	37.0
7	28.923920707527994	35.0	29.0	37.0	2.0	37.0
8	28.64079608683441	35.0	28.0	37.0	2.0	37.0
9	30.22273314701082	37.0	29.0	39.0	2.0	39.0
10	29.609282966023965	35.0	27.0	39.0	2.0	39.0
11	30.032682710305153	37.0	29.0	39.0	2.0	39.0
12	29.753963936184213	37.0	27.0	39.0	2.0	39.0
13	29.726133892955556	37.0	27.0	39.0	2.0	39.0
14	30.7273280330274	37.0	29.0	40.0	2.0	41.0
15	30.411626269510343	37.0	27.0	40.0	2.0	41.0
16	30.312818543738327	37.0	27.0	40.0	2.0	41.0
17	30.57643625786355	37.0	28.0	40.0	2.0	41.0
18	30.604677768764986	38.0	29.0	40.0	2.0	41.0
19	30.3189979240032	37.0	27.0	40.0	2.0	41.0
20	30.223163273122225	37.0	27.0	40.0	2.0	41.0
21	30.148725432237462	37.0	27.0	40.0	2.0	41.0
22	29.437332196805283	37.0	25.0	40.0	2.0	41.0
23	29.503851985552476	37.0	27.0	40.0	2.0	41.0
24	29.169357246250144	37.0	25.0	40.0	2.0	41.0
25	28.677781040276695	37.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	244247.0
3	10.0
4	1876.0
5	9908.0
6	11339.0
7	9632.0
8	8117.0
9	7488.0
10	7087.0
11	6553.0
12	6533.0
13	6265.0
14	6111.0
15	6252.0
16	6642.0
17	7253.0
18	8252.0
19	10099.0
20	12645.0
21	15749.0
22	18811.0
23	21762.0
24	24805.0
25	28009.0
26	30718.0
27	34601.0
28	37672.0
29	43688.0
30	51843.0
31	61665.0
32	76741.0
33	96925.0
34	122720.0
35	159998.0
36	207979.0
37	269920.0
38	356697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.214343041994894	17.707820664330615	11.800940511239668	26.276895782434817
2	16.03903817452418	20.72131997999554	38.01761296477702	25.222028880703263
3	19.67741555288405	24.082848140552688	29.16774705544948	27.07198925111378
4	13.903877241950976	16.176912545497043	34.22404090363564	35.69516930891634
5	14.19939324834096	36.3534402072659	33.519984600411114	15.92718194398203
6	33.06453474012726	34.10706017353492	17.802869792835388	15.025535293502431
7	28.559159827432296	31.216910692382253	20.875909756478407	19.348019723707043
8	26.843117546572337	33.663775684818	20.578985280592843	18.91412148801682
9	27.259759180973713	15.894095018480536	18.958716247304768	37.88742955324098
10	16.068055343456162	28.045945066066164	32.67045384796312	23.215545742514557
11	35.34095406835876	22.712281732137612	21.961339125327665	19.98542507417596
12	23.603382406379826	24.787568379803773	29.009192602704747	22.599856611111658
13	28.727443971776083	21.640774119794994	24.92230271715832	24.709479191270603
14	23.123036555367356	20.8985891436398	25.5633186185993	30.41505568239354
15	24.762896682174016	27.706444633662237	23.12706302229267	24.403595661871076
16	24.523722867049095	26.841268461310637	24.626181329867624	24.008827341772648
17	23.805025793071877	26.949904166285982	25.324022088418513	23.921047952223624
18	23.781520363982114	26.652830055241672	26.030722777956765	23.53492680281945
19	24.574858662727426	26.073878436194914	26.423833058974672	22.92742984210299
20	25.01893152533583	25.50324463263019	25.99796885113388	23.4798549909001
21	25.324096174889156	25.313045767350122	25.592547408703986	23.770310649056736
22	24.432730245793568	24.684686454223375	26.989562796316164	23.893020503666893
23	24.985758231742054	25.15479328810017	25.749018791276573	24.110429688881204
24	24.385763140681256	25.57102224177793	26.087731566510243	23.955483051030573
25	24.113995766497318	25.206359824049667	26.52495137292857	24.154693036524442
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	913.0
1	913.0
2	988.5
3	1064.0
4	1064.0
5	1064.0
6	1425.0
7	1786.0
8	1786.0
9	1786.0
10	2398.0
11	3010.0
12	3010.0
13	3010.0
14	4515.0
15	6020.0
16	6020.0
17	6020.0
18	10250.0
19	14480.0
20	14480.0
21	14480.0
22	22667.0
23	30854.0
24	30854.0
25	30854.0
26	44641.5
27	58429.0
28	58429.0
29	58429.0
30	77819.0
31	97209.0
32	97209.0
33	97209.0
34	118984.5
35	140760.0
36	140760.0
37	140760.0
38	166505.0
39	192250.0
40	192250.0
41	192250.0
42	214846.0
43	237442.0
44	237442.0
45	237442.0
46	255288.0
47	273134.0
48	273134.0
49	273134.0
50	277689.0
51	282244.0
52	282244.0
53	282244.0
54	264787.5
55	247331.0
56	247331.0
57	247331.0
58	217810.0
59	188289.0
60	188289.0
61	188289.0
62	157402.5
63	126516.0
64	126516.0
65	126516.0
66	99767.0
67	73018.0
68	73018.0
69	73018.0
70	54880.0
71	36742.0
72	36742.0
73	36742.0
74	26070.0
75	15398.0
76	15398.0
77	15398.0
78	10866.5
79	6335.0
80	6335.0
81	6335.0
82	4291.0
83	2247.0
84	2247.0
85	2247.0
86	1553.5
87	860.0
88	860.0
89	860.0
90	544.0
91	228.0
92	228.0
93	228.0
94	134.5
95	41.0
96	41.0
97	41.0
98	26.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03427260568041434
2	0.052341830451750254
3	0.01512315551513985
4	0.010605849322305868
5	0.009525623928367309
6	9.32921931128757E-4
7	0.0038298900330548972
8	0.0024059565592267945
9	0.007119667369140514
10	0.004566407347103916
11	0.01168607471624443
12	0.009427421619827438
13	0.008985511231398027
14	0.016252482063348343
15	0.007757982374649663
16	0.0326522675895065
17	0.038986316490328056
18	0.02297934019832938
19	0.017087201685937234
20	0.01600697629199867
21	0.023961363283728075
22	0.007217869677680383
23	0.017087201685937234
24	0.01296270472726273
25	0.4639568066966119
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2036612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.91096242847433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1589584436504	52.031951398414265
2	10.830401387386067	14.060235550838266
3	3.944372339267938	7.6809901415440365
4	1.7884050696962421	4.643483771437832
5	1.0135704309960085	3.2895916082497227
6	0.5658438058014399	2.2037679611257315
7	0.37855293487176267	1.720056473285477
8	0.264304608657765	1.3725013217805437
9	0.1984965810890963	1.1596143705529443
>10	0.8189674186994573	8.892752064092392
>50	0.028423398400206412	1.253812874011613
>100	0.008395376686612372	0.8954218916099947
>500	9.234386802168998E-4	0.4142942400788145
>1k	3.8476611675703E-4	0.3815263329782872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2291	0.11249074443242012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.910115426993458E-5
2	0.0	0.0	0.0	0.0	4.910115426993458E-5
3	0.0	0.0	0.0	0.0	4.910115426993458E-5
4	0.0	0.0	0.0	0.0	4.910115426993458E-5
5	0.0	0.0	0.0	0.0	4.910115426993458E-5
6	0.0	0.0	0.0	0.0	4.910115426993458E-5
7	0.0	0.0	0.0	0.0	9.820230853986916E-5
8	0.0	0.0	0.0	0.0	9.820230853986916E-5
9	0.0	0.0	0.0	0.0	9.820230853986916E-5
10	0.0	0.0	0.0	0.0	9.820230853986916E-5
11	0.0	0.0	0.0	0.0	9.820230853986916E-5
12	0.0	0.0	0.0	0.0	9.820230853986916E-5
13	0.0	0.0	0.0	0.0	9.820230853986916E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCGAA	55	0.003071594	12.089549	11
CGACAGT	55	0.0030783808	12.085987	6
GGTATCA	1345	0.0	12.008444	1
GCGCAAA	80	2.8706849E-5	11.873665	11
GTTCCGT	80	2.8793555E-5	11.870167	6
GAAGCGT	105	3.4842851E-6	10.852723	6
TATACCG	70	0.001497129	10.852456	5
TCGTGTC	80	3.7756778E-4	10.686298	14
TGTGCGA	85	6.607599E-4	10.0567045	10
ATACCGT	80	0.0045316583	9.496133	6
AGCCTCG	245	1.8189894E-12	9.305077	2
GTCTAAG	195	1.6789272E-9	9.257188	1
GTATAGA	165	1.0642907E-7	9.212895	1
TTACACT	155	4.288795E-7	9.189354	4
GTATCAA	2555	0.0	9.07317	1
ACCGTCT	105	4.5079732E-4	9.044602	8
GTCTAAT	95	0.0018225694	9.000756	1
GTATAAC	170	1.6826925E-7	8.941928	1
GTCTTAT	160	6.7324436E-7	8.906999	1
GTATTAA	260	7.2759576E-12	8.769968	1
>>END_MODULE