FastQCFastQC Report
Mon 15 Aug 2016
SRR2921158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036612
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41270.20264046367202No Hit
GGTATCAACGCAGAGTACTTTTTTT30510.1498076216775704No Hit
TATCAACGCAGAGTACTTTTTTTTT24030.11799007371065279No Hit
GGTATCAACGCAGAGTACATGGGGG23260.11420928483186782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT604.0396297E-412.6863861
CCGTGCG658.0232025E-411.6926719
GGTATCA14300.011.5108991
GTCTAAG1752.2919266E-1010.3303421
GTTCTAA1303.7612335E-710.2466971
CGTGCGC750.002646952910.13389710
GTATCAA28550.09.8313931
GATTTCG900.00110478059.50847219
CCCGTAC900.00110951129.5040313
TCTACAC2002.6193447E-109.504033
GAACCGC1304.277412E-69.4932986
CCACGCA1451.7056609E-69.1726999
CTACACT2800.09.1646014
GTATTAG1356.7139845E-69.162391
GTCTAGT1356.7139845E-69.162391
AAGACCG1356.8557674E-69.1473085
TGGACCG1151.1118475E-49.08612255
GTAATAC1054.4739578E-49.0514583
AGGCCGT1054.5247807E-49.0412356
CTAGACA1901.0501026E-89.00381854
Produced by FastQC (version 0.11.5)