##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2036612
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.132222043275792	31.0	30.0	33.0	25.0	34.0
2	29.41850386818893	31.0	30.0	34.0	25.0	34.0
3	29.54453278287666	31.0	30.0	34.0	25.0	34.0
4	33.19698155564241	35.0	35.0	37.0	28.0	37.0
5	32.636613650513695	35.0	33.0	37.0	25.0	37.0
6	32.55413549561723	35.0	33.0	37.0	25.0	37.0
7	32.409178576969985	35.0	33.0	37.0	23.0	37.0
8	32.33796422686304	36.0	33.0	37.0	22.0	37.0
9	33.69396183465481	38.0	34.0	39.0	22.0	39.0
10	33.55446692840855	38.0	34.0	39.0	19.0	39.0
11	33.55387476848806	38.0	34.0	39.0	19.0	39.0
12	33.42270692699444	38.0	34.0	39.0	18.0	39.0
13	33.39006742570504	38.0	34.0	39.0	17.0	39.0
14	34.3181941381078	39.0	34.0	41.0	17.0	41.0
15	34.28690246350311	39.0	34.0	41.0	17.0	41.0
16	34.26241326281098	39.0	34.0	41.0	17.0	41.0
17	34.23035217311889	39.0	34.0	41.0	17.0	41.0
18	34.183015714333415	39.0	34.0	41.0	17.0	41.0
19	34.220882524506386	39.0	34.0	41.0	16.0	41.0
20	34.15394390291327	39.0	34.0	41.0	13.0	41.0
21	34.08095012697559	39.0	34.0	41.0	10.0	41.0
22	33.99407152663345	39.0	34.0	41.0	10.0	41.0
23	33.86220743077228	39.0	34.0	41.0	2.0	41.0
24	33.771701728164224	39.0	34.0	41.0	2.0	41.0
25	33.632396352373455	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87634.0
3	5.0
4	1491.0
5	12116.0
6	13066.0
7	9767.0
8	7510.0
9	6447.0
10	5772.0
11	5050.0
12	4780.0
13	4460.0
14	4281.0
15	4129.0
16	4181.0
17	4460.0
18	4813.0
19	5727.0
20	7118.0
21	9222.0
22	11353.0
23	13598.0
24	15720.0
25	18109.0
26	20331.0
27	22770.0
28	25883.0
29	31447.0
30	39218.0
31	49925.0
32	63006.0
33	80693.0
34	105930.0
35	140451.0
36	190701.0
37	289179.0
38	716269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.016622293040896	18.59037843486022	8.936227309611589	32.45677196248729
2	14.914426508338357	21.25942496656211	34.633057253909925	29.1930912711896
3	18.05446052562419	24.211762660060625	27.64019289556042	30.093583918754756
4	13.554545816304422	16.794079515246555	32.70130468595324	36.95006998249578
5	14.84194228226372	37.12868732958546	32.44874603891385	15.58062434923697
6	33.5870612978674	34.94855956468983	16.55973950530167	14.9046396321411
7	29.16533065295839	31.785079628053786	20.07835873846261	18.971230980525217
8	27.52534689161445	34.59149114411746	18.86323358915521	19.01992837511288
9	27.11235326668968	15.731293727839937	18.590466575960065	38.565886429510314
10	16.638310680460197	28.742020429076398	31.061466448853416	23.55820244160999
11	35.550670116482856	22.622993001993187	21.705360882757482	20.120975998766475
12	23.773710345274576	24.920124658071668	28.362804678949487	22.943360317704265
13	28.87667947623813	21.231889253021606	24.950429610046264	24.941001660693995
14	22.927178203277684	20.68414934791217	25.37001427685705	31.0186581719531
15	24.8199656310301	27.805272626279443	22.522971172400315	24.85179057029014
16	24.88681428114056	26.799088228881658	23.98928344846686	24.324814041510923
17	23.741964501411182	26.972171101862525	25.25989547223101	24.025968924495285
18	23.7520796217691	26.4718909777316	26.074479431632252	23.701549968867056
19	24.689510228533827	26.01141195996975	26.0404329080856	23.258644903410822
20	24.93152702828776	25.45019321702667	25.826708632651957	23.791571122033613
21	25.13541438520641	25.174258547925643	25.721612577847925	23.96871448902002
22	24.985367367461738	24.95654402752899	25.618498790107164	24.439589814902106
23	24.72278064929874	25.06616828891506	25.687683712141673	24.52336734964453
24	24.34551425843937	25.558188732316907	25.85528047424681	24.241016534996916
25	24.383389020352773	25.228752897684263	25.737191042792517	24.650667039170447
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	661.0
1	661.0
2	689.5
3	718.0
4	718.0
5	718.0
6	998.5
7	1279.0
8	1279.0
9	1279.0
10	1783.5
11	2288.0
12	2288.0
13	2288.0
14	3345.5
15	4403.0
16	4403.0
17	4403.0
18	8252.0
19	12101.0
20	12101.0
21	12101.0
22	19519.0
23	26937.0
24	26937.0
25	26937.0
26	40420.0
27	53903.0
28	53903.0
29	53903.0
30	71411.0
31	88919.0
32	88919.0
33	88919.0
34	110231.5
35	131544.0
36	131544.0
37	131544.0
38	157133.5
39	182723.0
40	182723.0
41	182723.0
42	206100.5
43	229478.0
44	229478.0
45	229478.0
46	250462.0
47	271446.0
48	271446.0
49	271446.0
50	280931.5
51	290417.0
52	290417.0
53	290417.0
54	275440.5
55	260464.0
56	260464.0
57	260464.0
58	229735.5
59	199007.0
60	199007.0
61	199007.0
62	168358.0
63	137709.0
64	137709.0
65	137709.0
66	108339.5
67	78970.0
68	78970.0
69	78970.0
70	58734.5
71	38499.0
72	38499.0
73	38499.0
74	27169.0
75	15839.0
76	15839.0
77	15839.0
78	11007.0
79	6175.0
80	6175.0
81	6175.0
82	4194.0
83	2213.0
84	2213.0
85	2213.0
86	1461.0
87	709.0
88	709.0
89	709.0
90	445.5
91	182.0
92	182.0
93	182.0
94	103.0
95	24.0
96	24.0
97	24.0
98	14.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09795680276851948
2	0.0
3	0.04414193768867119
4	0.05415857315973784
5	0.0737008325591718
6	0.008789106614318289
7	0.0039771934958647
8	0.008298095071618944
9	4.419103884294112E-4
10	2.4550577134967286E-4
11	0.008592701997238552
12	4.910115426993457E-5
13	0.005597531586772542
14	0.05646632741042476
15	0.023224845969679057
16	0.006677756980711103
17	0.0011784277024784297
18	0.009034612385667962
19	0.007463375449030056
20	0.002062248479337252
21	0.013257311652882336
22	0.0033388784903555513
23	0.006775959289250972
24	0.010016635471066654
25	0.17298336649297952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2036612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.38821847228995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09762149832434	37.24677727914394
2	14.483641171814929	15.175443159665884
3	5.8392372280639835	9.177217068460344
4	2.9664834970988867	6.216351421618367
5	1.6285251583784177	4.265776589237457
6	0.9758798278897369	3.067476337571295
7	0.6383329827885543	2.3408789432276698
8	0.43801470085126826	1.8357447873816775
9	0.3185999148572744	1.5021793750316281
>10	1.5265359542095864	13.66665711700904
>50	0.06333391573363864	2.2564791602350214
>100	0.021623675713824345	1.8315339145878136
>500	0.001321158254755801	0.4821188694993429
>1k	8.493160209144369E-4	0.9353659773305489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4127	0.20264046367202	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3051	0.1498076216775704	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2403	0.11799007371065279	No Hit
GGTATCAACGCAGAGTACATGGGGG	2326	0.11420928483186782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.820230853986916E-5
2	0.0	0.0	0.0	0.0	9.820230853986916E-5
3	0.0	0.0	0.0	0.0	9.820230853986916E-5
4	0.0	0.0	0.0	0.0	9.820230853986916E-5
5	0.0	0.0	0.0	0.0	9.820230853986916E-5
6	0.0	0.0	0.0	0.0	2.946069256196075E-4
7	0.0	0.0	0.0	0.0	2.946069256196075E-4
8	0.0	0.0	0.0	0.0	2.946069256196075E-4
9	0.0	0.0	0.0	0.0	3.4370807988954205E-4
10	0.0	0.0	0.0	0.0	3.4370807988954205E-4
11	0.0	0.0	0.0	0.0	3.4370807988954205E-4
12	0.0	0.0	0.0	0.0	3.4370807988954205E-4
13	0.0	0.0	0.0	0.0	3.928092341594766E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAT	60	4.0396297E-4	12.686386	1
CCGTGCG	65	8.0232025E-4	11.692671	9
GGTATCA	1430	0.0	11.510899	1
GTCTAAG	175	2.2919266E-10	10.330342	1
GTTCTAA	130	3.7612335E-7	10.246697	1
CGTGCGC	75	0.0026469529	10.133897	10
GTATCAA	2855	0.0	9.831393	1
GATTTCG	90	0.0011047805	9.508472	19
CCCGTAC	90	0.0011095112	9.504031	3
TCTACAC	200	2.6193447E-10	9.50403	3
GAACCGC	130	4.277412E-6	9.493298	6
CCACGCA	145	1.7056609E-6	9.172699	9
CTACACT	280	0.0	9.164601	4
GTATTAG	135	6.7139845E-6	9.16239	1
GTCTAGT	135	6.7139845E-6	9.16239	1
AAGACCG	135	6.8557674E-6	9.147308	5
TGGACCG	115	1.1118475E-4	9.0861225	5
GTAATAC	105	4.4739578E-4	9.051458	3
AGGCCGT	105	4.5247807E-4	9.041235	6
CTAGACA	190	1.0501026E-8	9.0038185	4
>>END_MODULE