FastQCFastQC Report
Mon 15 Aug 2016
SRR2921157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526577
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34170.13524226651315197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG551.1326736E-515.5399325
GGTATCA13750.013.3369371
TAATACG658.0504274E-411.6881544
ACCGTAT1104.9964547E-711.2230628
TACCGAT855.349302E-511.1716177
ACCGACC951.3666866E-510.9958748
ATACCGA700.001497378810.85242756
GTATCAA31000.010.8196821
TTATACT3350.010.488814
CCGTATA951.6506537E-49.9964489
ACTATAC1951.6552804E-109.7403213
ACGTAAC1502.6428643E-79.5017021
CGCTCGT900.00111663599.49756410
CTTATAC2401.8189894E-129.4968143
TAGACCG900.00111764729.4966255
TACGCTG1002.766223E-49.4966255
TAGGGTA1002.766223E-49.4966254
GACCGTA1002.7684236E-49.4958737
TAGACTG2650.09.3174445
ACAGCGC1451.7157872E-69.1689738
Produced by FastQC (version 0.11.5)