##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921157_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2526577
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.222175298833164	31.0	28.0	31.0	10.0	34.0
2	26.982150949684097	31.0	27.0	31.0	10.0	34.0
3	26.872942720526627	31.0	27.0	31.0	10.0	34.0
4	30.731376087093327	35.0	31.0	37.0	12.0	37.0
5	30.675023955335618	35.0	31.0	37.0	10.0	37.0
6	30.950801420261485	35.0	32.0	37.0	11.0	37.0
7	30.81159806330858	35.0	32.0	37.0	10.0	37.0
8	30.52689825008302	35.0	31.0	37.0	10.0	37.0
9	32.17300679931781	37.0	32.0	39.0	10.0	39.0
10	31.58210060488954	37.0	31.0	39.0	8.0	39.0
11	32.040012237901315	37.0	32.0	39.0	8.0	39.0
12	31.729507946917906	37.0	32.0	39.0	7.0	39.0
13	31.738991133062637	37.0	32.0	39.0	2.0	39.0
14	32.86353829707149	38.0	32.0	40.0	2.0	41.0
15	32.5177503001096	38.0	32.0	40.0	2.0	41.0
16	32.450603326160255	38.0	31.0	40.0	2.0	41.0
17	32.774018365559414	38.0	32.0	40.0	2.0	41.0
18	32.81511309570221	38.0	32.0	40.0	2.0	41.0
19	32.51708101514421	38.0	32.0	40.0	2.0	41.0
20	32.408073056946215	38.0	32.0	40.0	2.0	41.0
21	32.357100139833456	38.0	32.0	40.0	2.0	41.0
22	31.586504982828547	38.0	30.0	40.0	2.0	41.0
23	31.695817305389863	38.0	31.0	40.0	2.0	41.0
24	31.345903964138042	38.0	30.0	40.0	2.0	41.0
25	30.855155809619102	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	193705.0
3	11.0
4	1475.0
5	8670.0
6	10913.0
7	9498.0
8	7838.0
9	7178.0
10	6974.0
11	6741.0
12	6448.0
13	6310.0
14	6470.0
15	6604.0
16	6944.0
17	7461.0
18	8776.0
19	10694.0
20	13763.0
21	17109.0
22	20793.0
23	24198.0
24	28212.0
25	31547.0
26	36101.0
27	40399.0
28	44866.0
29	53248.0
30	62876.0
31	75303.0
32	94267.0
33	120632.0
34	157066.0
35	205255.0
36	275963.0
37	375179.0
38	537090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.365332260613826	18.16699582051164	12.216807593080325	25.250864325794204
2	16.141491706252637	19.82060080907847	38.86206261546595	25.17584486920294
3	18.78271733175414	23.544207389485486	29.974815769555978	27.698259509204398
4	13.936004382697528	15.768725621442917	34.976000456003206	35.31926953985634
5	13.702805897268835	36.80405744002546	34.72380005399153	14.76933660871417
6	33.54104650550689	34.62425803295467	17.157274255400917	14.677421206137511
7	29.48070705123664	31.905491129403487	20.38826151507247	18.225540304287406
8	26.502367477278117	34.28941444962634	20.42858007962703	18.77963799346851
9	27.03740602776033	15.889219925024076	19.133308080118397	37.9400659670972
10	15.811042944785274	28.341025133584008	33.065624381555516	22.7823075400752
11	35.73294087563561	22.62635266884165	22.003505519134293	19.637200936388442
12	23.17989266779662	25.008965461006778	29.272843717567	22.538298153629608
13	28.618921164387874	21.513579155235753	25.327411357385127	24.54008832299125
14	22.54673105294847	20.903360803986683	25.947872129446036	30.602036013618815
15	24.51758793770946	28.200033327805084	23.114099442729017	24.168279291756434
16	24.135323461873465	27.381582073006573	24.865547549291314	23.61754691582865
17	23.330939194143834	27.56258681064233	25.734277474133005	23.37219652108083
18	23.3597239613416	27.124495699366157	26.403085007539516	23.112695331752718
19	24.283377590299253	26.49780931977281	26.759392531577625	22.459420558350317
20	24.69635996139629	25.92604174996101	26.254204962271128	23.123393326371573
21	24.983956931338042	25.685853556573147	25.922273559162207	23.407915952926608
22	24.069739182613333	25.04541960591219	27.333974023547743	23.550867187926734
23	24.48241204438781	25.284014774824843	26.21854500989435	24.015028170892993
24	23.929730671184508	25.73788337001441	26.479527209968808	23.852858748832276
25	23.54830038230562	25.419347193469548	27.13566640235857	23.89668602186626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1096.0
1	1096.0
2	1175.0
3	1254.0
4	1254.0
5	1254.0
6	1840.5
7	2427.0
8	2427.0
9	2427.0
10	3163.5
11	3900.0
12	3900.0
13	3900.0
14	5926.0
15	7952.0
16	7952.0
17	7952.0
18	13865.5
19	19779.0
20	19779.0
21	19779.0
22	30996.5
23	42214.0
24	42214.0
25	42214.0
26	61969.0
27	81724.0
28	81724.0
29	81724.0
30	106499.0
31	131274.0
32	131274.0
33	131274.0
34	159583.0
35	187892.0
36	187892.0
37	187892.0
38	218363.5
39	248835.0
40	248835.0
41	248835.0
42	273294.5
43	297754.0
44	297754.0
45	297754.0
46	318277.5
47	338801.0
48	338801.0
49	338801.0
50	337523.5
51	336246.0
52	336246.0
53	336246.0
54	314792.0
55	293338.0
56	293338.0
57	293338.0
58	257554.5
59	221771.0
60	221771.0
61	221771.0
62	186746.0
63	151721.0
64	151721.0
65	151721.0
66	118910.5
67	86100.0
68	86100.0
69	86100.0
70	64352.0
71	42604.0
72	42604.0
73	42604.0
74	30407.5
75	18211.0
76	18211.0
77	18211.0
78	12880.5
79	7550.0
80	7550.0
81	7550.0
82	5205.0
83	2860.0
84	2860.0
85	2860.0
86	1896.5
87	933.0
88	933.0
89	933.0
90	605.5
91	278.0
92	278.0
93	278.0
94	165.5
95	53.0
96	53.0
97	53.0
98	31.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03597753007329679
2	0.058656435169005344
3	0.015554641714857691
4	0.01104260824031882
5	0.010092706456205372
6	6.332678560756312E-4
7	0.0033246562443970634
8	0.0018998035682268935
9	0.006570154006784674
10	0.003047601557363975
11	0.01088429127629991
12	0.008509536816016294
13	0.006649312488794127
14	0.014486002207730065
15	0.006253520078746858
16	0.03075307026067284
17	0.038193967569561504
18	0.02192689951661873
19	0.016662860462990044
20	0.015633800196867144
21	0.022322691926666
22	0.005343197535638138
23	0.015356745509834057
24	0.011755034578403904
25	0.46842031729094347
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2526577.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.144642153309604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.21024241542392	36.42022365987286
2	13.422188239131325	13.729460288094646
3	5.83044076753191	8.945874199544622
4	3.1300768311680085	6.403466377698123
5	1.8028623927900347	4.610337596545292
6	1.142648831230937	3.5064219348122245
7	0.7568178661085767	2.7095025256148175
8	0.5390786293252731	2.2056786871470178
9	0.39722000724614565	1.828410761406525
>10	1.6736794468996754	14.18681451174033
>50	0.06792423751795976	2.3612524905589902
>100	0.025302275801887666	2.232993504767719
>500	9.587746259662074E-4	0.3396981726304334
>1k	5.592851984733395E-4	0.5198652895663712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3417	0.13524226651315197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	7.915848200945389E-5
5	0.0	0.0	0.0	0.0	7.915848200945389E-5
6	0.0	0.0	0.0	0.0	1.5831696401890778E-4
7	0.0	0.0	0.0	0.0	1.5831696401890778E-4
8	0.0	0.0	0.0	0.0	1.5831696401890778E-4
9	0.0	0.0	0.0	0.0	1.5831696401890778E-4
10	0.0	0.0	0.0	0.0	1.5831696401890778E-4
11	0.0	0.0	0.0	0.0	1.5831696401890778E-4
12	0.0	0.0	0.0	0.0	1.5831696401890778E-4
13	0.0	0.0	0.0	3.9579241004726946E-5	1.9789620502363474E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	55	1.1326736E-5	15.539932	5
GGTATCA	1375	0.0	13.336937	1
TAATACG	65	8.0504274E-4	11.688154	4
ACCGTAT	110	4.9964547E-7	11.223062	8
TACCGAT	85	5.349302E-5	11.171617	7
ACCGACC	95	1.3666866E-5	10.995874	8
ATACCGA	70	0.0014973788	10.8524275	6
GTATCAA	3100	0.0	10.819682	1
TTATACT	335	0.0	10.48881	4
CCGTATA	95	1.6506537E-4	9.996448	9
ACTATAC	195	1.6552804E-10	9.740321	3
ACGTAAC	150	2.6428643E-7	9.501702	1
CGCTCGT	90	0.0011166359	9.497564	10
CTTATAC	240	1.8189894E-12	9.496814	3
TAGACCG	90	0.0011176472	9.496625	5
TACGCTG	100	2.766223E-4	9.496625	5
TAGGGTA	100	2.766223E-4	9.496625	4
GACCGTA	100	2.7684236E-4	9.495873	7
TAGACTG	265	0.0	9.317444	5
ACAGCGC	145	1.7157872E-6	9.168973	8
>>END_MODULE