FastQCFastQC Report
Mon 15 Aug 2016
SRR2921157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526577
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57890.2291242261763643No Hit
GGTATCAACGCAGAGTACTTTTTTT40920.16195825419134266No Hit
TATCAACGCAGAGTACTTTTTTTTT36710.14529539372835262No Hit
GGTATCAACGCAGAGTACATGGGGG27950.11062397860821183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26330.10421214156544606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC602.5685289E-514.2505810
GGTATCA17900.013.6081671
TATACCG852.7038368E-713.4107175
GGGACGC2500.011.391896
TCTATAC1604.5474735E-1111.2861793
CCCCTAT1201.2486817E-711.1009331
AAGACCG1900.010.999145
CCTATAC1408.301868E-910.8618873
AGAACCG1553.6707206E-910.4185415
TTGCGCG750.002662483410.12692618
CTAAGAC2450.010.0860383
TAGCACT2900.09.8318814
AGGGACG3000.09.81595
TACACCG1554.0710802E-89.8056855
GTCCTAC2002.564775E-109.5150851
TGCCGGT2401.8189894E-129.50848419
TAAGACA3200.09.504154
CCGCTCG1201.7007535E-59.5001989
CCTCTAG3250.09.3686991
GACGCTC2650.09.3183658
Produced by FastQC (version 0.11.5)