##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2526577
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.603904808759044	31.0	30.0	33.0	26.0	34.0
2	29.862398414930556	31.0	30.0	34.0	26.0	34.0
3	29.968561021492715	31.0	30.0	34.0	26.0	34.0
4	33.65184674759566	35.0	35.0	37.0	30.0	37.0
5	33.16373773686691	35.0	35.0	37.0	27.0	37.0
6	33.09771877128621	36.0	35.0	37.0	26.0	37.0
7	32.96342244863307	36.0	35.0	37.0	26.0	37.0
8	32.89796471668981	37.0	35.0	37.0	26.0	37.0
9	34.29758760568152	39.0	35.0	39.0	26.0	39.0
10	34.16355844290516	38.0	35.0	39.0	25.0	39.0
11	34.17553828757247	38.0	35.0	39.0	25.0	39.0
12	34.0475473338038	38.0	35.0	39.0	25.0	39.0
13	34.02339608094272	38.0	35.0	39.0	25.0	39.0
14	34.982241586145996	39.0	35.0	41.0	25.0	41.0
15	34.966912546104865	39.0	35.0	41.0	25.0	41.0
16	34.95182375205663	39.0	35.0	41.0	24.0	41.0
17	34.926088933762955	39.0	35.0	41.0	24.0	41.0
18	34.88081344839283	39.0	35.0	41.0	24.0	41.0
19	34.91743809905655	39.0	35.0	41.0	24.0	41.0
20	34.85374599705451	39.0	35.0	41.0	23.0	41.0
21	34.77978308201175	39.0	35.0	41.0	22.0	41.0
22	34.69285836133235	39.0	35.0	41.0	21.0	41.0
23	34.565073615409304	39.0	34.0	41.0	18.0	41.0
24	34.48931380282493	39.0	34.0	41.0	18.0	41.0
25	34.35320514672618	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83623.0
3	2.0
4	1372.0
5	11887.0
6	13399.0
7	9967.0
8	7727.0
9	6749.0
10	5953.0
11	5456.0
12	5056.0
13	4774.0
14	4668.0
15	4656.0
16	4777.0
17	4827.0
18	5475.0
19	6485.0
20	8107.0
21	10306.0
22	12965.0
23	15787.0
24	18736.0
25	21396.0
26	24286.0
27	27351.0
28	31167.0
29	37847.0
30	48123.0
31	61065.0
32	77672.0
33	100815.0
34	133023.0
35	177667.0
36	241691.0
37	367071.0
38	924649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40515334195446	18.87781137481617	9.333954561590344	31.38308072163903
2	14.972431079678158	20.8716377929507	34.99473793990842	29.161193187462718
3	17.88594612593843	23.732920604791435	28.296691005138914	30.084442264131223
4	13.370682796608055	16.310458074125233	33.24764895992688	37.07121016933983
5	14.476593617805793	37.64269201811852	32.77020243325461	15.11051193082108
6	33.97030895125035	35.0677124687997	16.350184332125565	14.611794247824388
7	29.547289595339116	32.25931701602178	19.53751345703249	18.655879931606613
8	27.808563374305447	34.940987969604585	18.52694351010159	18.72350514598838
9	27.128324210977933	15.58092233088483	18.78323914133628	38.50751431680095
10	16.632226130452388	28.75115225065375	31.24994805224618	23.36667356664768
11	35.94452881913563	22.565803353270564	21.62016031323263	19.869507514361178
12	23.32179070734832	25.09806746439946	28.64052827204554	22.939613556206677
13	28.75291457865216	21.14795709360255	25.180178450779984	24.9189498769653
14	22.622651918166977	20.695316964563286	25.715528101341217	30.96650301592852
15	24.56445041258842	28.17390629626873	22.68947317581445	24.5721701153284
16	24.56607955669899	27.26103304967346	24.16113200079161	24.01175539283594
17	23.411160633536994	27.434707115595526	25.62941877488792	23.524713475979556
18	23.404171103098047	26.860154447184364	26.498141646513062	23.237532803204523
19	24.56919686325919	26.23885587806419	26.343548481581223	22.848398777095397
20	24.735996567688222	25.731810271367152	26.170189944735505	23.362003216209125
21	24.81507783633745	25.421958215468464	26.01755681858667	23.74540712960742
22	24.898370162564206	25.148630297743463	25.81241532496019	24.14058421473214
23	24.32733886273276	25.16545649209676	26.078500056402248	24.428704588768234
24	23.948609737281572	25.725490848019287	26.108606222388996	24.217293192310144
25	23.943520695056712	25.383930104203795	26.302349194316704	24.370200006422788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	964.0
1	964.0
2	985.5
3	1007.0
4	1007.0
5	1007.0
6	1446.5
7	1886.0
8	1886.0
9	1886.0
10	2520.5
11	3155.0
12	3155.0
13	3155.0
14	4393.0
15	5631.0
16	5631.0
17	5631.0
18	10833.5
19	16036.0
20	16036.0
21	16036.0
22	26291.5
23	36547.0
24	36547.0
25	36547.0
26	55165.0
27	73783.0
28	73783.0
29	73783.0
30	96416.0
31	119049.0
32	119049.0
33	119049.0
34	146291.5
35	173534.0
36	173534.0
37	173534.0
38	203950.0
39	234366.0
40	234366.0
41	234366.0
42	260700.5
43	287035.0
44	287035.0
45	287035.0
46	312178.0
47	337321.0
48	337321.0
49	337321.0
50	341195.5
51	345070.0
52	345070.0
53	345070.0
54	327157.0
55	309244.0
56	309244.0
57	309244.0
58	273453.0
59	237662.0
60	237662.0
61	237662.0
62	202376.5
63	167091.0
64	167091.0
65	167091.0
66	131475.5
67	95860.0
68	95860.0
69	95860.0
70	71943.0
71	48026.0
72	48026.0
73	48026.0
74	34024.5
75	20023.0
76	20023.0
77	20023.0
78	14310.0
79	8597.0
80	8597.0
81	8597.0
82	5937.0
83	3277.0
84	3277.0
85	3277.0
86	2158.5
87	1040.0
88	1040.0
89	1040.0
90	681.5
91	323.0
92	323.0
93	323.0
94	184.5
95	46.0
96	46.0
97	46.0
98	25.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10072916835703008
2	0.0
3	0.037441961990471694
4	0.056123363744702816
5	0.06514743069378055
6	0.0062930993197515845
7	6.728470970803581E-4
8	0.005659831463675953
9	0.0
10	0.0
11	0.005857727668699589
12	0.0
13	0.002137279014255255
14	0.058735593651014786
15	0.02267890509570854
16	0.003047601557363975
17	0.0
18	0.005818148427694862
19	0.005264039053628684
20	0.0
21	0.015000532340791512
22	2.374754460283617E-4
23	0.0032454977623876096
24	0.007282580344869758
25	0.17050737024836368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2526577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.50556271685595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.893773619604154	25.475476918486162
2	15.42233376366324	12.493806150086929
3	7.458207566670221	9.062966830412705
4	4.238774385781731	6.867757669035377
5	2.6016905866033944	5.269147061275876
6	1.68362756930742	4.091776926024566
7	1.1587792013306213	3.285590253014001
8	0.8398441095703528	2.721468660206717
9	0.6334897721025747	2.309387372494886
>10	2.8920125540337014	19.759966082416046
>50	0.12072578063429405	3.3175624703347144
>100	0.053588693472961975	3.7048804891174276
>500	0.00226578550568639	0.6571626280364572
>1k	7.880993063251483E-4	0.7520519795128215
>5k	9.851241329064354E-5	0.2309985095452711
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5789	0.2291242261763643	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4092	0.16195825419134266	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3671	0.14529539372835262	No Hit
GGTATCAACGCAGAGTACATGGGGG	2795	0.11062397860821183	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2633	0.10421214156544606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9579241004726946E-5
2	0.0	0.0	0.0	0.0	3.9579241004726946E-5
3	0.0	0.0	0.0	0.0	3.9579241004726946E-5
4	0.0	3.9579241004726946E-5	0.0	0.0	7.915848200945389E-5
5	0.0	3.9579241004726946E-5	0.0	0.0	7.915848200945389E-5
6	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
7	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
8	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
9	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
10	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
11	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
12	0.0	3.9579241004726946E-5	0.0	0.0	1.1873772301418085E-4
13	0.0	3.9579241004726946E-5	0.0	3.9579241004726946E-5	1.1873772301418085E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	60	2.5685289E-5	14.25058	10
GGTATCA	1790	0.0	13.608167	1
TATACCG	85	2.7038368E-7	13.410717	5
GGGACGC	250	0.0	11.39189	6
TCTATAC	160	4.5474735E-11	11.286179	3
CCCCTAT	120	1.2486817E-7	11.100933	1
AAGACCG	190	0.0	10.99914	5
CCTATAC	140	8.301868E-9	10.861887	3
AGAACCG	155	3.6707206E-9	10.418541	5
TTGCGCG	75	0.0026624834	10.126926	18
CTAAGAC	245	0.0	10.086038	3
TAGCACT	290	0.0	9.831881	4
AGGGACG	300	0.0	9.8159	5
TACACCG	155	4.0710802E-8	9.805685	5
GTCCTAC	200	2.564775E-10	9.515085	1
TGCCGGT	240	1.8189894E-12	9.508484	19
TAAGACA	320	0.0	9.50415	4
CCGCTCG	120	1.7007535E-5	9.500198	9
CCTCTAG	325	0.0	9.368699	1
GACGCTC	265	0.0	9.318365	8
>>END_MODULE