FastQCFastQC Report
Mon 15 Aug 2016
SRR2921156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500828
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30000.19988966090717925No Hit
TATCAACGCAGAGTACTTTTTTTTT18070.12040020575309095No Hit
GGTATCAACGCAGAGTACTTTTTTT16840.11220472965589662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.012.5809341
AGACCGC658.0516376E-411.68736656
CAACTCA2950.011.5883216
TGTCCGA600.00588817111.08012610
TCAACTC2950.010.944165
GCTCTAA700.001489146310.8590681
TATAAGG1053.4662935E-610.8568952
TTCGCGC700.001493293410.85544817
GTCCAAA1659.258656E-1010.3654731
AGAGCGA1106.0645125E-610.36063710
ACAGTCC1951.4551915E-1110.2264458
GTATATA1502.4361725E-810.135131
CTAGTAG856.614277E-410.0552433
TAGGCCC856.614277E-410.0552434
GTATCAA21900.09.805391
TATACTG1651.0288204E-89.7834165
CCCTAGA1752.562956E-99.7731611
AACTCAG3950.09.6161877
GTTCTAA900.00111166249.5016841
GTATTAT900.00111166249.5016841
Produced by FastQC (version 0.11.5)