##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921156_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1500828
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.570480428137003	30.0	26.0	31.0	2.0	34.0
2	25.160490076144633	30.0	25.0	31.0	2.0	34.0
3	25.055705250701614	30.0	24.0	31.0	2.0	34.0
4	28.666388820038005	35.0	28.0	37.0	2.0	37.0
5	28.43188360025266	35.0	27.0	37.0	2.0	37.0
6	28.541484433925806	35.0	28.0	37.0	2.0	37.0
7	28.466228641789733	35.0	28.0	37.0	2.0	37.0
8	28.178666709309795	35.0	27.0	37.0	2.0	37.0
9	29.699465228527185	35.0	28.0	39.0	2.0	39.0
10	29.08222327941643	35.0	27.0	39.0	2.0	39.0
11	29.48980829248921	35.0	27.0	39.0	2.0	39.0
12	29.193545163069984	35.0	27.0	39.0	2.0	39.0
13	29.166210251940928	35.0	27.0	39.0	2.0	39.0
14	30.156113825168507	37.0	27.0	40.0	2.0	41.0
15	29.81351027566117	37.0	27.0	40.0	2.0	41.0
16	29.72591995884938	37.0	27.0	40.0	2.0	41.0
17	30.017209167206367	37.0	27.0	40.0	2.0	41.0
18	30.053934894604843	37.0	27.0	40.0	2.0	41.0
19	29.753600012792937	37.0	27.0	40.0	2.0	41.0
20	29.6234205385294	37.0	27.0	40.0	2.0	41.0
21	29.549000285175918	37.0	27.0	40.0	2.0	41.0
22	28.81735948423137	37.0	22.0	40.0	2.0	41.0
23	28.87729373385891	37.0	24.0	40.0	2.0	41.0
24	28.518787629228665	36.0	19.0	40.0	2.0	41.0
25	28.032229542625803	36.0	17.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	191657.0
3	15.0
4	1531.0
5	7981.0
6	9101.0
7	7878.0
8	6529.0
9	6006.0
10	5714.0
11	5353.0
12	5169.0
13	5036.0
14	4934.0
15	4953.0
16	5193.0
17	5653.0
18	6700.0
19	8153.0
20	10229.0
21	12684.0
22	14865.0
23	17369.0
24	19492.0
25	21520.0
26	23872.0
27	26246.0
28	29359.0
29	33750.0
30	39134.0
31	46227.0
32	57203.0
33	71636.0
34	90534.0
35	114659.0
36	148382.0
37	190169.0
38	245942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38419864800554	18.37450060054576	12.523411953060117	25.71788879838859
2	16.283340999846672	19.925934814637042	37.8709092484817	25.91981493703459
3	18.93681973542777	23.3296303972182	30.27333532807636	27.460214539277665
4	13.580325067919619	16.015702309167033	33.96334039906149	36.440632223851864
5	13.990877499275332	36.415103769278694	34.28920792837937	15.304810803066598
6	33.59528042153181	33.890254075793635	16.846280546797335	15.668184955877217
7	28.831210195326918	31.915968245549276	20.581622984529233	18.671198574594573
8	26.951369676810643	33.72426025725084	20.368112303370488	18.95625776256803
9	27.03028493789286	15.904106914513303	19.151696560235035	37.913911587358804
10	16.353771105041623	28.24635669627597	32.42288776339032	22.976984435292085
11	35.227342394332034	22.82460063812603	21.828494966161195	20.119562001380743
12	23.45367058950529	25.10936570224942	28.787385078553395	22.649578629691895
13	28.453608384814416	21.779180962649086	24.92681811912071	24.840392533415784
14	22.868469928567478	21.53591585103597	25.472201693082063	30.123412527314493
15	24.523611047252746	28.176998061593224	22.950896138312554	24.348494752841475
16	24.087928875215034	27.423619437877456	24.603875498300727	23.884576188606786
17	23.11774308478763	27.498005275272014	25.722450301858345	23.66180133808201
18	23.186802420862442	26.97711430584149	26.468202687581183	23.367880585714886
19	24.424398608253846	26.37973397949167	26.758725569970167	22.43714184228432
20	24.665597752347747	25.792555984720657	26.243512543083114	23.298333719848486
21	25.07597690302148	25.461259777027784	25.970771552114826	23.491991767835913
22	24.42449866232213	24.8353640049709	27.06473827824366	23.675399054463313
23	24.527092273069268	25.276747234526916	26.270244982363216	23.925915510040603
24	24.292296291952436	25.584155433591803	26.21562439192125	23.90792388253451
25	23.769674793972364	25.43168571472561	26.795005934318837	24.003633556983182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1415.0
1	1415.0
2	1473.5
3	1532.0
4	1532.0
5	1532.0
6	2040.0
7	2548.0
8	2548.0
9	2548.0
10	3160.5
11	3773.0
12	3773.0
13	3773.0
14	4931.0
15	6089.0
16	6089.0
17	6089.0
18	9822.5
19	13556.0
20	13556.0
21	13556.0
22	19644.0
23	25732.0
24	25732.0
25	25732.0
26	36217.0
27	46702.0
28	46702.0
29	46702.0
30	60159.0
31	73616.0
32	73616.0
33	73616.0
34	88043.5
35	102471.0
36	102471.0
37	102471.0
38	119699.0
39	136927.0
40	136927.0
41	136927.0
42	153227.0
43	169527.0
44	169527.0
45	169527.0
46	183594.0
47	197661.0
48	197661.0
49	197661.0
50	201130.5
51	204600.0
52	204600.0
53	204600.0
54	193278.5
55	181957.0
56	181957.0
57	181957.0
58	160532.5
59	139108.0
60	139108.0
61	139108.0
62	117228.0
63	95348.0
64	95348.0
65	95348.0
66	74957.0
67	54566.0
68	54566.0
69	54566.0
70	40221.5
71	25877.0
72	25877.0
73	25877.0
74	18280.0
75	10683.0
76	10683.0
77	10683.0
78	7616.0
79	4549.0
80	4549.0
81	4549.0
82	3122.5
83	1696.0
84	1696.0
85	1696.0
86	1177.0
87	658.0
88	658.0
89	658.0
90	421.0
91	184.0
92	184.0
93	184.0
94	113.0
95	42.0
96	42.0
97	42.0
98	26.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03504732054572542
2	0.05317064980130967
3	0.014458685472285964
4	0.01046089225414238
5	0.009528073836575545
6	0.001066078191504956
7	0.004131052992081704
8	0.0023986759308861506
9	0.007662437001441871
10	0.0040644231051126445
11	0.012259899202306994
12	0.008595255419008708
13	0.007662437001441871
14	0.016724101629233996
15	0.007395917453565631
16	0.031915715858179615
17	0.0413771598077861
18	0.024719688065521165
19	0.017790179820738952
20	0.01599117287257434
21	0.024786317952490224
22	0.006196579488122556
23	0.018056699368615193
24	0.013325977393811951
25	0.46474346160919167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1500828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.25714857381833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68752316425594	43.909089075739374
2	10.90784012897271	12.491036457680964
3	4.642989328658944	7.975329894530344
4	2.5645325426331573	5.873512832637552
5	1.5068501160192989	4.313897048569619
6	0.9346855329836352	3.211045705910555
7	0.6423126885339302	2.574389512676516
8	0.46114474378036685	2.1123066486934285
9	0.3212562412314817	1.6554794701011633
>10	1.2774946470508635	11.900675527087502
>50	0.03612673743141157	1.4251637258488956
>100	0.015328182772472696	1.4906091031550512
>500	0.0014369592568100023	0.5424743438361017
>1k	4.7898641893666746E-4	0.5249906535329917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3000	0.19988966090717925	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1807	0.12040020575309095	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1684	0.11220472965589662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.662988696905975E-5	0.0
10	0.0	0.0	0.0	6.662988696905975E-5	0.0
11	0.0	0.0	0.0	6.662988696905975E-5	0.0
12	0.0	0.0	0.0	6.662988696905975E-5	0.0
13	0.0	0.0	0.0	6.662988696905975E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1080	0.0	12.580934	1
AGACCGC	65	8.0516376E-4	11.6873665	6
CAACTCA	295	0.0	11.588321	6
TGTCCGA	60	0.005888171	11.080126	10
TCAACTC	295	0.0	10.94416	5
GCTCTAA	70	0.0014891463	10.859068	1
TATAAGG	105	3.4662935E-6	10.856895	2
TTCGCGC	70	0.0014932934	10.855448	17
GTCCAAA	165	9.258656E-10	10.365473	1
AGAGCGA	110	6.0645125E-6	10.360637	10
ACAGTCC	195	1.4551915E-11	10.226445	8
GTATATA	150	2.4361725E-8	10.13513	1
CTAGTAG	85	6.614277E-4	10.055243	3
TAGGCCC	85	6.614277E-4	10.055243	4
GTATCAA	2190	0.0	9.80539	1
TATACTG	165	1.0288204E-8	9.783416	5
CCCTAGA	175	2.562956E-9	9.773161	1
AACTCAG	395	0.0	9.616187	7
GTTCTAA	90	0.0011116624	9.501684	1
GTATTAT	90	0.0011116624	9.501684	1
>>END_MODULE