FastQCFastQC Report
Mon 15 Aug 2016
SRR2921156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500828
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49490.32975131060987667No Hit
TATCAACGCAGAGTACTTTTTTTTT34320.22867377207781303No Hit
GGTATCAACGCAGAGTACTTTTTTT34320.22867377207781303No Hit
CTTATACACATCTCCGAGCCCACGA24960.1663081978747731No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24400.16257692420450578No Hit
GGTATCAACGCAGAGTACATGGGGG19030.1267966749021207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGT400.005295420514.24332615
CTTATAC3800.013.5198111
CCCTACG604.0415692E-412.6852541
GGTATCA14350.012.5968551
CCTACAC2150.012.37867453
CATGTCG701.0915482E-412.213455
TATACAC4350.012.0178843
TAGACCT1157.019662E-811.5713694
TCTATAC752.0635036E-411.4060653
CATTCCG752.0737563E-411.399989
TTATACA4550.011.2807232
TTTAGGC1104.933099E-711.2332463
ACTGTTC1104.975809E-711.2257578
CTAGACA1303.2390744E-810.967374
GTAATAG700.001484746510.8629193
ACCGTGC700.001493031210.8556778
GTCCTAG1855.456968E-1210.7996071
TTTAAGC909.462481E-510.5611723
TCGCGCA909.575096E-510.54885518
TACACAT5750.010.4079845
Produced by FastQC (version 0.11.5)