##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1500828
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.028197101866436	31.0	30.0	33.0	25.0	34.0
2	29.30771214289712	31.0	30.0	34.0	25.0	34.0
3	29.4280070734288	31.0	30.0	34.0	25.0	34.0
4	33.0786779031308	35.0	33.0	37.0	28.0	37.0
5	32.5145639606937	35.0	33.0	37.0	25.0	37.0
6	32.42912778812762	35.0	33.0	37.0	23.0	37.0
7	32.27231834693916	35.0	33.0	37.0	22.0	37.0
8	32.19298147422623	35.0	33.0	37.0	19.0	37.0
9	33.51925936882841	38.0	34.0	39.0	18.0	39.0
10	33.368170103436235	38.0	33.0	39.0	17.0	39.0
11	33.37010570165269	38.0	33.0	39.0	17.0	39.0
12	33.23718707273585	38.0	33.0	39.0	17.0	39.0
13	33.20920385280658	38.0	33.0	39.0	17.0	39.0
14	34.11709203186508	39.0	33.0	41.0	17.0	41.0
15	34.096985797173296	39.0	33.0	41.0	17.0	41.0
16	34.07129997574672	39.0	33.0	41.0	17.0	41.0
17	34.04897096802565	39.0	33.0	41.0	16.0	41.0
18	33.99367482483003	39.0	33.0	41.0	13.0	41.0
19	34.03326030697721	39.0	34.0	41.0	11.0	41.0
20	33.96024394534217	39.0	34.0	41.0	10.0	41.0
21	33.88670120760007	39.0	34.0	41.0	10.0	41.0
22	33.78991196859334	39.0	34.0	41.0	2.0	41.0
23	33.64860796840144	39.0	34.0	41.0	2.0	41.0
24	33.56477690981245	39.0	34.0	41.0	2.0	41.0
25	33.42197240456601	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66402.0
3	3.0
4	1139.0
5	9042.0
6	10002.0
7	7546.0
8	5980.0
9	5028.0
10	4347.0
11	3986.0
12	3690.0
13	3361.0
14	3208.0
15	3231.0
16	3342.0
17	3471.0
18	3775.0
19	4437.0
20	5413.0
21	7163.0
22	8773.0
23	10690.0
24	12354.0
25	13793.0
26	15510.0
27	17348.0
28	19617.0
29	23946.0
30	29701.0
31	37633.0
32	47040.0
33	60613.0
34	79045.0
35	104679.0
36	140719.0
37	212519.0
38	512282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.58489518997068	19.277601841945888	9.422230878205863	31.715272089877566
2	14.965472392572634	21.08562740034168	34.735159525275385	29.213740681810307
3	17.74554888181299	23.768447869375652	28.08615415789863	30.399849090912728
4	13.257067429325705	16.662566707666258	32.36360969723636	37.71675616577168
5	14.657149715291576	37.63598661136967	32.258731280587824	15.448132392750939
6	34.23983879608824	34.73373900852131	16.02337030255053	15.003051892839913
7	29.514509155383323	32.431065603863516	19.029511377515224	19.024913863237945
8	27.627938589477054	34.72233328002559	18.44461325230556	19.2051148781918
9	27.1653380667205	15.695136284770806	18.777701375507387	38.361824273001304
10	16.984491227509082	28.941357703880794	30.583984307329022	23.490166761281106
11	35.413916562992895	22.586977685761518	21.64701696074035	20.35208879050524
12	23.53234347973252	25.189428768653038	28.11621318365595	23.162014567958487
13	28.742271585755	21.40614994872711	24.928287911790633	24.923290553727263
14	22.909136972921438	21.203017544561455	25.282303094549953	30.605542387967155
15	24.669602643445437	28.071945648885077	22.414903103435865	24.843548604233618
16	24.52782036750884	27.300361885000395	23.971832806597753	24.199984940893003
17	23.01397829330096	27.486179286486717	25.705760890495704	23.794081529716614
18	23.256351685608887	26.66835032091605	26.539210930336694	23.536087063138368
19	24.55716580786297	26.089685626538	26.415593467450194	22.937555098148835
20	24.529875861774876	25.661851765726496	26.140454562717757	23.667817809780875
21	24.850088700573256	25.180766570213404	26.10162646595857	23.867518263254773
22	24.723584808858078	25.222378136781042	25.8610281565702	24.193008897790676
23	24.479130573842586	25.07382925984168	26.083117886937284	24.363922279378446
24	24.32553459454777	25.548521660325058	26.002385601098176	24.123558144029
25	23.913828539540198	25.589970649156292	26.205690872935612	24.2905099383679
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1184.0
1	1184.0
2	1287.5
3	1391.0
4	1391.0
5	1391.0
6	1637.0
7	1883.0
8	1883.0
9	1883.0
10	2360.0
11	2837.0
12	2837.0
13	2837.0
14	3484.0
15	4131.0
16	4131.0
17	4131.0
18	7604.5
19	11078.0
20	11078.0
21	11078.0
22	17150.0
23	23222.0
24	23222.0
25	23222.0
26	33416.0
27	43610.0
28	43610.0
29	43610.0
30	55720.5
31	67831.0
32	67831.0
33	67831.0
34	81990.0
35	96149.0
36	96149.0
37	96149.0
38	112843.5
39	129538.0
40	129538.0
41	129538.0
42	145857.0
43	162176.0
44	162176.0
45	162176.0
46	179397.5
47	196619.0
48	196619.0
49	196619.0
50	204513.5
51	212408.0
52	212408.0
53	212408.0
54	201388.5
55	190369.0
56	190369.0
57	190369.0
58	168895.0
59	147421.0
60	147421.0
61	147421.0
62	125284.5
63	103148.0
64	103148.0
65	103148.0
66	81581.5
67	60015.0
68	60015.0
69	60015.0
70	43676.5
71	27338.0
72	27338.0
73	27338.0
74	19202.0
75	11066.0
76	11066.0
77	11066.0
78	7857.0
79	4648.0
80	4648.0
81	4648.0
82	3240.0
83	1832.0
84	1832.0
85	1832.0
86	1263.5
87	695.0
88	695.0
89	695.0
90	452.0
91	209.0
92	209.0
93	209.0
94	118.5
95	28.0
96	28.0
97	28.0
98	15.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10287654548022825
2	0.0
3	0.039111743650838075
4	0.05417009810584557
5	0.06982812154357461
6	0.00799558643628717
7	0.0011327080784740155
8	0.007196027792658452
9	0.0
10	0.0
11	0.008461995645070587
12	0.0
13	0.0023320460439170913
14	0.06216568454213274
15	0.024120019082799628
16	0.004997241522679481
17	6.662988696905975E-5
18	0.008262105984163408
19	0.006463099035998796
20	6.662988696905975E-5
21	0.01825658902952237
22	3.997793218143585E-4
23	0.004530832313896062
24	0.01052752214111144
25	0.182899039730069
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1500828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.13538554384418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.30812607330368	26.453395596274852
2	14.188792387102694	11.673228904900611
3	7.120389444843468	8.78699895107864
4	4.172130000692213	6.864887044700523
5	2.6040571386829767	5.355944718896199
6	1.7627001502082258	4.350561016620445
7	1.2261395898679972	3.530640733185373
8	0.9018793545935697	2.9679323972191876
9	0.6593059680610117	2.4408724668792416
>10	2.917929792609879	19.8818986706592
>50	0.09766479412474456	2.710209231728515
>100	0.03630621293212179	2.6319903605295814
>500	0.0029437026282950196	0.8109689329643838
>1k	0.0016353903490527886	1.5404709743634528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4949	0.32975131060987667	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3432	0.22867377207781303	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3432	0.22867377207781303	No Hit
CTTATACACATCTCCGAGCCCACGA	2496	0.1663081978747731	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2440	0.16257692420450578	No Hit
GGTATCAACGCAGAGTACATGGGGG	1903	0.1267966749021207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.662988696905975E-5	0.0
12	0.0	0.0	0.0	6.662988696905975E-5	0.0
13	0.0	0.0	0.0	6.662988696905975E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGT	40	0.0052954205	14.243326	15
CTTATAC	380	0.0	13.519811	1
CCCTACG	60	4.0415692E-4	12.685254	1
GGTATCA	1435	0.0	12.596855	1
CCTACAC	215	0.0	12.3786745	3
CATGTCG	70	1.0915482E-4	12.21345	5
TATACAC	435	0.0	12.017884	3
TAGACCT	115	7.019662E-8	11.571369	4
TCTATAC	75	2.0635036E-4	11.406065	3
CATTCCG	75	2.0737563E-4	11.39998	9
TTATACA	455	0.0	11.280723	2
TTTAGGC	110	4.933099E-7	11.233246	3
ACTGTTC	110	4.975809E-7	11.225757	8
CTAGACA	130	3.2390744E-8	10.96737	4
GTAATAG	70	0.0014847465	10.862919	3
ACCGTGC	70	0.0014930312	10.855677	8
GTCCTAG	185	5.456968E-12	10.799607	1
TTTAAGC	90	9.462481E-5	10.561172	3
TCGCGCA	90	9.575096E-5	10.548855	18
TACACAT	575	0.0	10.407984	5
>>END_MODULE