FastQCFastQC Report
Mon 15 Aug 2016
SRR2921155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039333
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30380.1489702760657529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC751.4746462E-512.6703981
TAGTACG1001.4428042E-712.3503032
GAACCGT701.0954784E-412.2091926
GGTATCA11300.011.6892821
GTATCAA24100.010.804011
TAATACC1502.1500455E-910.7619144
GTATAAG2750.010.7122441
TCTAGGA3200.010.6877622
ACACCGT1451.4531906E-810.4783886
GGTTTAC856.5769925E-410.0617861
ACCGTCC1151.0314414E-59.9091548
ACCGTGC1252.5833942E-69.8761238
GTGTTAC2850.09.6695141
CTCTATA1901.0513759E-99.5002342
GTCCGCC1002.76054E-49.49836912
CGTCCCA1002.7639422E-49.49720510
CGCGTCC1106.8613845E-59.49697118
TATACTG2303.6379788E-129.4962725
TGCACCG1106.866909E-59.4962725
ATGTCGT800.004532039.4960396
Produced by FastQC (version 0.11.5)