##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921155_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2039333
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95621999938215	30.0	26.0	31.0	2.0	34.0
2	25.755799567799865	31.0	26.0	31.0	2.0	34.0
3	25.371204702714074	30.0	25.0	31.0	2.0	34.0
4	29.087037771663578	35.0	28.0	37.0	2.0	37.0
5	28.94770103754512	35.0	28.0	37.0	2.0	37.0
6	29.123683086577817	35.0	29.0	37.0	2.0	37.0
7	29.084095142872695	35.0	29.0	37.0	2.0	37.0
8	28.72413038969114	35.0	28.0	37.0	2.0	37.0
9	30.20733445690331	37.0	29.0	39.0	2.0	39.0
10	29.616937989038572	35.0	27.0	39.0	2.0	39.0
11	30.05600213403108	37.0	29.0	39.0	2.0	39.0
12	29.77077603314417	37.0	27.0	39.0	2.0	39.0
13	29.759009931188285	37.0	27.0	39.0	2.0	39.0
14	30.75701761311174	37.0	29.0	40.0	2.0	41.0
15	30.43169359785773	37.0	27.0	40.0	2.0	41.0
16	30.34651231554631	37.0	27.0	40.0	2.0	41.0
17	30.60903589555997	37.0	29.0	40.0	2.0	41.0
18	30.63946545267497	37.0	29.0	40.0	2.0	41.0
19	30.367254881865787	37.0	27.0	40.0	2.0	41.0
20	30.254305206653353	37.0	27.0	40.0	2.0	41.0
21	30.1846093796354	37.0	27.0	40.0	2.0	41.0
22	29.45977581885842	37.0	25.0	40.0	2.0	41.0
23	29.52623382252923	37.0	27.0	40.0	2.0	41.0
24	29.1750690054052	37.0	25.0	40.0	2.0	41.0
25	28.69208265643718	37.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	238547.0
3	13.0
4	1708.0
5	9899.0
6	11714.0
7	10212.0
8	8643.0
9	7780.0
10	7179.0
11	6854.0
12	6647.0
13	6166.0
14	6114.0
15	6376.0
16	6743.0
17	7176.0
18	8443.0
19	10221.0
20	12766.0
21	16052.0
22	19117.0
23	22056.0
24	25202.0
25	28460.0
26	31248.0
27	34823.0
28	38854.0
29	44873.0
30	52790.0
31	62818.0
32	76642.0
33	96455.0
34	122738.0
35	157929.0
36	206025.0
37	268907.0
38	361143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81458039939351	18.188096874339326	12.56184880025468	25.435473926012474
2	15.307100150173502	19.190711516261764	40.19886091853591	25.303327415028832
3	19.135626885069026	23.956008925725214	29.214192883941053	27.69417130526471
4	13.520857659463761	15.46428356000669	35.2711522702563	35.74370651027325
5	13.58301494195886	36.991119369425704	34.94429315643671	14.481572532178738
6	33.70880236314529	35.619099737362916	16.567662883045102	14.10443501644669
7	29.398178455997986	31.68734329479482	19.784588421578622	19.129889827628567
8	27.132099971362777	34.62112563894348	19.785475829008327	18.46129856068541
9	26.885811214386806	16.188832690434786	18.653767503170357	38.27158859200805
10	15.699944391647566	28.52458389358503	32.716394460348894	23.05907725441851
11	36.079101021325926	22.895581120035466	21.55651915488786	19.468798703750746
12	23.458909147974147	24.775101268181686	29.20564551722785	22.56034406661632
13	28.52210151341803	21.51563463172808	25.085537714322427	24.876726140531463
14	22.37335765886386	20.843301406074517	25.788936787951016	30.99440414711061
15	24.281555788108854	28.310198421144	23.11565625309558	24.29258953765157
16	24.204198135350207	27.55624140676236	24.643897213228055	23.595663244659377
17	23.41996474829567	27.42392259341873	25.6255264331368	23.5305862251488
18	23.32162409519895	27.232905280732332	26.337300610247173	23.108170013821542
19	24.366525368657346	26.482758553041684	26.574325156097274	22.5763909222037
20	24.44424390834825	26.019395001125563	26.422439858380102	23.11392123214609
21	25.0185400268389	25.78182017939683	25.943039936591145	23.25659985717313
22	24.069089398704303	25.15597929201118	27.169010789946906	23.605920519337605
23	24.309169807101142	25.520452222564437	26.28877153265627	23.88160643767815
24	23.841120661160268	25.850750484776974	26.441996937837715	23.866131916225047
25	23.7365115821927	25.574774226191092	26.959296914913168	23.729417276703042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1093.0
1	1093.0
2	1237.5
3	1382.0
4	1382.0
5	1382.0
6	1867.5
7	2353.0
8	2353.0
9	2353.0
10	3150.5
11	3948.0
12	3948.0
13	3948.0
14	5931.0
15	7914.0
16	7914.0
17	7914.0
18	13007.5
19	18101.0
20	18101.0
21	18101.0
22	27392.0
23	36683.0
24	36683.0
25	36683.0
26	52625.5
27	68568.0
28	68568.0
29	68568.0
30	86823.5
31	105079.0
32	105079.0
33	105079.0
34	125773.0
35	146467.0
36	146467.0
37	146467.0
38	169720.5
39	192974.0
40	192974.0
41	192974.0
42	214603.0
43	236232.0
44	236232.0
45	236232.0
46	251145.5
47	266059.0
48	266059.0
49	266059.0
50	269144.0
51	272229.0
52	272229.0
53	272229.0
54	255738.5
55	239248.0
56	239248.0
57	239248.0
58	212010.0
59	184772.0
60	184772.0
61	184772.0
62	155143.0
63	125514.0
64	125514.0
65	125514.0
66	98976.5
67	72439.0
68	72439.0
69	72439.0
70	54012.0
71	35585.0
72	35585.0
73	35585.0
74	24935.5
75	14286.0
76	14286.0
77	14286.0
78	9911.0
79	5536.0
80	5536.0
81	5536.0
82	3776.5
83	2017.0
84	2017.0
85	2017.0
86	1329.5
87	642.0
88	642.0
89	642.0
90	404.0
91	166.0
92	166.0
93	166.0
94	99.5
95	33.0
96	33.0
97	33.0
98	23.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03393266327765009
2	0.0502124959484302
3	0.013877086282622797
4	0.009905199396077051
5	0.00863027274113644
6	8.336058897688608E-4
7	0.003628637402523276
8	0.0014220335766645272
9	0.006423668915277692
10	0.0034815304807993596
11	0.010738805285845912
12	0.0094638786309053
13	0.008532201459987163
14	0.015838511905608352
15	0.0066198114775762465
16	0.03265773662270949
17	0.041680294488443036
18	0.02388035695984913
19	0.018633543418362767
20	0.017358616763422156
21	0.02456685592789407
22	0.005884276868956664
23	0.016525010873653295
24	0.012307945784234355
25	0.4676038685197562
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2039333.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.66546417408751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3539320852134	46.15358263555099
2	11.41243648692789	13.618566406996905
3	4.59004562021592	8.21601607531261
4	2.2524041174853737	5.3756294870956305
5	1.303455168139487	3.8885628818577893
6	0.8168577844996918	2.9242919325834515
7	0.5290194933137797	2.2094935537995077
8	0.36186845889488495	1.7272839655940013
9	0.24786464834976313	1.3310063366522036
>10	1.0743407931843656	10.624712039968193
>50	0.040093211151235195	1.6577734666449888
>100	0.0164201832333234	1.5484440995742372
>500	9.254295533365097E-4	0.35207846690520794
>1k	3.3651983757689545E-4	0.37255865146426387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3038	0.1489702760657529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.9035640574638866E-5	0.0
5	0.0	0.0	0.0	4.9035640574638866E-5	0.0
6	0.0	0.0	0.0	4.9035640574638866E-5	1.471069217239166E-4
7	0.0	0.0	0.0	4.9035640574638866E-5	1.471069217239166E-4
8	0.0	0.0	0.0	4.9035640574638866E-5	1.471069217239166E-4
9	0.0	0.0	0.0	4.9035640574638866E-5	1.471069217239166E-4
10	0.0	0.0	0.0	9.807128114927773E-5	1.471069217239166E-4
11	0.0	0.0	0.0	9.807128114927773E-5	1.471069217239166E-4
12	0.0	0.0	0.0	1.471069217239166E-4	3.4324948402247207E-4
13	0.0	0.0	0.0	1.471069217239166E-4	4.413207651717498E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	75	1.4746462E-5	12.670398	1
TAGTACG	100	1.4428042E-7	12.350303	2
GAACCGT	70	1.0954784E-4	12.209192	6
GGTATCA	1130	0.0	11.689282	1
GTATCAA	2410	0.0	10.80401	1
TAATACC	150	2.1500455E-9	10.761914	4
GTATAAG	275	0.0	10.712244	1
TCTAGGA	320	0.0	10.687762	2
ACACCGT	145	1.4531906E-8	10.478388	6
GGTTTAC	85	6.5769925E-4	10.061786	1
ACCGTCC	115	1.0314414E-5	9.909154	8
ACCGTGC	125	2.5833942E-6	9.876123	8
GTGTTAC	285	0.0	9.669514	1
CTCTATA	190	1.0513759E-9	9.500234	2
GTCCGCC	100	2.76054E-4	9.498369	12
CGTCCCA	100	2.7639422E-4	9.497205	10
CGCGTCC	110	6.8613845E-5	9.496971	18
TATACTG	230	3.6379788E-12	9.496272	5
TGCACCG	110	6.866909E-5	9.496272	5
ATGTCGT	80	0.00453203	9.496039	6
>>END_MODULE