FastQCFastQC Report
Mon 15 Aug 2016
SRR2921155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039333
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59000.2893102793903693No Hit
TATCAACGCAGAGTACTTTTTTTTT38970.19109189131936766No Hit
GGTATCAACGCAGAGTACTTTTTTT38020.18643350546477697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25600.12553123987107548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA500.00149960813.30025710
TACCGAT500.001508503913.2898237
GGTATCA14250.013.2890971
GCCGCGT907.484483E-611.6082038
TGTTCGC600.005875872411.08354910
GTCTAGA2250.010.9963381
TATACCG700.001493339810.8557555
GTATCAA31200.010.79707051
AGGACCG1502.1373125E-910.765295
ACAACGC803.780502E-410.6848238
GGTATAC909.458962E-510.561983
TTATACA4150.010.3074752
CTAGCCA1856.002665E-1110.2762694
GTATTAG1303.7543396E-710.2480661
CGATCAG750.002647751510.1335310
TATAGCG750.002650987110.1320385
CCGTCCC856.5936474E-410.0590189
AACAACG856.641397E-410.05112657
TCTAGTA1803.8926373E-1010.0338812
AACCGCT951.6571445E-49.9923487
Produced by FastQC (version 0.11.5)