FastQCFastQC Report
Mon 15 Aug 2016
SRR2921154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824179
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32450.11490064900277212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.012.8281581
AGGTTCG600.00589679111.0784987
CTTAGAC2750.010.7055573
TACGGGT803.78611E-410.6834064
AGCACCG1553.6907295E-910.4153635
GTATCAA34400.010.3322161
CTATTAG1201.5178393E-610.2953821
CTAAGAC2550.010.0555053
GTATAGA3050.09.9708111
GACGCTC1356.486389E-79.8475558
GTTCTAG3200.09.8004121
GTCTTAG3150.09.6542781
TAGACAG3450.09.6339895
ACGTAAC1106.813049E-59.5034291
TCTATAC2106.730261E-119.4968653
TCGACAG900.0011180179.4963615
GACCGTG2201.6370905E-119.4958567
GTCCTAG3250.09.3572231
CCGTCCA1951.6971171E-99.2523739
GTACTAA1651.0605618E-79.2154461
Produced by FastQC (version 0.11.5)