##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921154_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2824179 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.478656629059277 31.0 28.0 31.0 15.0 34.0 2 27.05553436945746 31.0 27.0 31.0 10.0 34.0 3 26.932629624397038 31.0 27.0 31.0 10.0 34.0 4 30.815260647430634 35.0 32.0 37.0 12.0 37.0 5 30.481339532657103 35.0 30.0 37.0 10.0 37.0 6 30.775540785481372 35.0 31.0 37.0 11.0 37.0 7 30.80365550483875 35.0 32.0 37.0 10.0 37.0 8 30.496161185250653 35.0 31.0 37.0 10.0 37.0 9 32.1215910889501 37.0 32.0 39.0 10.0 39.0 10 31.496752153457695 37.0 30.0 39.0 8.0 39.0 11 31.941842921429554 37.0 32.0 39.0 8.0 39.0 12 31.647745769655536 37.0 31.0 39.0 7.0 39.0 13 31.64307715622841 37.0 31.0 39.0 2.0 39.0 14 32.75814528753312 38.0 32.0 40.0 2.0 41.0 15 32.39604819666175 38.0 31.0 40.0 2.0 41.0 16 32.32257976565933 38.0 31.0 40.0 2.0 41.0 17 32.64359624513885 38.0 32.0 40.0 2.0 41.0 18 32.69675080793392 38.0 32.0 40.0 2.0 41.0 19 32.39614025881504 38.0 32.0 40.0 2.0 41.0 20 32.2748260644952 38.0 31.0 40.0 2.0 41.0 21 32.22378999348129 38.0 31.0 40.0 2.0 41.0 22 31.452081472172974 38.0 30.0 40.0 2.0 41.0 23 31.547153703784357 38.0 30.0 40.0 2.0 41.0 24 31.1749131340471 38.0 30.0 40.0 2.0 41.0 25 30.672454543426603 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214621.0 3 7.0 4 1645.0 5 9916.0 6 12073.0 7 10647.0 8 9067.0 9 8512.0 10 7836.0 11 7610.0 12 7558.0 13 7389.0 14 7195.0 15 7510.0 16 7911.0 17 8713.0 18 10266.0 19 12340.0 20 15536.0 21 19325.0 22 24027.0 23 28018.0 24 32581.0 25 36742.0 26 41613.0 27 47255.0 28 52652.0 29 61639.0 30 72667.0 31 86733.0 32 109299.0 33 138635.0 34 178756.0 35 231486.0 36 304783.0 37 403964.0 38 587652.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.483128344869435 17.789702132392453 12.456011280947957 25.27115824179016 2 16.037813722353835 20.012760926764965 38.6855536885349 25.263871662346304 3 18.738691774925194 23.295873279175442 30.053238300269964 27.9121966456294 4 13.900510155298837 15.743351443386079 34.65960962460418 35.69652877671091 5 14.239243368889538 36.50016325021619 33.76180908152808 15.498784299366191 6 34.07742637264115 33.7993962085637 16.978465055375874 15.144712363419274 7 29.65005849709214 31.370942383905227 20.421469424292297 18.557529694710333 8 26.338989952640198 34.301739476829106 20.331925817731154 19.02734475279954 9 27.14524838376245 15.777707979151536 18.937626350169637 38.139417286916384 10 16.196182897149832 27.949288954027136 32.57114974474864 23.28337840407439 11 35.60774053910109 22.64397358816893 21.835548602174452 19.91273727055554 12 23.586799171654963 24.674851909244662 29.06243891521928 22.675910003881096 13 28.815457726030115 21.28103797504214 25.00269125625009 24.900813042677658 14 23.03415710027991 20.906615822218193 25.57387696474508 30.48535011275682 15 24.81204951106846 27.83221760507646 22.961032430776747 24.39470045307833 16 24.45950693998756 26.901536757069977 24.687640347558997 23.951315955383468 17 23.583115841615022 27.073751654212252 25.546067154641573 23.797065349531152 18 23.618316831157184 26.640416182792897 26.09227972985943 23.648987256190484 19 24.599458094008046 26.19737603041643 26.355712306068163 22.84745356950736 20 25.16619755020632 25.56233771568851 25.92883642591336 23.342628308191806 21 25.5706897363857 25.331204576355287 25.44418263913213 23.653923048126885 22 24.534848145766468 24.857508293130653 26.751649419410032 23.855994141692847 23 25.002514385115976 25.041540475225872 25.72368253302698 24.232262606631178 24 24.626143419136174 25.354893290323904 25.849390748959678 24.169572541580244 25 24.099946074309315 25.149682919784468 26.58031441950203 24.170056586404183 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1563.0 1 1563.0 2 1590.5 3 1618.0 4 1618.0 5 1618.0 6 1971.5 7 2325.0 8 2325.0 9 2325.0 10 3053.0 11 3781.0 12 3781.0 13 3781.0 14 6038.0 15 8295.0 16 8295.0 17 8295.0 18 14158.5 19 20022.0 20 20022.0 21 20022.0 22 31400.0 23 42778.0 24 42778.0 25 42778.0 26 62925.0 27 83072.0 28 83072.0 29 83072.0 30 109311.0 31 135550.0 32 135550.0 33 135550.0 34 165636.0 35 195722.0 36 195722.0 37 195722.0 38 229023.0 39 262324.0 40 262324.0 41 262324.0 42 292951.0 43 323578.0 44 323578.0 45 323578.0 46 350050.0 47 376522.0 48 376522.0 49 376522.0 50 383431.0 51 390340.0 52 390340.0 53 390340.0 54 366890.0 55 343440.0 56 343440.0 57 343440.0 58 304670.5 59 265901.0 60 265901.0 61 265901.0 62 223163.5 63 180426.0 64 180426.0 65 180426.0 66 142012.0 67 103598.0 68 103598.0 69 103598.0 70 76742.5 71 49887.0 72 49887.0 73 49887.0 74 35478.0 75 21069.0 76 21069.0 77 21069.0 78 14616.5 79 8164.0 80 8164.0 81 8164.0 82 5584.0 83 3004.0 84 3004.0 85 3004.0 86 1957.5 87 911.0 88 911.0 89 911.0 90 583.0 91 255.0 92 255.0 93 255.0 94 140.0 95 25.0 96 25.0 97 25.0 98 17.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035833422739847576 2 0.053112780740880806 3 0.016287919427203448 4 0.011791037324475537 5 0.010374696504718716 6 8.852130123480134E-4 7 0.0037887116928494976 8 0.001912060106671709 9 0.006444350729893537 10 0.0035408520493920535 11 0.010374696504718716 12 0.008321002316071325 13 0.007754465988168597 14 0.014269633759049976 15 0.0058069973610029675 16 0.029424480530447966 17 0.03834742769491594 18 0.021670014542279365 19 0.015650566058312877 20 0.01526107233287975 21 0.022024099747218574 22 0.005488320676557683 23 0.015119438250904069 24 0.010693373189164001 25 0.45687613993305665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2824179.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.734628955638115 #Duplication Level Percentage of deduplicated Percentage of total 1 68.45000418705503 30.620855393197814 2 12.669098031958312 11.334947993245201 3 6.253310809883307 8.392186164732317 4 3.5964707012001815 6.43547129472055 5 2.358007721620409 5.2742300250609295 6 1.5154615714105468 4.067616665614744 7 1.0578518765754086 3.312582783203846 8 0.7764229962611817 2.7786395720295007 9 0.5862935820029685 2.3604863264977305 >10 2.608177892570167 19.25403698015483 >50 0.09464604637446344 2.8653590532005815 >100 0.03277399909994457 2.4585970641945365 >500 6.580373280611827E-4 0.21864373128425169 >1k 8.225466600745372E-4 0.6263469528632917 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3245 0.11490064900277212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 3.540852049392054E-5 13 0.0 0.0 0.0 3.540852049392054E-5 3.540852049392054E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1615 0.0 12.828158 1 AGGTTCG 60 0.005896791 11.078498 7 CTTAGAC 275 0.0 10.705557 3 TACGGGT 80 3.78611E-4 10.683406 4 AGCACCG 155 3.6907295E-9 10.415363 5 GTATCAA 3440 0.0 10.332216 1 CTATTAG 120 1.5178393E-6 10.295382 1 CTAAGAC 255 0.0 10.055505 3 GTATAGA 305 0.0 9.970811 1 GACGCTC 135 6.486389E-7 9.847555 8 GTTCTAG 320 0.0 9.800412 1 GTCTTAG 315 0.0 9.654278 1 TAGACAG 345 0.0 9.633989 5 ACGTAAC 110 6.813049E-5 9.503429 1 TCTATAC 210 6.730261E-11 9.496865 3 TCGACAG 90 0.001118017 9.496361 5 GACCGTG 220 1.6370905E-11 9.495856 7 GTCCTAG 325 0.0 9.357223 1 CCGTCCA 195 1.6971171E-9 9.252373 9 GTACTAA 165 1.0605618E-7 9.215446 1 >>END_MODULE