FastQCFastQC Report
Mon 15 Aug 2016
SRR2921154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824179
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63260.22399430064454132No Hit
TATCAACGCAGAGTACTTTTTTTTT42180.1493531394433568No Hit
GGTATCAACGCAGAGTACTTTTTTT41630.1474056708161912No Hit
GGTATCAACGCAGAGTACATGGGGG34650.12269052351143465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA456.7383406E-414.7854032
GGTATCA19900.013.9151641
TATACCG752.0776564E-411.3984055
GTATCAA43600.010.8908681
CGAATAC700.001485648210.8627443
TGCACCG2002.5465852E-119.9736055
TAATACC1252.5554527E-69.8849234
GTCTTAG3100.09.8228321
GCACCGT1856.5847416E-109.7493476
GGTCGTG900.00111679859.4974938
TCGCACG900.00112096719.49362518
TAGACAG4000.09.2612055
TAAGGTA1554.2327338E-79.198134
CTTAGAC3000.09.1880713
AACCGCT1356.917575E-69.14127
GTTCGCG1252.7773844E-59.1138816
CTCTTAG4100.09.051681
CGAGCGC1054.4926628E-49.04795710
TTGCGCG1054.5245944E-49.04154818
AGACCGT2106.7848305E-109.0407476
Produced by FastQC (version 0.11.5)