##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2824179
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88423077998951	31.0	30.0	33.0	26.0	34.0
2	30.13168216320566	31.0	30.0	34.0	26.0	34.0
3	30.23291583146819	31.0	30.0	34.0	26.0	34.0
4	33.938018093045805	35.0	35.0	37.0	30.0	37.0
5	33.504894696830476	35.0	35.0	37.0	28.0	37.0
6	33.458319745313595	37.0	35.0	37.0	28.0	37.0
7	33.33489591134273	37.0	35.0	37.0	27.0	37.0
8	33.28723568867271	37.0	35.0	37.0	27.0	37.0
9	34.720140614316584	39.0	35.0	39.0	27.0	39.0
10	34.59560353646139	39.0	35.0	39.0	27.0	39.0
11	34.60451302838808	39.0	35.0	39.0	27.0	39.0
12	34.48006695043055	38.0	35.0	39.0	27.0	39.0
13	34.467387513326884	38.0	35.0	39.0	27.0	39.0
14	35.440642395542206	39.0	36.0	41.0	27.0	41.0
15	35.43288155602035	39.0	36.0	41.0	27.0	41.0
16	35.42004171831885	39.0	36.0	41.0	27.0	41.0
17	35.400446288992306	39.0	36.0	41.0	27.0	41.0
18	35.357658986912654	39.0	36.0	41.0	26.0	41.0
19	35.40392871698288	39.0	36.0	41.0	26.0	41.0
20	35.339641361259325	39.0	35.0	41.0	26.0	41.0
21	35.26755811157862	39.0	35.0	41.0	25.0	41.0
22	35.1904482683286	39.0	35.0	41.0	25.0	41.0
23	35.058865248980325	39.0	35.0	41.0	25.0	41.0
24	34.97982457910777	39.0	35.0	41.0	25.0	41.0
25	34.84246324330009	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76692.0
3	7.0
4	1199.0
5	10564.0
6	12122.0
7	9446.0
8	7124.0
9	6283.0
10	5700.0
11	5335.0
12	4944.0
13	4755.0
14	4625.0
15	4509.0
16	4659.0
17	4939.0
18	5461.0
19	6461.0
20	8170.0
21	10491.0
22	13326.0
23	16376.0
24	19339.0
25	22869.0
26	25916.0
27	29299.0
28	34433.0
29	41702.0
30	53287.0
31	68100.0
32	87857.0
33	114295.0
34	151855.0
35	203062.0
36	278610.0
37	421090.0
38	1049277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43880947232984	18.931474123355795	9.560127176767908	31.06958922754646
2	15.237029947464379	20.86333762838687	35.003269976867614	28.89636244728114
3	18.039309313415366	23.590511808875945	27.911553690588107	30.45862518712058
4	13.510449554753173	16.36473395722683	32.988156037615	37.136660450405
5	15.016275784460214	37.24034835529008	32.20050660970112	15.54286925054858
6	34.44824284453661	34.5540503598447	16.22247340293683	14.775233392681859
7	30.13275224644165	31.735474214564256	19.261274168036703	18.87049937095739
8	27.747651213946682	34.437424486864785	18.550275282116367	19.264649017072166
9	27.232815305027675	15.485151389611815	18.758183820379084	38.523849484981426
10	17.10542622373834	28.419601625112335	30.722611283865653	23.752360867283674
11	35.90139826140658	22.43181408544667	21.54523696293954	20.12155069020721
12	23.921757056389506	24.726345152465605	28.371547402465424	22.980350388679465
13	29.108564229329836	20.969672891289175	24.842082824765495	25.079680054615494
14	23.170171329492632	20.56779399505345	25.424036862819843	30.83799781263408
15	25.014927995002047	27.732637661613698	22.521333043391415	24.731101299992847
16	24.945150279028923	26.773108410526614	23.976374606951644	24.30536670349282
17	23.677572320568014	26.938858323283156	25.497439594470855	23.886129761677978
18	23.67654406585861	26.433459903703593	26.216851411248417	23.673144619189383
19	24.777161634832616	25.914547347398393	26.097336421130162	23.21095459663883
20	25.185453767747795	25.389125380556443	25.80712666518894	23.618294186506827
21	25.401625910307207	25.07397906063237	25.723252443894197	23.801142585166225
22	25.333260626859637	24.850671407695295	25.419481534385145	24.396586431059923
23	24.93208407693833	24.869621257188182	25.6694569558936	24.528837709979886
24	24.637599559198684	25.229182690332824	25.7022792204389	24.43093853002959
25	24.476927567710973	25.13056638411717	25.843649481618204	24.548856566553653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	809.0
1	809.0
2	895.0
3	981.0
4	981.0
5	981.0
6	1393.5
7	1806.0
8	1806.0
9	1806.0
10	2409.5
11	3013.0
12	3013.0
13	3013.0
14	4318.0
15	5623.0
16	5623.0
17	5623.0
18	11024.5
19	16426.0
20	16426.0
21	16426.0
22	26951.0
23	37476.0
24	37476.0
25	37476.0
26	57210.0
27	76944.0
28	76944.0
29	76944.0
30	100670.5
31	124397.0
32	124397.0
33	124397.0
34	152978.5
35	181560.0
36	181560.0
37	181560.0
38	213799.5
39	246039.0
40	246039.0
41	246039.0
42	278007.5
43	309976.0
44	309976.0
45	309976.0
46	339899.5
47	369823.0
48	369823.0
49	369823.0
50	384943.0
51	400063.0
52	400063.0
53	400063.0
54	380147.5
55	360232.0
56	360232.0
57	360232.0
58	321208.0
59	282184.0
60	282184.0
61	282184.0
62	239976.5
63	197769.0
64	197769.0
65	197769.0
66	156371.0
67	114973.0
68	114973.0
69	114973.0
70	85728.5
71	56484.0
72	56484.0
73	56484.0
74	39787.0
75	23090.0
76	23090.0
77	23090.0
78	16291.5
79	9493.0
80	9493.0
81	9493.0
82	6567.5
83	3642.0
84	3642.0
85	3642.0
86	2333.5
87	1025.0
88	1025.0
89	1025.0
90	666.5
91	308.0
92	308.0
93	308.0
94	172.0
95	36.0
96	36.0
97	36.0
98	21.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10300338611681484
2	0.0
3	0.04011785371961196
4	0.052227567728532785
5	0.06685128669252197
6	0.006479759250387457
7	0.0016287919427203444
8	0.005913222922484729
9	3.186766844452848E-4
10	1.7704260246960267E-4
11	0.006090265524954332
12	3.540852049392054E-5
13	0.0025494134755622783
14	0.05686608391323637
15	0.022130325308700335
16	0.0035054435288981328
17	6.019448483966491E-4
18	0.006727618893844902
19	0.005629954758533365
20	8.852130123480134E-4
21	0.014411267841025657
22	0.0013455237787689802
23	0.0035054435288981328
24	0.008179368234095643
25	0.16755311897723196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2824179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.61460633759327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.39247088728031	18.718328447304298
2	14.83596767456466	9.67738490686371
3	8.025977012964116	7.852922422571922
4	4.904599339226784	6.398463067700065
5	3.3644162755857048	5.4864556192009735
6	2.3652123223763857	4.628428127947833
7	1.722581048309712	3.932691194265399
8	1.2790847523522166	3.337347653630842
9	0.9849747286396598	2.891210672435424
>10	4.849555322701875	26.798253412693935
>50	0.19569526861813263	4.360596150195737
>100	0.07607643367578709	4.266405042737145
>500	0.001967767957503626	0.45127814369771085
>1k	0.0013118453049940134	0.9746852029868216
>5k	1.0932044208283444E-4	0.2255499357681762
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6326	0.22399430064454132	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4218	0.1493531394433568	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4163	0.1474056708161912	No Hit
GGTATCAACGCAGAGTACATGGGGG	3465	0.12269052351143465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	3.540852049392054E-5	0.0	0.0	0.0
7	0.0	3.540852049392054E-5	0.0	0.0	0.0
8	0.0	3.540852049392054E-5	0.0	0.0	0.0
9	0.0	3.540852049392054E-5	0.0	0.0	0.0
10	0.0	3.540852049392054E-5	0.0	0.0	0.0
11	0.0	3.540852049392054E-5	0.0	0.0	0.0
12	0.0	3.540852049392054E-5	0.0	0.0	3.540852049392054E-5
13	0.0	3.540852049392054E-5	0.0	3.540852049392054E-5	3.540852049392054E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	45	6.7383406E-4	14.785403	2
GGTATCA	1990	0.0	13.915164	1
TATACCG	75	2.0776564E-4	11.398405	5
GTATCAA	4360	0.0	10.890868	1
CGAATAC	70	0.0014856482	10.862744	3
TGCACCG	200	2.5465852E-11	9.973605	5
TAATACC	125	2.5554527E-6	9.884923	4
GTCTTAG	310	0.0	9.822832	1
GCACCGT	185	6.5847416E-10	9.749347	6
GGTCGTG	90	0.0011167985	9.497493	8
TCGCACG	90	0.0011209671	9.493625	18
TAGACAG	400	0.0	9.261205	5
TAAGGTA	155	4.2327338E-7	9.19813	4
CTTAGAC	300	0.0	9.188071	3
AACCGCT	135	6.917575E-6	9.1412	7
GTTCGCG	125	2.7773844E-5	9.11388	16
CTCTTAG	410	0.0	9.05168	1
CGAGCGC	105	4.4926628E-4	9.047957	10
TTGCGCG	105	4.5245944E-4	9.041548	18
AGACCGT	210	6.7848305E-10	9.040747	6
>>END_MODULE