FastQCFastQC Report
Mon 15 Aug 2016
SRR2921153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460842
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39150.1590918880610783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.013.2459391
CTAGATC1353.7471182E-1011.9583564
TCGTCAA700.001497246210.8525219
TATACCG909.558543E-510.5512765
TAAGGTA1303.8642065E-710.226834
TAACGAG750.002657433310.1292255
ATCGGAC1054.122428E-59.9489533
CTGTACG1054.1242696E-59.9485484
TTTATAC2251.8189894E-129.7077663
CCAATCG1002.761343E-49.49827215
CGCAATG800.00452988139.49672818
TTAGCGC1002.7669853E-49.4963424
CGGACCG800.004532169.4961495
CGATTCG900.00111834619.4959559
GGGACGC2201.6370905E-119.4957636
TATGCAC3150.09.3459863
AGGGACG2550.09.309955
GTATCAA35000.09.2853211
CTAGACA2151.07320375E-109.27549654
ACGCTCT2151.07320375E-109.275129
Produced by FastQC (version 0.11.5)